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Product Info Summary
| SKU: | PA1354 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Interleukin-10 IL10 Antibody Picoband®
SKU/Catalog Number
PA1354
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interleukin-10 IL10 Antibody catalog # PA1354. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interleukin-10 IL10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1354)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human IL10.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1354 is reactive to IL10 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
20517 MW
Background of IL-10
Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse Il 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1354 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Anti-IL-10 antibody, PA1354, Western blotting
Lane 1: Recombinant Human IL-10 Protein 10ng
Lane 2: Recombinant Human IL-10 Protein 5ng
Lane 3: Recombinant Human IL-10 Protein 2.5ng
Protein Target Info & Infographic
Gene/Protein Information For IL10 (Source: Uniprot.org, NCBI)
Gene Name
IL10
Full Name
Interleukin-10
Weight
20517 MW
Superfamily
IL-10 family
Alternative Names
Interleukin-10;IL-10;Cytokine synthesis inhibitory factor;CSIF;IL10; IL10 CSIF, GVHDS, IL-10A, TGIF, IL10 interleukin 10 interleukin-10|T-cell growth inhibitory factor|cytokine synthesis inhibitory factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL10, check out the IL10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Interleukin-10 IL10 Antibody Picoband® (PA1354)
Hello CJ!
PA1354 has been cited in 17 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Women with Recurrent Miscarriage Have Decreased Expression of 25-Hydroxyvitamin D3-1?-Hydroxylase by the Fetal-Maternal Interface
Protective Effect of the Total Saponins from Rosa laevigata Michx Fruit against Carbon Tetrachloride-Induced Liver Fibrosis in Rats
Pentoxifylline inhibits pulmonary inflammation induced by infrarenal aorticcross-clamping dependent of adenosine receptor A2A
Re-polarizing Myeloid-derived Suppressor Cells (MDSCs) with Cationic Polymers for Cancer Immunotherapy
Exogenous interleukin-10 alleviates allergic inflammation but inhibits local interleukin-10 expression in a mouse allergic rhinitis model
Wang Zh, Zeng Xy, Han Sp, Fan Gx, Wang Jy. Neurochem Res. 2012 Aug;37(8):1811-9. Doi: 10.1007/S11064-012-0795-0. Epub 2012 May 15. Interleukin-10 Of Red Nucleus Plays Anti-Allodynia Effect In Neuropathic Pain Rats With Spared Nerve Injury.
Shao Q, Li Y, Wang Q, Zhao J. Neurochem Res. 2015 Apr;40(4):733-9. Doi: 10.1007/S11064-015-1521-5. Epub 2015 Jan 24. Il-10 And Il-1?? Mediate Neuropathic-Pain Like Behavior In The Ventrolateral Orbital Cortex.
Wang R, Lu M, Zhang J, Chen S, Luo X, Qin Y, Chen H. J Exp Clin Cancer Res. 2011 May 20;30:62. Doi: 10.1186/1756-9966-30-62. Increased Il-10 Mrna Expression In Tumor-Associated Macrophage Correlated With Late Stage Of Lung Cancer.
Zhong B, Shen H, Sun X, Wang H, Zhang Y, Sun Z. Cancer Biother Radiopharm. 2012 May;27(4):252-8. Doi: 10.1089/Cbr.2011.1105. Additive Effects Of Ulinastatin And Docetaxel On Growth Of Breast Cancer Xenograft In Nude Mice And Expression Of Pge2, Il...
Zhan X, Jia L, Niu Y, Qi H, Chen X, Zhang Q, Zhang J, Wang Y, Dong L, Wang C. Biomaterials. 2014 Dec;35(38):10046-57. Doi: 10.1016/J.Biomaterials.2014.09.007. Epub 2014 Sep 22. Targeted Depletion Of Tumour-Associated Macrophages By An Alendronate-...
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Interleukin-10 IL10 Antibody Picoband®
Question
Would PA1354 anti-IL10 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
It shows on the product datasheet, PA1354 anti-IL10 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-04
Question
We want to test anti-IL10 antibody PA1354 on human esophagus for research purposes, then I may be interested in using anti-IL10 antibody PA1354 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
The products we sell, including anti-IL10 antibody PA1354, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-06
Question
I see that the anti-IL10 antibody PA1354 works with WB, what is the protocol used to produce the result images on the product page?
O. Edwards
Verified customer
Asked: 2019-09-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-27
Question
We were well pleased with the WB result of your anti-IL10 antibody. However we have observed positive staining in esophagus secreted. using this antibody. Is that expected? Could you tell me where is IL10 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
From literature, esophagus does express IL10. Generally IL10 expresses in secreted. Regarding which tissues have IL10 expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 1847510
Boster Scientific Support
Answered: 2019-06-25
Question
I was wanting to use your anti-IL10 antibody for WB for human esophagus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human esophagus identification?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
It shows on the product datasheet, PA1354 anti-IL10 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human esophagus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-24
Question
Is there a BSA free version of anti-IL10 antibody PA1354 available?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL10 antibody PA1354 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-29
Question
I have attached the WB image, lot number and protocol we used for esophagus using anti-IL10 antibody PA1354. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-16
Question
We are currently using anti-IL10 antibody PA1354 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
The anti-IL10 antibody (PA1354) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-14
Question
Is this PA1354 anti-IL10 antibody reactive to the isotypes of IL10?
Verified Customer
Verified customer
Asked: 2018-12-27
Answer
The immunogen of PA1354 anti-IL10 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human IL10(46-60aa DAFSRVKTFFQMKDQ). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-27
Question
Is a blocking peptide available for product anti-IL10 antibody (PA1354)?
M. Kulkarni
Verified customer
Asked: 2018-08-31
Answer
We do provide the blocking peptide for product anti-IL10 antibody (PA1354). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-08-31
Question
Does anti-IL10 antibody PA1354 work for WB with esophagus?
A. Carter
Verified customer
Asked: 2018-06-19
Answer
According to the expression profile of esophagus, IL10 is highly expressed in esophagus. So, it is likely that anti-IL10 antibody PA1354 will work for WB with esophagus.
Boster Scientific Support
Answered: 2018-06-19
Question
My question regarding product PA1354, anti-IL10 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Thomas
Verified customer
Asked: 2016-06-16
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1354 anti-IL10 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-06-16
Question
Will anti-IL10 antibody PA1354 work on zebrafish WB with t-cell?
L. Edwards
Verified customer
Asked: 2013-10-29
Answer
Our lab technicians have not tested anti-IL10 antibody PA1354 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-IL10 antibody PA1354 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-10-29
Question
We have seen staining in human esophagus. Do you have any suggestions? Is anti-IL10 antibody supposed to stain esophagus positively?
E. Johnson
Verified customer
Asked: 2013-09-19
Answer
Based on literature esophagus does express IL10. Based on Uniprot.org, IL10 is expressed in esophagus, t-cell, among other tissues. Regarding which tissues have IL10 expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 1847510
Boster Scientific Support
Answered: 2013-09-19
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagus using anti-IL10 antibody PA1354. Let me know if you need anything else.
E. Parker
Verified customer
Asked: 2013-08-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-08-06
