Click image to see more details
Product Info Summary
| SKU: | M00570 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD2 Monoclonal Antibody
SKU/Catalog Number
M00570
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD2 Monoclonal Antibody catalog # M00570. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00570)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFBD-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00570 is reactive to CD2 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
26 kDa
Calculated molecular weight
84142 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00570 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:30
Positive Control
WB: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of CD2 expression in Jurkat cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CD2 (Source: Uniprot.org, NCBI)
Gene Name
CD2
Full Name
T-cell surface antigen CD2
Weight
84142 MW
Alternative Names
Disintegrin and metalloproteinase domain-containing protein 10;ADAM 10;3.4.24.81;CDw156;Kuzbanian protein homolog;Mammalian disintegrin-metalloprotease;CD156c;ADAM10;KUZ, MADM; CD2 LFA-2, SRBC, T11 CD2 molecule T-cell surface CD2|CD2 (p50), sheep red blood cell receptor|LFA-3 receptor|T-cell surface T11/Leu-5|erythrocyte receptor|lymphocyte-function -2|rosette receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD2, check out the CD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD2 Monoclonal Antibody (M00570)
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No publications found for M00570
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CD2 Monoclonal Antibody
Question
Is there a BSA free version of anti-CD2 Monoclonal antibody M00570 available?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD2 Monoclonal antibody M00570 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-24
Question
Our team were satisfied with the WB result of your anti-CD2 Monoclonal antibody. However we have observed positive staining in leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is CD2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
According to literature, leukocyte does express CD2. Generally CD2 expresses in cell membrane. Regarding which tissues have CD2 expression, here are a few articles citing expression in various tissues:
Pancreas, and Spleen, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-17
Question
Here is the WB image, lot number and protocol we used for leukocyte using anti-CD2 Monoclonal antibody M00570. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-13
Question
Is a blocking peptide available for product anti-CD2 Monoclonal antibody (M00570)?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
We do provide the blocking peptide for product anti-CD2 Monoclonal antibody (M00570). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-16
Question
Our lab used your anti-CD2 Monoclonal antibody for WB on leukocyte a few years ago. I am using human, and We are going to use the antibody for IF next. My lab would like examining leukocyte as well as pancreas spleen in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
I looked at the website and datasheets of our anti-CD2 Monoclonal antibody and it appears that M00570 has been validated on human in both WB and IF. Thus M00570 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-04
Question
We are currently using anti-CD2 Monoclonal antibody M00570 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
The anti-CD2 Monoclonal antibody (M00570) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-28
Question
I have a question about product M00570, anti-CD2 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00570 anti-CD2 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-30
Question
I would like using your anti-CD2 Monoclonal antibody for apoptotic process studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-04-08
Answer
We appreciate your inquiry. This M00570 anti-CD2 Monoclonal antibody is validated on jurkat cell lysate. It is guaranteed to work for IP, IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-04-08
Question
I was wanting to use your anti-CD2 Monoclonal antibody for IP for human leukocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukocyte identification?
Verified Customer
Verified customer
Asked: 2018-08-03
Answer
It shows on the product datasheet, M00570 anti-CD2 Monoclonal antibody has been validated for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-03
Question
Is this M00570 anti-CD2 Monoclonal antibody reactive to the isotypes of CD2?
Verified Customer
Verified customer
Asked: 2018-03-01
Answer
The immunogen of M00570 anti-CD2 Monoclonal antibody is A synthesized peptide derived from human CD2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-03-01
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-CD2 Monoclonal antibody M00570. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-02-13
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-13
Question
Would M00570 anti-CD2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-11-28
Answer
As indicated on the product datasheet, M00570 anti-CD2 Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-28
Question
I am looking for to test anti-CD2 Monoclonal antibody M00570 on human leukocyte for research purposes, then I may be interested in using anti-CD2 Monoclonal antibody M00570 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Lewis
Verified customer
Asked: 2017-06-19
Answer
The products we sell, including anti-CD2 Monoclonal antibody M00570, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-19
Question
We have observed staining in human pancreas spleen. Are there any suggestions? Is anti-CD2 Monoclonal antibody supposed to stain pancreas spleen positively?
A. Wu
Verified customer
Asked: 2017-01-25
Answer
According to literature pancreas spleen does express CD2. According to Uniprot.org, CD2 is expressed in leukocyte, pancreas spleen, among other tissues. Regarding which tissues have CD2 expression, here are a few articles citing expression in various tissues:
Pancreas, and Spleen, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-01-25
Question
I see that the anti-CD2 Monoclonal antibody M00570 works with IP, what is the protocol used to produce the result images on the product page?
J. Williams
Verified customer
Asked: 2015-06-29
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-06-29
Question
Will anti-CD2 Monoclonal antibody M00570 work for IP with leukocyte?
D. Lewis
Verified customer
Asked: 2013-08-05
Answer
According to the expression profile of leukocyte, CD2 is highly expressed in leukocyte. So, it is likely that anti-CD2 Monoclonal antibody M00570 will work for IP with leukocyte.
Boster Scientific Support
Answered: 2013-08-05
