Click image to see more details
Product Info Summary
| SKU: | A00021 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IL10 Antibody Picoband®
SKU/Catalog Number
A00021
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL10 Antibody Picoband® catalog # A00021. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00021)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse IL10 recombinant protein (Position: S19-S178).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00021 is reactive to Il10 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
19 kDa
Calculated molecular weight
24993 MW
Background of IL-10
CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein receptor that down regulates the immune system. It is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00021 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot,0.1-0.5μg/ml
ELISA (Cap),1-5μg/ml
Positive Control
WB: mouse brain tissue, mouse spleen tissue, mouse heart tissue, mouse liver tissue, mouse kidney tissue, mouse testis tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of IL10 using anti-IL10 antibody (A00021).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse brain tissue lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: mouse liver tissue lysates,
Lane 5: mouse kidney tissue lysates,
Lane 6: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (Catalog # A00021) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL10 at approximately 19KD. The expected band size for IL10 is at 21KD.
Click image to see more details
Figure 2. Sandwich ELISA - Recombinant mouse IL10 protein standard curve.
Use in combination with reagents from Mouse IL10 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0417).
Protein Target Info & Infographic
Gene/Protein Information For Il10 (Source: Uniprot.org, NCBI)
Gene Name
Il10
Full Name
Interleukin-10
Weight
24993 MW
Superfamily
IL-10 family
Alternative Names
Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; Il10; Il-10 IL10 CSIF, GVHDS, IL-10A, TGIF, IL10 interleukin 10 interleukin-10|T-cell growth inhibitory factor|cytokine synthesis inhibitory factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Il10, check out the Il10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Il10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL10 Antibody Picoband® (A00021)
Hello CJ!
A00021 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Hydrogen sulfide protects blood%u2013brain barrier integrity following cerebral ischemia
Women with Recurrent Miscarriage Have Decreased Expression of 25-Hydroxyvitamin D3-1?-Hydroxylase by the Fetal-Maternal Interface
Protective Effect of the Total Saponins from Rosa laevigata Michx Fruit against Carbon Tetrachloride-Induced Liver Fibrosis in Rats
Impact of bone marrow-derived mesenchymal stem cells on remodeling the lung injury induced by lipopolysaccharides in mice
Pentoxifylline inhibits pulmonary inflammation induced by infrarenal aorticcross-clamping dependent of adenosine receptor A2A
Re-polarizing Myeloid-derived Suppressor Cells (MDSCs) with Cationic Polymers for Cancer Immunotherapy
Exogenous interleukin-10 alleviates allergic inflammation but inhibits local interleukin-10 expression in a mouse allergic rhinitis model
Wang R, Lu M, Zhang J, Chen S, Luo X, Qin Y, Chen H. J Exp Clin Cancer Res. 2011 May 20;30:62. Doi: 10.1186/1756-9966-30-62. Increased Il-10 Mrna Expression In Tumor-Associated Macrophage Correlated With Late Stage Of Lung Cancer.
Wang T, Zhou Yt, Chen Xn, Zhu Ax. Braz J Med Biol Res. 2014 Sep;47(9):738-45. Epub 2014 Jul 25. Putative Role Of Ischemic Postconditioning In A Rat Model Of Limb Ischemia And Reperfusion: Involvement Of Hypoxia-Inducible Factor-1?? Expression.
Gong T, Wang Cf, Yuan Jr, Li Y, Gu Jf, Zhao Bj, Zhang L, Jia Xb, Feng L, Liu Sl. Evid Based Complement Alternat Med. 2015;2015:630760. Doi: 10.1155/2015/630760. Epub 2015 May 3. Inhibition Of Tumor Growth And Immunomodulatory Effects Of Flavonoids...
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-IL10 Antibody Picoband®
Question
Our lab want to know about using your anti-IL10 antibody for negative regulation of mhc class ii biosynthetic process studies. Has this antibody been tested with western blotting on brain tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
We appreciate your inquiry. This A00021 anti-IL10 antibody is validated on mouse heart tissue, brain tissue, spleen tissue, liver tissue, kidney tissue, testis tissue. It is guaranteed to work for ELISA, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-14
Question
Is a blocking peptide available for product anti-IL10 antibody (A00021)?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
We do provide the blocking peptide for product anti-IL10 antibody (A00021). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-07
Question
Will A00021 anti-IL10 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
You can see on the product datasheet, A00021 anti-IL10 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-17
Question
Does anti-IL10 antibody A00021 work for WB with esophagus?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
According to the expression profile of esophagus, IL10 is highly expressed in esophagus. So, it is likely that anti-IL10 antibody A00021 will work for WB with esophagus.
Boster Scientific Support
Answered: 2020-01-09
Question
I was wanting to use your anti-IL10 antibody for WB for rat esophagus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat esophagus identification?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
As indicated on the product datasheet, A00021 anti-IL10 antibody has been validated for ELISA, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat esophagus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-08
Question
Here is the WB image, lot number and protocol we used for esophagus using anti-IL10 antibody A00021. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-12
Question
Can you help my question with product A00021, anti-IL10 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00021 anti-IL10 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-06
Question
Do you have a BSA free version of anti-IL10 antibody A00021 available?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL10 antibody A00021 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-27
Question
We have observed staining in rat t-cell. Do you have any suggestions? Is anti-IL10 antibody supposed to stain t-cell positively?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
Based on literature t-cell does express IL10. Based on Uniprot.org, IL10 is expressed in esophagus, t-cell, among other tissues. Regarding which tissues have IL10 expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 1847510
Boster Scientific Support
Answered: 2019-10-31
Question
We want to test anti-IL10 antibody A00021 on rat esophagus for research purposes, then I may be interested in using anti-IL10 antibody A00021 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
The products we sell, including anti-IL10 antibody A00021, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-24
Question
I see that the anti-IL10 antibody A00021 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-11
Question
My team were content with the WB result of your anti-IL10 antibody. However we have been able to see positive staining in esophagus secreted. using this antibody. Is that expected? Could you tell me where is IL10 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
Based on literature, esophagus does express IL10. Generally IL10 expresses in secreted. Regarding which tissues have IL10 expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 1847510
Boster Scientific Support
Answered: 2019-09-18
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagus using anti-IL10 antibody A00021. Let me know if you need anything else.
R. Kulkarni
Verified customer
Asked: 2019-07-29
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-29
Question
We are currently using anti-IL10 antibody A00021 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-02
Answer
The anti-IL10 antibody (A00021) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-02
Question
Our lab used your anti-IL10 antibody for WB on t-cell in a previous project. I am using mouse, and We are going to use the antibody for ELISA next. I would like examining t-cell as well as esophagus in our next experiment. Do you have any suggestion on which antibody would work the best for ELISA?
P. Jones
Verified customer
Asked: 2018-03-08
Answer
I have checked the website and datasheets of our anti-IL10 antibody and I see that A00021 has been validated on mouse in both WB and ELISA. Thus A00021 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-03-08
Question
Is this A00021 anti-IL10 antibody reactive to the isotypes of IL10?
M. Jones
Verified customer
Asked: 2013-07-09
Answer
The immunogen of A00021 anti-IL10 antibody is E. coli-derived mouse IL10 recombinant protein (Position: S19-S178). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-07-09
