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Product Info Summary
| SKU: | M00118 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IKK beta IKBKB Rabbit Monoclonal Antibody
SKU/Catalog Number
M00118
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IKK beta IKBKB Rabbit Monoclonal Antibody catalog # M00118. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IKK beta IKBKB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00118)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOOA-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IKK beta
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00118 is reactive to IKBKB in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
87 kDa
Calculated molecular weight
86564 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00118 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human Hela whole cell, human Raji whole cell, human A549 whole cell, rat heart tissue, mouse heart tissue
IHC: human kidney tissue
ICC/IF: HeLa cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IKBKB using anti-IKBKB antibody (M00118).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKBKB antigen affinity purified monoclonal antibody (Catalog # M00118) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKBKB at approximately 87 kDa. The expected band size for IKBKB is at 87 kDa.
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Immunohistochemical analysis of paraffin-embedded human kidney, using IKK beta Antibody.
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Immunofluorescent analysis of HeLa cells, using IKK beta Antibody.
Protein Target Info & Infographic
Gene/Protein Information For IKBKB (Source: Uniprot.org, NCBI)
Gene Name
IKBKB
Full Name
Inhibitor of nuclear factor kappa-B kinase subunit beta
Weight
86564 MW
Superfamily
protein kinase superfamily
Alternative Names
Inhibitor of nuclear factor kappa-B kinase subunit beta;I-kappa-B-kinase beta;IKK-B;IKK-beta;IkBKB;2.7.11.10;I-kappa-B kinase 2;IKK2;Nuclear factor NF-kappa-B inhibitor kinase beta;NFKBIKB;IKBKB;IKKB; IKBKB IKK-beta, IKK2, IKKB, IMD15, IMD15A, IMD15B, NFKBIKB inhibitor of nuclear factor kappa B kinase subunit beta inhibitor of nuclear factor kappa-B kinase subunit beta|I-kappa-B kinase 2|I-kappa-B-kinase beta|inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta|nuclear factor NF-kappa-B inhibitor kinase beta|serine/threonine protein kinase IKBKB
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IKBKB, check out the IKBKB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IKBKB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IKK beta IKBKB Rabbit Monoclonal Antibody (M00118)
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