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Product Info Summary
| SKU: | M01918-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IKK alpha CHUK Rabbit Monoclonal Antibody
SKU/Catalog Number
M01918-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IKK alpha CHUK Rabbit Monoclonal Antibody catalog # M01918-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IKK alpha CHUK Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01918-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GOD-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IKK alpha
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01918-1 is reactive to CHUK in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
85 kDa
Calculated molecular weight
84640 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01918-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:20000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: human Hela whole cell, human 293T whole cell, human A375 whole cell, human Daudi whole cell, rat liver tissue, rat lung tissue, mouse liver tissue, mouse lung tissue
IHC: mouse kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IKK alpha using anti-IKK alpha antibody (M01918-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human A375 whole cell lysates,
Lane 4: human Daudi whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKK alpha antigen affinity purified monoclonal antibody (Catalog # M01918-1) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKK alpha at approximately 85 kDa. The expected band size for IKK alpha is at 85 kDa.
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Immunohistochemical analysis of paraffin-embedded mouse kidney, using IKK alpha Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CHUK (Source: Uniprot.org, NCBI)
Gene Name
CHUK
Full Name
Inhibitor of nuclear factor kappa-B kinase subunit alpha
Weight
84640 MW
Superfamily
protein kinase superfamily
Alternative Names
Inhibitor of nuclear factor kappa-B kinase subunit alpha;I-kappa-B kinase alpha;IKK-A;IKK-alpha;IkBKA;IkappaB kinase;2.7.11.10;Conserved helix-loop-helix ubiquitous kinase;I-kappa-B kinase 1;IKK1;Nuclear factor NF-kappa-B inhibitor kinase alpha;NFKBIKA;Transcription factor 16;TCF-16;CHUK;IKKA, TCF16; CHUK IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16 component of inhibitor of nuclear factor kappa B kinase complex inhibitor of nuclear factor kappa-B kinase subunit alpha|I-kappa-B kinase 1|I-kappa-B kinase-alpha|IKK-a kinase|IkB kinase alpha subunit|Nuclear factor NFkappaB inhibitor kinase alpha|TCF-16|conserved helix-loop-helix ubiquitous kinase|transcription factor 16
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHUK, check out the CHUK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHUK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IKK alpha CHUK Rabbit Monoclonal Antibody (M01918-1)
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