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Product Info Summary
| SKU: | EKC1293 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
IKK-alpha/beta Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC1293
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The IKK-alpha/beta Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor IKK-alpha/beta protein expression profile in cells. The kit can be used for measuring the relative amounts of IKK-alpha/beta in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on IKK-alpha/beta.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
IKK-alpha/beta Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1293)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1293 is reactive to CHUK in Human, Mouse, Rat samples
Validated Sample Types
Cell lines
Application Guarantee
EKC1293 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-IKK-alpha/beta Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from Jurkat cells
Protein Target Info & Infographic
Gene/Protein Information For CHUK (Source: Uniprot.Org, NCBI)
Gene Name
CHUK
Full Name
Inhibitor of nuclear factor kappa-B kinase subunit alpha
Weight
84640 MW
Superfamily
protein kinase superfamily
Alternative Names
CHUK;Conserved helix-loop-helix ubiquitous kinase;I kappa-B kinase alpha;I-kappa-B kinase 1;IkappaB kinase;IkBKA;IKK-A;IKK-alpha;IKK1;IKKA;Inhibitor of nuclear factor kappa-B kinase alpha subunit;kinase IKK-alpha;NFKBIKA;Nuclear factor NFkappaB inhibitor kinase alpha;I-kappa-B kinase 2;I-kappa-B-kinase beta;IkBKB;IKK-B;IKK-beta;IKK2;IKKB;kinase IKK-beta;NFKBIKB;Nuclear factor NF-kappa-B inhibitor kinase beta CHUK IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16 component of inhibitor of nuclear factor kappa B kinase complex inhibitor of nuclear factor kappa-B kinase subunit alpha|I-kappa-B kinase 1|I-kappa-B kinase-alpha|IKK-a kinase|IkB kinase alpha subunit|Nuclear factor NFkappaB inhibitor kinase alpha|TCF-16|conserved helix-loop-helix ubiquitous kinase|transcription factor 16
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CHUK, check out the CHUK Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CHUK: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For IKK-alpha/beta Colorimetric Cell-Based ELISA Kit (EKC1293)
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Customer Q&As
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1 Customer Q&As for IKK-alpha/beta Colorimetric Cell-Based ELISA Kit
Question
I used your product EKC1293 IKK-alpha/beta Colorimetric Cell-Based ELISA Kit antibody in WB of various human fibroblast cell lines. However, I only detected a single band, but it is at 130kDa, rather than 85kDa, is this the correct band?
Verified Customer
Verified customer
Asked: 2017-06-09
Answer
The EKC1293 IKK-alpha/beta Colorimetric Cell-Based ELISA Kit antibody target the containing leucine zipper associates with many proteins. It is possible that the band is dimerization. The leucine zipper is essential to allow homo- and hetero-dimerization. We recommend to add DTT to lysis buffer as we find this would help.
Boster Scientific Support
Answered: 2017-06-09
