IKK-alpha Colorimetric Cell-Based ELISA Kit

CHUK ELISA kit, cell-based

The IKK-alpha Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor IKK-alpha protein expression profile in cells. The kit can be used for measuring the relative amounts of IKK-alpha in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on IKK-alpha.

Product Info Summary

SKU: EKC1292
Size: 1 kit, containing one 96-well plate and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA
Sample Types: Cell lines

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Product Name

IKK-alpha Colorimetric Cell-Based ELISA Kit

See all CHUK products

SKU/Catalog Number

EKC1292

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The IKK-alpha Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor IKK-alpha protein expression profile in cells. The kit can be used for measuring the relative amounts of IKK-alpha in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on IKK-alpha.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

IKK-alpha Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1292)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1292 is reactive to CHUK in Human, Mouse, Rat samples

Validated Sample Types

Cell lines

Application Guarantee

EKC1292 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-IKK-alpha Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For CHUK (Source: Uniprot.Org, NCBI)

Gene Name

CHUK

Full Name

Inhibitor of nuclear factor kappa-B kinase subunit alpha

Weight

84640 MW

Superfamily

protein kinase superfamily

Alternative Names

CHUK;Conserved helix-loop-helix ubiquitous kinase;I kappa-B kinase alpha;I-kappa-B kinase 1;IkappaB kinase;IkBKA;IKK-A;IKK-alpha;IKK1;IKKA;Inhibitor of nuclear factor kappa-B kinase alpha subunit;kinase IKK-alpha;NFKBIKA;Nuclear factor NFkappaB inhibitor kinase alpha CHUK IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16 component of inhibitor of nuclear factor kappa B kinase complex inhibitor of nuclear factor kappa-B kinase subunit alpha|I-kappa-B kinase 1|I-kappa-B kinase-alpha|IKK-a kinase|IkB kinase alpha subunit|Nuclear factor NFkappaB inhibitor kinase alpha|TCF-16|conserved helix-loop-helix ubiquitous kinase|transcription factor 16

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CHUK, check out the CHUK Infographic

CHUK infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CHUK: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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3 Customer Q&As for IKK-alpha Colorimetric Cell-Based ELISA Kit

Question

Do the EKC1292 IKK-alpha Colorimetric Cell-Based ELISA Kit recognize an extracellular epitope or do you have to follow an intracellular protocol for flow?

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

For EKC1292 IKK-alpha Colorimetric Cell-Based ELISA Kit and most of our antibodies we are not able to provide specific epitope information but it seems most targets are intracellular. Therefore, not on the membrane and cells need fixation prior to staining intracellular target. Flow cytometry protocol is recommended.

Boster Scientific Support

Answered: 2019-12-13

Question

I have a question regarding your EKC1292 IKK-alpha Colorimetric Cell-Based ELISA Kit. I want to use antibody for flow cytometric analysis of phosphorylated IKK-alpha, do this EKC1292 IKK-alpha Colorimetric Cell-Based ELISA Kit detect phosphorylated IKK-alpha or only IKK-alpha? Since I want to study the TLR signaling pathway and I need to look at activated (phosphorylated) IIKK alpha enzyme. Can you guide me regarding this...so I can order an antibody accordingly?

Verified Customer

Verified customer

Asked: 2013-02-20

Answer

We can confirmed that EKC1292 IKK-alpha Colorimetric Cell-Based ELISA Kit has not been specifically raised against a phosphorylated residue of the protein, it is therefore likely to detect both phosphorylated and non-phosphorylated forms of the protein. We recommend to review the datasheets for further details and to select the tested antibody in the species you will be using. In regards to flow cytometry, unfortunately EKC1292 IKK-alpha Colorimetric Cell-Based ELISA Kit has not been tested, so we cannot guarantee.

Boster Scientific Support

Answered: 2013-02-20

Question

Hello; What is the concentration of EKC1292 IKK-alpha Colorimetric Cell-Based ELISA Kit?

Verified Customer

Verified customer

Asked: 2013-01-10

Answer

The concentration for EKC1292 IKK-alpha Colorimetric Cell-Based ELISA Kit is 0.25mg/ml.

Boster Scientific Support

Answered: 2013-01-10

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