Anti-IKK alpha CHUK Antibody

Product Name

Anti-IKK alpha CHUK Antibody

View all CHUK Antibodies

SKU/Catalog Number

A01918

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-IKK alpha CHUK Antibody (Catalog # A01918). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.

Storage & Handling

IKK alpha antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-IKK alpha CHUK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01918)

Host

Rabbit

Contents

IKK alpha Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

IKK alpha antibody was raised against a 19 amino acid peptide near the carboxy terminus of human IKK alpha. The immunogen is located within the last 50 amino acids of IKK alpha.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Antibody has no cross response to IKKb or IKKg.

Reactive Species

A01918 is reactive to CHUK in Human

Applications

A01918 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

84640 MW

Background of CHUK

Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The long-sought IκB kinase (IKK), which phosphorylates IκB, and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα and IKKβ). IKKα and IKKβ interact with each other and both are essential for the NF-κB activation. IKKα specifically phosphorylates IkB-alpha. IKKα is expressed in variety of human tissues.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IKK alpha can be used for detection of IKK alpha by Western blot at 1 μg/mL. An 85 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of IKK alpha in HeLa whole cell lysate with IKK alpha antibody at 1:1000 dilution.

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Immunocytochemistry of IKK alpha in Jurkat cells with IKK alpha antibody at 1μg/mL.

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Immunofluorescence of IKK alpha in Jurkat cells with IKK alpha antibody at 10 μg/mL.

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Immunofluorescence of IKKa in Jurkat cells with IKK alpha antibody at 20 μg/mL.

Green: IKK alpha Antibody (A01918)
Blue: DAPI staining

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Immunocytochemistry of IKKa in Jurkat cells with IKK alpha antibody at 2 μg/mL.

Gene/Protein Information For CHUK (Source: Uniprot.org, NCBI)

Alternative Names

CHUK; conserved helix-loop-helix ubiquitous kinaseIKBKA; EC 2.7.11; EC 2.7.11.10; I-kappa-B kinase 1; I-kappa-B kinase alpha; IkappaB kinase; IkBKA; IkBKalpha; IKK alpha; IKK1; IKK1I-kappa-B kinase-alpha; IKK-A; IKKAIkB kinase alpha subunit; IKK-alphainhibitor of nuclear factor kappa-B kinase subunit alpha; NFKBIKA; NFKBIKAIKK-a kinase; Nuclear factor NFkappaB inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase alpha; TCF16; TCF-16; Transcription factor 16 CHUK IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16 component of inhibitor of nuclear factor kappa B kinase complex inhibitor of nuclear factor kappa-B kinase subunit alpha|I-kappa-B kinase 1|I-kappa-B kinase-alpha|IKK-a kinase|IkB kinase alpha subunit|Nuclear factor NFkappaB inhibitor kinase alpha|TCF-16|conserved helix-loop-helix ubiquitous kinase|transcription factor 16

For more info on CHUK, check out the CHUK Infographic

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In this infographic, you will see the following information for CHUK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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