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Product Info Summary
| SKU: | M07073 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IKB epsilon NFKBIE Monoclonal Antibody
View all IkB-epsilon Antibodies
SKU/Catalog Number
M07073
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IKB epsilon NFKBIE Monoclonal Antibody catalog # M07073. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IKB epsilon NFKBIE Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07073)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADCF-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IKB epsilon Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M07073 is reactive to NFKBIE in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
116 kDa
Calculated molecular weight
31052 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07073 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of IKB epsilon expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For NFKBIE (Source: Uniprot.org, NCBI)
Gene Name
NFKBIE
Full Name
NF-kappa-B inhibitor epsilon
Weight
31052 MW
Superfamily
NF-kappa-B inhibitor family
Alternative Names
MAD2L1-binding protein;Caught by MAD2 protein;MAD2L1BP;CMT2, KIAA0110; NFKBIE IKBE NFKB inhibitor epsilon NF-kappa-B inhibitor epsilon|I-kappa-B-epsilon|NF-kappa-BIE|ikB-E|ikB-epsilon|ikappaBepsilon|nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NFKBIE, check out the NFKBIE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NFKBIE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IKB epsilon NFKBIE Monoclonal Antibody (M07073)
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No publications found for M07073
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Customer Q&As
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6 Customer Q&As for Anti-IKB epsilon NFKBIE Monoclonal Antibody
Question
Will anti-IKB epsilon Monoclonal antibody M07073 work on bovine IP with testis?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
Our lab technicians have not validated anti-IKB epsilon Monoclonal antibody M07073 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-IKB epsilon Monoclonal antibody M07073 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine testis in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-14
Question
Would anti-IKB epsilon Monoclonal antibody M07073 work for IP with b-cell?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
According to the expression profile of b-cell, NFKBIE is highly expressed in b-cell. So, it is likely that anti-IKB epsilon Monoclonal antibody M07073 will work for IP with b-cell.
Boster Scientific Support
Answered: 2019-10-28
Question
I am interested in to test anti-IKB epsilon Monoclonal antibody M07073 on human b-cell for research purposes, then I may be interested in using anti-IKB epsilon Monoclonal antibody M07073 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-03-12
Answer
The products we sell, including anti-IKB epsilon Monoclonal antibody M07073, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-12
Question
I have a question about product M07073, anti-IKB epsilon Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Bhatt
Verified customer
Asked: 2018-11-15
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M07073 anti-IKB epsilon Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-15
Question
Is this M07073 anti-IKB epsilon Monoclonal antibody reactive to the isotypes of NFKBIE?
H. Bhatt
Verified customer
Asked: 2017-05-02
Answer
The immunogen of M07073 anti-IKB epsilon Monoclonal antibody is A synthesized peptide derived from human IKB epsilon Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-05-02
Question
We are currently using anti-IKB epsilon Monoclonal antibody M07073 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
E. Wu
Verified customer
Asked: 2014-04-22
Answer
The anti-IKB epsilon Monoclonal antibody (M07073) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-04-22
