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Product Info Summary
| SKU: | A08339-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-IL-31 Antibody Picoband®
SKU/Catalog Number
A08339-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL-31 Antibody Picoband® catalog # A08339-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL-31 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08339-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human IL-31 recombinant protein (Position: S24-T164).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08339-1 is reactive to IL31 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
29 kDa
Calculated molecular weight
18.12kDa
Background of IL-31
Interleukin-31 (IL-31) is a protein that in humans is encoded by the IL31 gene that resides on chromosome 12. IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA and OSMR that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08339-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
WB: rat spleen tissue, rat thymus tissue
IHC: rat spleen tissue, human colon cancer tissue, mouse spleen tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of IL31 using anti-IL31 antibody (A08339-1).
IL31 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IL31 Antibody (A08339-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 2. IHC analysis of IL31 using anti-IL31 antibody (A08339-1).
IL31 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IL31 Antibody (A08339-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of IL31 using anti-IL31 antibody (A08339-1).
IL31 was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IL31 Antibody (A08339-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. Western blot analysis of IL31 using anti-IL31 antibody (A08339-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL31 antigen affinity purified polyclonal antibody (Catalog # A08339-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL31 at approximately 29KD. The expected band size for IL31 is at 18KD.
Protein Target Info & Infographic
Gene/Protein Information For IL31 (Source: Uniprot.org, NCBI)
Gene Name
IL31
Full Name
Interleukin-31
Weight
18.12kDa
Alternative Names
Interleukin-31; IL-31 Il31|1700013B14Rik|interleukin 31|interleukin-31|IL-31
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL31, check out the IL31 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL31: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL-31 Antibody Picoband® (A08339-1)
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Customer Q&As
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16 Customer Q&As for Anti-IL-31 Antibody Picoband®
Question
Will A08339-1 anti-IL-31 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
You can see on the product datasheet, A08339-1 anti-IL-31 antibody as been validated on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-15
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for peripheral blood using anti-IL-31 antibody A08339-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-02
Question
Is this A08339-1 anti-IL-31 antibody reactive to the isotypes of IL31?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
The immunogen of A08339-1 anti-IL-31 antibody is E.coli-derived human IL-31 recombinant protein (Position: S24-T164). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-05
Question
Do you have a BSA free version of anti-IL-31 antibody A08339-1 available?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-IL-31 antibody A08339-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-22
Question
My colleagues were happy with the WB result of your anti-IL-31 antibody. However we have been able to see positive staining in testis secreted. using this antibody. Is that expected? Could you tell me where is IL31 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
From literature, testis does express IL31. Generally IL31 expresses in secreted. Regarding which tissues have IL31 expression, here are a few articles citing expression in various tissues:
Peripheral blood, Pubmed ID: 15184896
Boster Scientific Support
Answered: 2019-11-15
Question
Does anti-IL-31 antibody A08339-1 work for IHC-P with peripheral blood?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
According to the expression profile of peripheral blood, IL31 is highly expressed in peripheral blood. So, it is likely that anti-IL-31 antibody A08339-1 will work for IHC-P with peripheral blood.
Boster Scientific Support
Answered: 2019-10-04
Question
I am interested in to test anti-IL-31 antibody A08339-1 on mouse peripheral blood for research purposes, then I may be interested in using anti-IL-31 antibody A08339-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
The products we sell, including anti-IL-31 antibody A08339-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-23
Question
See below the WB image, lot number and protocol we used for peripheral blood using anti-IL-31 antibody A08339-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-19
Question
I was wanting to use your anti-IL-31 antibody for IHC-P for mouse peripheral blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse peripheral blood identification?
Verified Customer
Verified customer
Asked: 2019-03-04
Answer
It shows on the product datasheet, A08339-1 anti-IL-31 antibody has been validated for ELISA, IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse peripheral blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-04
Question
We have tried in the past anti-IL-31 antibody for WB on peripheral blood in the past. I am using mouse, and I plan to use the antibody for IHC-P next. My lab would like examining peripheral blood as well as testis in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?
Verified Customer
Verified customer
Asked: 2018-03-02
Answer
I took a look at the website and datasheets of our anti-IL-31 antibody and it appears that A08339-1 has been validated on mouse in both WB and IHC-P. Thus A08339-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-03-02
Question
Is a blocking peptide available for product anti-IL-31 antibody (A08339-1)?
J. Edwards
Verified customer
Asked: 2017-10-26
Answer
We do provide the blocking peptide for product anti-IL-31 antibody (A08339-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-10-26
Question
I see that the anti-IL-31 antibody A08339-1 works with IHC-P, what is the protocol used to produce the result images on the product page?
R. Parker
Verified customer
Asked: 2017-06-27
Answer
You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-06-27
Question
We are currently using anti-IL-31 antibody A08339-1 for human tissue, and we are content with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2017-05-16
Answer
The anti-IL-31 antibody (A08339-1) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-16
Question
Can you help my question with product A08339-1, anti-IL-31 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Taylor
Verified customer
Asked: 2016-12-20
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08339-1 anti-IL-31 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-12-20
Question
We have been able to see staining in human testis. What should we do? Is anti-IL-31 antibody supposed to stain testis positively?
T. Singh
Verified customer
Asked: 2016-02-08
Answer
From what I have seen in literature testis does express IL31. From what I have seen in Uniprot.org, IL31 is expressed in testis, peripheral blood, among other tissues. Regarding which tissues have IL31 expression, here are a few articles citing expression in various tissues:
Peripheral blood, Pubmed ID: 15184896
Boster Scientific Support
Answered: 2016-02-08
Question
I would like using your anti-IL-31 antibody for cytokine-mediated signaling pathway studies. Has this antibody been tested with western blotting on spleen tissue? We would like to see some validation images before ordering.
A. Gonzalez
Verified customer
Asked: 2015-09-29
Answer
Thank you for your inquiry. This A08339-1 anti-IL-31 antibody is tested on rat thymus tissue, spleen tissue. It is guaranteed to work for ELISA, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-09-29
