Anti-TNIP1 Antibody Picoband™

Product Name

Anti-TNIP1 Antibody Picoband™

View all TNIP1 Antibodies

SKU/Catalog Number

A02299-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TNIP1 Antibody Picoband™ catalog # A02299-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TNIP1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02299-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human TNIP1 recombinant protein (Position: E312-K371).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02299-2 is reactive to TNIP1 in Human, Mouse, Rat

Applications

A02299-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

80-85 kDa

Calculated molecular weight

30392 MW

Background of TNIP1

TNFAIP3-interacting protein 1 is a protein that in humans is encoded by the TNIP1 gene. This gene encodes an A20-binding protein which plays a role in autoimmunity and tissue homeostasis through the regulation of nuclear factor kappa-B activation. Mutations in this gene have been associated with psoriatic arthritis, rheumatoid arthritis, and systemic lupus erythematosus. Multiple transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of TNIP1 using anti-TNIP1 antibody (A02299-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: human T-47D whole cell lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: rat lung tissue lysates,
Lane 8: mouse lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNIP1 antigen affinity purified polyclonal antibody (Catalog # A02299-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNIP1 at approximately 80-85KD. The expected band size for TNIP1 is at 72KD.

Gene/Protein Information For TNIP1 (Source: Uniprot.org, NCBI)

Alternative Names

ABIN-1; HIV-1 Nef-interacting protein; hVAN; KIAA0113nip40-1; Naf1; NAF1TNFAIP3-interacting protein 1; Nef-associated factor 1 SNP; Nef-associated factor 1; Nip40-1; TNFAIP3 interacting protein 1; VANvirion-associated nuclear-shuttling protein; Virion-associated nuclear shuttling protein TNIP1 ABIN-1, NAF1, VAN, nip40-1 TNFAIP3 interacting protein 1 TNFAIP3-interacting protein 1|A20-binding inhibitor of NF-kappa-B activation 1|HIV-1 Nef-interacting protein|Nef-associated factor 1 SNP|virion-associated nuclear shuttling protein

For more info on TNIP1, check out the TNIP1 Infographic

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In this infographic, you will see the following information for TNIP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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