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Product Info Summary
| SKU: | EK1150 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse S100A8/Calgranulin A ELISA Kit PicoKine®
SKU/Catalog Number
EK1150
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse S100A8/Calgranulin A ELISA Kit PicoKine® (96 Tests). Quantitate Mouse S100a8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse S100A8/Calgranulin A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1150)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: P2-E89
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1150 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1150 is reactive to S100a8 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1150 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of S100a8
S100 calcium-binding protein A8 (S100A8), also known as calgranulin A, is a protein that in humans is encoded by the S100A8 gene. It is mapped to chromosome 1q21.3 based on an alignment of the S100A8 sequence with the genomic sequence. The proteins S100A8 and S100A9 form a heterodimer called calprotectin which is a major calcium- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes. This gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. And S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1150-CAP | Anti-Mouse S100a8 Pre-coated 96-well strip microplate | 1 |
| EK1150-ST | Mouse S100a8 Standard | 2 vials, 10 ng/tube |
| EK1150-DA | Mouse S100a8 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.077 | 0.116 | 0.208 | 0.372 | 0.514 | 0.835 | 1.204 | 1.897 |
Data Example Images
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Mouse S100A8 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect S100a8, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 156 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse S100A8/Calgranulin A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 276 | 1214 | 4039 | 294 | 1228 | 4594 |
| Standard deviation | 11.59 | 52.20 | 165.60 | 19.40 | 83.50 | 326.17 |
| CV (%) | 4.2% | 4.3% | 4.1% | 6.6% | 6.8% | 7.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of S100a8 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 276 | 253 | 272 | 279 | 270 | 10.12 | 3.7% |
| Sample 2 | 1214 | 1248 | 1303 | 1250 | 1253 | 31.83 | 2.5% |
| Sample 3 | 4039 | 3861 | 4494 | 4141 | 4133 | 230.86 | 5.5% |
Protein Target Info & Infographic
Gene/Protein Information For S100a8 (Source: Uniprot.Org, NCBI)
Gene Name
S100a8
Full Name
Protein S100-A8
Weight
10295 MW
Superfamily
S-100 family
Alternative Names
Protein S100-A8;Calgranulin-A;Chemotactic cytokine CP-10;Leukocyte L1 complex light chain;Migration inhibitory factor-related protein 8;MRP-8;p8;Pro-inflammatory S100 cytokine;S100 calcium-binding protein A8;S100a8;Caga, Mrp8; S100A8 60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF, P8 S100 calcium binding protein A8 protein S100-A8|calgranulin A|calprotectin L1L subunit|cystic fibrosis |leukocyte L1 complex light chain|migration inhibitory factor-related protein 8|urinary stone protein band A
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on S100a8, check out the S100a8 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for S100a8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse S100A8/Calgranulin A ELISA Kit PicoKine® (EK1150)
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Customer Q&As
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8 Customer Q&As for Mouse S100A8/Calgranulin A ELISA Kit PicoKine®
Question
Could you please provide the detailed sample prep protocol for Cell lysates for EK1150?
Verified customer
Asked: 2021-01-08
Answer
For the Mouse S100A8/Calgranulin A ELISA Kit PicoKine™ (EK1150), please see the following method or buffer for making the lysates. Rinse cells in ice-cold PBS. Scrape cells or trypsinize cells and wash with PBS. Centrifuge at ~3000 x g for 2-3 minutes, remove PBS. The centrifugation force and time can be varied depending on the cell type. Add IP lysis buffer(Boster Cat#AR0107). Sonicate for 10–15 sec to complete cell lysis. Centrifuge at ~10000 x g for 10 minutes. The centrifugation force and time can be varied depending on the cell type. Aspirate supernatant avoiding pellet and upper, lipid-containing phase. Aliquot supernatant and store at -20°C.
Boster Scientific Support
Answered: 2021-01-11
Question
Q: can you recommend the dilution ratio of serum samples for detection of S100a8 in Mouse cell lysates? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-07-19
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the S100a8 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-07-19
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-03-26
Question
Q: What is the optimal O.D. value for S100a8 ELISA kit? I used your S100a8 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain S100a8 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this S100a8 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of S100a8 that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2019-10-31
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-12-08
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-12-08
Question
Q: if the enzyme conjugated S100a8 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the S100a8 antigen?
Verified Customer
Verified customer
Asked: 2018-01-11
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-01-11
Question
Q: which procedure should I follow in order to thaw whole blood sample for S100a8 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2017-11-07
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test S100a8 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2017-11-07
Question
Q: how to analyze ELISA data? I have performed S100a8 level in cell lysates.
W. Turner
Verified customer
Asked: 2016-10-10
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-10-10
