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Product Info Summary
| SKU: | M01139 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IKB alpha NFKBIA Rabbit Monoclonal Antibody
SKU/Catalog Number
M01139
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IKB alpha NFKBIA Rabbit Monoclonal Antibody catalog # M01139. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IKB alpha NFKBIA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01139)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HO-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IKB alpha
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01139 is reactive to NFKBIA in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
36, 39 kDa
Calculated molecular weight
35609 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01139 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30
Positive Control
WB: human Hela whole cell, human Raji whole cell, human PC-3 whole cell, human Jurkat whole cell, rat liver tissue, rat C6 whole cell, mouse liver tissue, mouse Neuro-2a whole cell
IHC: mouse stomach tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IKB alpha using anti-IKB alpha antibody (M01139).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKB alpha antigen affinity purified monoclonal antibody (Catalog # M01139) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKB alpha at approximately 36, 39 kDa. The expected band size for IKB alpha is at 36 kDa.
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Immunohistochemical analysis of paraffin-embedded mouse stomach, using IKB alpha Antibody(M01139)
NFKBIA was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NFKBIA Antibody (M01139)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Immunofluorescent analysis of Hela cells, using IKB alpha Antibody .
Protein Target Info & Infographic
Gene/Protein Information For NFKBIA (Source: Uniprot.org, NCBI)
Gene Name
NFKBIA
Full Name
NF-kappa-B inhibitor alpha
Weight
35609 MW
Superfamily
NF-kappa-B inhibitor family
Alternative Names
NF-kappa-B inhibitor alpha;I-kappa-B-alpha;IkB-alpha;IkappaBalpha;Major histocompatibility complex enhancer-binding protein MAD3;NFKBIA;IKBA, MAD3, NFKBI; NFKBIA EDAID2, IKBA, MAD-3, NFKBI NFKB inhibitor alpha NF-kappa-B inhibitor alpha|I-kappa-B-alpha|IkappaBalpha|ikB-alpha|major histocompatibility complex enhancer-binding protein MAD3|nuclear factor of kappa light chain gene enhancer in B-cells|nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NFKBIA, check out the NFKBIA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NFKBIA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IKB alpha NFKBIA Rabbit Monoclonal Antibody (M01139)
Hello CJ!
M01139 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
N-acetylcysteine reduces oxidative stress, nuclear factor-?B activity and cardiomyocyte apoptosis in heart failure
Downregulation of TNIP1 Expression Leads to Increased Proliferation of Human Keratinocytes and Severer Psoriasis-Like Conditions in an Imiquimod-Induced Mouse Model of Dermatitis
Rumen-derived lipopolysaccharide enhances the expression of lingual antimicrobial peptide in mammary glands of dairy cows fed a high-concentrate diet
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