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Product Info Summary
| SKU: | EKC2295 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
IkappaB-alpha (Phospho-Tyr305) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2295
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The IkappaB-alpha (Phospho-Tyr305) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor IkappaB-alpha (Phospho-Tyr305) protein expression profile in cells. The kit can be used for measuring the relative amounts of IkappaB-alpha (Phospho-Tyr305) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on IkappaB-alpha (Phospho-Tyr305).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
IkappaB-alpha (Phospho-Tyr305) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2295)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2295 is reactive to NFKBIA in Human, Mouse, Rat samples
Validated Sample Types
Cell lines
Application Guarantee
EKC2295 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-IkappaB-alpha Phospho-Tyr305 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-IkappaB-alpha Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from COS7 cells treated with nocodazole 1ug/ml 16h
Protein Target Info & Infographic
Gene/Protein Information For NFKBIA (Source: Uniprot.Org, NCBI)
Gene Name
NFKBIA
Full Name
NF-kappa-B inhibitor alpha
Weight
35609 MW
Superfamily
NF-kappa-B inhibitor family
Alternative Names
I-kappa-B-alpha;IkappaBalpha;IKBA;MAD3;Major histocompatibility complex enhancer-binding protein MAD3;NF-kappaB inhibitor alpha;NFKBI;NFKBIA;RL/IF-1 NFKBIA EDAID2, IKBA, MAD-3, NFKBI NFKB inhibitor alpha NF-kappa-B inhibitor alpha|I-kappa-B-alpha|IkappaBalpha|ikB-alpha|major histocompatibility complex enhancer-binding protein MAD3|nuclear factor of kappa light chain gene enhancer in B-cells|nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on NFKBIA, check out the NFKBIA Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NFKBIA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For IkappaB-alpha (Phospho-Tyr305) Colorimetric Cell-Based ELISA Kit (EKC2295)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for IkappaB-alpha (Phospho-Tyr305) Colorimetric Cell-Based ELISA Kit
Question
Hello Boster: What is the immunogen sequence for this EKC2295 IkappaB-alpha (Phospho-Tyr305) Colorimetric Cell-Based ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-11-26
Answer
The immunogen sequence of EKC2295 IkappaB-alpha (Phospho-Tyr305) Colorimetric Cell-Based ELISA Kit is near the N-terminus, in the general range 15 to 40aa. The definite sequence information is proprietary, thus, we cannot released it, but we can say that it is less than 25aa long.
Boster Scientific Support
Answered: 2018-11-26
Question
I want to ask if this EKC2295 IkappaB-alpha (Phospho-Tyr305) Colorimetric Cell-Based ELISA Kit requires antigen retrieval.
Verified Customer
Verified customer
Asked: 2017-09-14
Answer
We recommend the use of antigen retrieval for the application of EKC2295 IkappaB-alpha (Phospho-Tyr305) Colorimetric Cell-Based ELISA Kit by IHC. And we suggest a standard citrate buffer approach.
Boster Scientific Support
Answered: 2017-09-14
Question
I ordered your product EKC2295 IkappaB-alpha (Phospho-Tyr305) Colorimetric Cell-Based ELISA Kit that I used for western blot and I observed beautiful band for classic form of Ikappa B alpha (37kDa). Even though I have all of this in my western blots, I have an additional band around 47kDa, and I would like to know if I could test a positive control to finally determine if it's an another form of IkappaB or not ?
Verified Customer
Verified customer
Asked: 2013-10-08
Answer
Our product EKC2295 IkappaB-alpha (Phospho-Tyr305) Colorimetric Cell-Based ELISA Kit recognizes both the phosphorylated and non-phosphorylated forms of the serine 32 region of IKB alpha. It means that the band at 37kDa represents the unphosphorylated protein and the band at 47kDa is likely to be due to phosphorylation of serine residues. However, if you would like to test a positive control, we suggest to use HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate.
Boster Scientific Support
Answered: 2013-10-08
