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Product Info Summary
| SKU: | P01139 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-Phospho-IKB alpha (S32) NFKBIA Rabbit Monoclonal Antibody
SKU/Catalog Number
P01139
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-IKB alpha (S32) NFKBIA Rabbit Monoclonal Antibody catalog # P01139. Tested in WB, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-IKB alpha (S32) NFKBIA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01139)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EDG-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-IKB alpha (S32)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P01139 is reactive to NFKBIA in Human
Reconstitution
Observed Molecular Weight
80 kDa
Calculated molecular weight
35609 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01139 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Phospho-IKB alpha (S32) expression in HeLa cell lysate treated with Calyculin A and TNF-a.
Protein Target Info & Infographic
Gene/Protein Information For NFKBIA (Source: Uniprot.org, NCBI)
Gene Name
NFKBIA
Full Name
NF-kappa-B inhibitor alpha
Weight
35609 MW
Superfamily
NF-kappa-B inhibitor family
Alternative Names
NF-kappa-B inhibitor alpha;I-kappa-B-alpha;IkB-alpha;IkappaBalpha;Major histocompatibility complex enhancer-binding protein MAD3;NFKBIA;IKBA, MAD3, NFKBI; NFKBIA EDAID2, IKBA, MAD-3, NFKBI NFKB inhibitor alpha NF-kappa-B inhibitor alpha|I-kappa-B-alpha|IkappaBalpha|ikB-alpha|major histocompatibility complex enhancer-binding protein MAD3|nuclear factor of kappa light chain gene enhancer in B-cells|nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NFKBIA, check out the NFKBIA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NFKBIA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-IKB alpha (S32) NFKBIA Rabbit Monoclonal Antibody (P01139)
Hello CJ!
P01139 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Rumen-derived lipopolysaccharide enhances the expression of lingual antimicrobial peptide in mammary glands of dairy cows fed a high-concentrate diet
Liao, Z., Wang, J., Tan, H., & Wei, L. (2017). Cinnamon extracts exert intrapancreatic cytoprotection against streptozotocin in vivo. Gene, 627, 519-523. doi: 10.1016/j.gene.2017.07.014
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Customer Q&As
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1 Customer Q&As for Anti-Phospho-IKB alpha (S32) NFKBIA Rabbit Monoclonal Antibody
Question
Does P01139 cross react with rat? Keywords: cross reaction, phosphorylation, homology, immunogen
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
Regarding the immunogen sequence, the homology of the human sequence and rat sequence is higher than 90%. In theory, this antibody will cross react with rat. However, since P01139 is a phosphorylated antibody, cross action with rat may not occur if the protein in the rat sample does not have the same phosphorylated epitopes.
Boster Scientific Support
Answered: 2019-08-23
