Click image to see more details
Product Info Summary
| SKU: | A01139-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-IkB-a (Ab-32/36) NFKBIA Antibody
SKU/Catalog Number
A01139-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-IkB-a (Ab-32/36) NFKBIA Antibody (Catalog # A01139-1). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IkB-a (Ab-32/36) NFKBIA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01139-1)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.30~34/34~38 (H-D-S-G-L- D-S -M-K) derived from Human IkB-a.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01139-1 is reactive to NFKBIA in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
50395 MW
Background of IkB-alpha
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.
Mattioli I, et al. (2004)J Immunol; 172(10): 6336-44.
Courtois G, et al. (2003)J Clin Invest; 112(7): 1108-15.
Nair A, et al. (2003) Oncogene; 22(1): 50-8.
Fan C, et al. (2002)J Cell Sci; 115(Pt 24): 4843-53.
Schubert SY, et al. (2002)FASEB J; 16(14): 1931-3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01139-1 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 39kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NFKBIA using anti-NFKBIA antibody (A01139-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFKBIA antigen affinity purified polyclonal antibody (Catalog # A01139-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for NFKBIA.
Click image to see more details
Immunofluorescence staining of methanol-fixed Hela cells using IkB-a(Ab-32/36) Antibody #A01139-1.
Click image to see more details
Figure 2. IHC analysis of NFKBIA using anti-NFKBIA antibody (A01139-1).
NFKBIA was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NFKBIA Antibody (A01139-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For NFKBIA (Source: Uniprot.org, NCBI)
Gene Name
NFKBIA
Full Name
NF-kappa-B inhibitor alpha
Weight
50395 MW
Superfamily
NF-kappa-B inhibitor family
Alternative Names
Aldehyde dehydrogenase, dimeric NADP-preferring;1.2.1.5;ALDHIII;Aldehyde dehydrogenase 3;Aldehyde dehydrogenase family 3 member A1;ALDH3A1;ALDH3; NFKBIA EDAID2, IKBA, MAD-3, NFKBI NFKB inhibitor alpha NF-kappa-B inhibitor alpha|I-kappa-B-alpha|IkappaBalpha|ikB-alpha|major histocompatibility complex enhancer-binding protein MAD3|nuclear factor of kappa light chain gene enhancer in B-cells|nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NFKBIA, check out the NFKBIA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NFKBIA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IkB-a (Ab-32/36) NFKBIA Antibody (A01139-1)
Hello CJ!
A01139-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Downregulation of TNIP1 Expression Leads to Increased Proliferation of Human Keratinocytes and Severer Psoriasis-Like Conditions in an Imiquimod-Induced Mouse Model of Dermatitis
Rumen-derived lipopolysaccharide enhances the expression of lingual antimicrobial peptide in mammary glands of dairy cows fed a high-concentrate diet
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-IkB-a (Ab-32/36) NFKBIA Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-IkB-a (Ab-32/36) NFKBIA Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
