Click image to see more details
Product Info Summary
| SKU: | M00393-5 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Mouse |
| Host: | Rat |
| Application: | IHC-P |
Customers Who Bought This Also Bought
Product info
Product Name
Anti- IFN Gamma Monoclonal Antibody
SKU/Catalog Number
M00393-5
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti- IFN Gamma Monoclonal Antibody catalog # M00393-5. Tested in IHC-P applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti- IFN Gamma Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00393-5)
Host
Rat
Contents
PBS, pH 7.0. Contains no stabilizers or preservatives
Clonality
Monoclonal
Clone Number
AFII-9
Isotype
Rat IgG1, κ
Immunogen
Recombinant mouse IFNγ
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins
Reactive Species
M00393-5 is reactive to Ifng in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Calculated molecular weight
462529 MW
Background of IFN-gamma
Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00393-5 is guaranteed for IHC-P Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse
Positive Control
IHC: mouse lymphaden tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of IFN Gamma using anti-IFN Gamma antibody (M00393-5).
IFN Gamma was detected in paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml rat anti-IFN Gamma Antibody (M00393-5) overnight at 4°C. Biotinylated goat anti-rat IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Ifng (Source: Uniprot.org, NCBI)
Gene Name
Ifng
Full Name
Interferon gamma
Weight
462529 MW
Superfamily
type II (or gamma) interferon family
Alternative Names
Polycystin-1;Autosomal dominant polycystic kidney disease 1 protein;PKD1; IFNG IFG, IFI, IMD69 interferon gamma interferon gamma|IFN-gamma|immune interferon
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ifng, check out the Ifng Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ifng: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti- IFN Gamma Monoclonal Antibody (M00393-5)
Hello CJ!
M00393-5 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Combination of mesenchymal stem cells and FK506 prolongs heart allograft survival by inhibiting TBK1/IRF3-regulated-IFN-γ production
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti- IFN Gamma Monoclonal Antibody ?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti- IFN Gamma Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
