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Product Info Summary
| SKU: | EK0373 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
Product info
Product Name
Human IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine®
SKU/Catalog Number
EK0373
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine® (96 Tests). Quantitate Human IFNG in cell culture supernatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0373)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: Q24-Q166
Sensitivity
<2 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0373 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0373 is reactive to IFNG in Human samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK0373 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IFN-gamma
Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0373-CAP | Anti-Human IFNG Pre-coated 96-well strip microplate | 1 |
| EK0373-ST | Human IFNG Standard | 2 vials, 10 ng/tube |
| EK0373-DA | Human IFNG Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.023 | 0.073 | 0.102 | 0.185 | 0.357 | 0.612 | 1.138 | 1.901 |
Data Example Images
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Human IFN gamma PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IFN-gamma, Dilution ratio of 1:1, concentration in serum is less than the lowest standard, 15.6 pg/ml..
Some articles we found to cite concentrations of IFN-gamma in samples: 25722655 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 34 | 150 | 469 | 33 | 139 | 437 |
| Standard deviation | 1.73 | 8.7 | 29.54 | 1.91 | 9.73 | 31.9 |
| CV (%) | 7.3% | 5.8% | 7% | 5.8% | 7% | 7.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IFN-gamma in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 34 | 38 | 37 | 36 | 36 | 1.47 | 4% |
| Sample 2 | 150 | 152 | 164 | 166 | 158 | 7.07 | 4.4% |
| Sample 3 | 469 | 446 | 458 | 468 | 460 | 9.28 | 2% |
Protein Target Info & Infographic
Gene/Protein Information For IFNG (Source: Uniprot.Org, NCBI)
Gene Name
IFNG
Full Name
Interferon gamma
Weight
19348 MW
Superfamily
type II (or gamma) interferon family
Alternative Names
Interferon gamma;IFN-gamma;Immune interferon;IFNG; IFNG IFG, IFI, IMD69 interferon gamma interferon gamma|IFN-gamma|immune interferon
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IFNG, check out the IFNG Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IFNG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine® (EK0373)
Hello CJ!
EK0373 has been cited in 82 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Epigenetic suppression of the antitumor cytotoxicity of NK cells by histone deacetylase inhibitor valproic acid
Therapeutic effects of ciprofloxacin/bushenhuazhuo combination on chronic prostatitis
Cytokine Pattern is Affected by Training Intensity in Women Futsal Players
Inflammatory response is modulated by lincRNACox2 via the NF‑κB pathway in macrophages infected by Mycobacterium tuberculosis
Construction of chimeric antigen receptor‑modified T cells targeting EpCAM and assessment of their anti‑tumor effect on cancer cells
‘Psoriasis 1’ reduces psoriasis‑like skin inflammation by inhibiting the VDR‑mediated nuclear NF‑κB and STAT signaling pathways
γδTCR immunoglobulin constant region domain exchange in human αβTCRs improves TCR pairing without altering TCR gene-modified T cell function
Increased soluble and membrane-bound PD-L1 contributes to immune regulation and disease progression in patients with tuberculous pleural effusion
Capsaicin induces immunogenic cell death in human osteosarcoma cells
Influence of Immunogenicity of Allogeneic Bone Marrow Mesenchymal Stem Cells on Bone Tissue Engineering:
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Customer Reviews
Have you used Human IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine®?
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1 Reviews For Human IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine®
0
Very Easy To Work With And Great Results With Least Standard Error--Akshay Jain, University of Missouri, Kansas City, Pharmaceutical Sciences, Research Assistant
Excellent
Source: Biocompare.com
| Applications | ELISA (Inflammatory cytokine assay from plasma) |
|---|---|
| Sample | Rat plasma |
| Detection | -- |
"I used this assay kit for detection of the cytokines in the plasma samples. Only 100 uL of sample yielded absolutely flawless results. Plasma samples showed variable cytokine expression among independent groups which demonstrates the accuracy of this kit. Straightforward protocol. Very easy to perform. Highly recommended product for precise cytokine assay analysis." (Customer’s Tip: "Keep working area clean. Prepare necessary dilution of the sample before starting the procedure.")
Akshay Jain
Verified customer
Submitted 2017-08-21
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
10 Customer Q&As for Human IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine®
Question
Could you please let us know exactly which subtypes of interferon are detected by the kit?
Verified customer
Asked: 2022-08-04
Answer
Thank you for your inquiry. The kit detects IFN Gamma. Immunogen of both the capture and detection antibodies is 24-166aa.
Boster Scientific Support
Answered: 2022-08-04
Question
Which subtypes of interferon are detected by EK0373?
Verified customer
Asked: 2022-08-03
Answer
The Human IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine® (EK0373) detects IFN Gamma.
Boster Scientific Support
Answered: 2022-08-04
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis IFN-gamma using the EK0373 Human IFN-gamma Picokine® ELISA Kit?
M. Liu
Verified customer
Asked: 2020-11-24
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2020-11-24
Question
Is EK0373 suitable for co-culture Target Cells and Effector Cells and ELISA with cell culture fluid?
Verified customer
Asked: 2020-11-12
Answer
Our lab hasn't worked on co-culture cell samples using the Human IFN Gamma ELISA Kit PicoKine™ (EK0373). Please run a pilot test to see if the kits could work on the specific samples.
Boster Scientific Support
Answered: 2020-11-16
Question
Q: which procedure should I follow in order to thaw whole blood sample for IFN-gamma ELISA after freezing?
Y. Sunder
Verified customer
Asked: 2020-01-03
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IFN-gamma for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-01-03
Question
What is the normal serum range of EK0373?
Verified customer
Asked: 2019-04-08
Answer
Please see the normal serum range for the Human IFN Gamma ELISA Kit PicoKine™ (EK0373) below; IFN-gamma ( catlog no. EK0373) 0-24pg/mL
Boster Scientific Support
Answered: 2019-04-09
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-02-18
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-02-18
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
B. Hill
Verified customer
Asked: 2017-05-28
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2017-05-28
Question
Q: Can IFN-gamma ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
T. Taylor
Verified customer
Asked: 2016-09-03
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2016-09-03
Question
Dear Support: I purchased your product EK0373, can the reconstituted standard at 25pg/ml be stored at 2-8C until the expiration and be reused? Can the PBS substituted with PBST as the wash buffer?
Verified Customer
Verified customer
Asked: 2014-06-19
Answer
We do not recommend storing reconstituted standard for these EK0373 antibody because it is not stable and it may not work after storage. Yes, the wash buffer can be substituted with PBS with 0.05% Tween.
Boster Scientific Support
Answered: 2014-06-19
