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Product Info Summary
| SKU: | EK0952-RB |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rabbit |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rabbit Endothelin 1/EDN1 ELISA Kit PicoKine®
View all Endothelin-1 ELISA kits
SKU/Catalog Number
EK0952-RB
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rabbit Endothelin 1/EDN1 ELISA Kit PicoKine® (96 Tests). Quantitate Rabbit EDN1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rabbit Endothelin 1/EDN1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0952-RB)
Clonality of Antibodies
See Datasheet for details
Standard Protein
A peptide reflecting the target protein in rabbit. Sequence: C53-W73
Sensitivity
<0.5 pg/ml
Assay Range
3.9 pg/ml - 250 pg/ml
Standard Dilution Instructions
See datasheet of EK0952-RB for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0952-RB is reactive to EDN1 in Rabbit samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK0952-RB is guaranteed for ELISA in Rabbit by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Endothelin-1
The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of rabbit endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure (hypertension) and heart disease.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0952-RB-CAP | Anti-Rabbit EDN1 Pre-coated 96-well strip microplate | 1 |
| EK0952-RB-ST | Rabbit EDN1 Standard | 2 vials, 1 ng/tube |
| EK0952-RB-DA | Rabbit EDN1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 3.9 | 7.8 | 15.6 | 31.2 | 62.5 | 125 | 250 |
| O.D. | 0.068 | 0.093 | 0.121 | 0.193 | 0.354 | 0.715 | 1.559 | 2.457 |
Data Example Images
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Rabbit Endothelin PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rabbit Endothelin 1/EDN1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 26.2 | 88.7 | 144 | 25.4 | 91.5 | 151 |
| Standard deviation | 1.18 | 7.94 | 6.81 | 1.73 | 7.05 | 11.93 |
| CV (%) | 4.5 | 5.8 | 5.7 | 6.8 | 7.7 | 7.9 |
Protein Target Info & Infographic
Gene/Protein Information For EDN1 (Source: Uniprot.Org, NCBI)
Gene Name
EDN1
Full Name
Endothelin-1
Weight
23135 MW
Superfamily
endothelin/sarafotoxin family
Alternative Names
Endothelin-1;ET-1;Preproendothelin-1;PPET1;Edn1; EDN1 ARCND3, ET1, HDLCQ7, PPET1, QME endothelin 1 endothelin-1|preproendothelin-1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on EDN1, check out the EDN1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for EDN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rabbit Endothelin 1/EDN1 ELISA Kit PicoKine® (EK0952-RB)
Hello CJ!
EK0952-RB has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ameliorative effects of the traditional Chinese medicine formula Qing-Mai-Yin on arteriosclerosis obliterans in a rabbit model
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Rabbit Endothelin 1/EDN1 ELISA Kit PicoKine®
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
P. Dayal
Verified customer
Asked: 2020-11-16
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-11-16
Question
Q: how do I analyze ELISA data? I measured Endothelin-1 level in serum.
Verified Customer
Verified customer
Asked: 2020-10-05
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-10-05
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
W. Robinson
Verified customer
Asked: 2018-10-24
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-10-24
Question
Q: Can Endothelin-1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-10-15
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-10-15
Question
Q: how to thaw whole blood sample for Endothelin-1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-08-23
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Endothelin-1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-08-23
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Endothelin-1 in Rabbit serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-08-01
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Endothelin-1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-08-01
Question
Q: is it okay to use citrate plasma as samples in Rabbit Endothelin-1 Picokine® ELISA Kit (Catalog # EK0952-RB)?
Z. Campbell
Verified customer
Asked: 2017-09-08
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Endothelin-1 causing degradation of its protein structure. Endothelin-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Endothelin-1 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-09-08
