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Product Info Summary
| SKU: | A00393-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA |
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Product info
Product Name
Anti-Interferon gamma IFNG Antibody
SKU/Catalog Number
A00393-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interferon gamma IFNG Antibody catalog # A00393-1. Tested in ELISA applications. This antibody reacts with Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interferon gamma IFNG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00393-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat Interferon gamma recombinant protein (Position: M1-C156).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00393-1 is reactive to IFNG in Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
17628 MW
Background of IFN-gamma
Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00393-1 is guaranteed for ELISA Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA Capture (Matched Antibody Pair), 2-8μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Sandwich ELISA - Recombinant rat Interferon gamma protein standard curve.
Use in combination with reagents from Rat Interferon gamma ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0374).
Protein Target Info & Infographic
Gene/Protein Information For IFNG (Source: Uniprot.org, NCBI)
Gene Name
IFNG
Full Name
Interferon gamma
Weight
17628 MW
Superfamily
type II (or gamma) interferon family
Alternative Names
Interferon gamma; IFN-gamma; IFNG IFNG IFG, IFI, IMD69 interferon gamma interferon gamma|IFN-gamma|immune interferon
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IFNG, check out the IFNG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IFNG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Interferon gamma IFNG Antibody (A00393-1)
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Customer Q&As
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15 Customer Q&As for Anti-Interferon gamma IFNG Antibody
Question
My boss were well pleased with the WB result of your anti-Interferon gamma antibody. However we have observed positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IFNG supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
According to literature, blood does express IFNG. Generally IFNG expresses in secreted. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-04-21
Question
Is this A00393-1 anti-Interferon gamma antibody reactive to the isotypes of IFNG?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
The immunogen of A00393-1 anti-Interferon gamma antibody is E. coli-derived rat Interferon gamma recombinant protein (Position: M1-C156). . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-03
Question
We have observed staining in rat leukocyte. What should we do? Is anti-Interferon gamma antibody supposed to stain leukocyte positively?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
Based on literature leukocyte does express IFNG. Based on Uniprot.org, IFNG is expressed in leukocyte, blood, among other tissues. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-06
Question
I was wanting to use your anti-Interferon gamma antibody for ELISA for rat leukocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat leukocyte identification?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
You can see on the product datasheet, A00393-1 anti-Interferon gamma antibody has been tested for ELISA on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-01
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-Interferon gamma antibody A00393-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-27
Question
I see that the anti-Interferon gamma antibody A00393-1 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-14
Question
Would anti-Interferon gamma antibody A00393-1 work for ELISA with leukocyte?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
According to the expression profile of leukocyte, IFNG is highly expressed in leukocyte. So, it is likely that anti-Interferon gamma antibody A00393-1 will work for ELISA with leukocyte.
Boster Scientific Support
Answered: 2019-07-22
Question
Would A00393-1 anti-Interferon gamma antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
It shows on the product datasheet, A00393-1 anti-Interferon gamma antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-01
Question
Here is the WB image, lot number and protocol we used for leukocyte using anti-Interferon gamma antibody A00393-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-27
Question
We are currently using anti-Interferon gamma antibody A00393-1 for rat tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
The anti-Interferon gamma antibody (A00393-1) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-20
Question
I am interested in A00393-1 kit but I would like to know the approximate concentration range and the isotype of this antibody.
Verified Customer
Verified customer
Asked: 2019-05-10
Answer
The A00393-1 antibody kit contain 100ug per vial, so we would recommend to reconstitute this in 200ul of sterile distilled water which gives a stock concentration of 0.5mg/ml. In regards to the isotype of this antibody it is polyclonal thus, it contains a mixture of all antibody isotypes but the main isotype of polyclonal will be IgG.
Boster Scientific Support
Answered: 2019-05-10
Question
Is a blocking peptide available for product anti-Interferon gamma antibody (A00393-1)?
C. Gonzalez
Verified customer
Asked: 2018-10-26
Answer
We do provide the blocking peptide for product anti-Interferon gamma antibody (A00393-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-10-26
Question
Can you help my question with product A00393-1, anti-Interferon gamma antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Thomas
Verified customer
Asked: 2018-10-04
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00393-1 anti-Interferon gamma antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-04
Question
Do you have a BSA free version of anti-Interferon gamma antibody A00393-1 available?
Verified Customer
Verified customer
Asked: 2017-08-09
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Interferon gamma antibody A00393-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-08-09
Question
We want to test anti-Interferon gamma antibody A00393-1 on rat leukocyte for research purposes, then I may be interested in using anti-Interferon gamma antibody A00393-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Evans
Verified customer
Asked: 2016-11-10
Answer
The products we sell, including anti-Interferon gamma antibody A00393-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-11-10
