Click image to see more details
Product Info Summary
| SKU: | A00393-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Interferon gamma/IFNG Antibody Picoband®
SKU/Catalog Number
A00393-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interferon gamma/IFNG Antibody Picoband® catalog # A00393-3. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interferon gamma/IFNG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00393-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Interferon gamma recombinant protein (Position: Q24-G161).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00393-3 is reactive to IFNG in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
19 kDa
Calculated molecular weight
19,348 MW
Background of IFN-gamma
IInterferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00393-3 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
ELISA(Cap), 1-5μg/ml
Positive Control
WB: human placenta tissue, rat spleen tissue, mouse spleen tissue, mouse thymus tissue
IHC: human tonsil tissue, mouse colon tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of IFNG using anti-IFNG antibody (A00393-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: mouse spleen tissue lysates,
Lane 4: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNG antigen affinity purified polyclonal antibody (Catalog # A00393-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFNG at approximately 19 kDa. The expected band size for IFNG is at 19 kDa.
Click image to see more details
Figure 2. IHC analysis of IFNG using anti-IFNG antibody (A00393-3).
IFNG was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IFNG Antibody (A00393-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of IFNG using anti-IFNG antibody (A00393-3).
IFNG was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IFNG Antibody (A00393-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For IFNG (Source: Uniprot.org, NCBI)
Gene Name
IFNG
Full Name
Interferon gamma
Weight
19,348 MW
Superfamily
type II (or gamma) interferon family
Alternative Names
Interferon gamma; IFN-gamma; Immune interferon; IFNG IFNG IFG, IFI, IMD69 interferon gamma interferon gamma|IFN-gamma|immune interferon
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IFNG, check out the IFNG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IFNG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Interferon gamma/IFNG Antibody Picoband® (A00393-3)
Hello CJ!
A00393-3 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The expression and significance of IL-6, IFN-γ, SM22α, and MMP-2 in rat model of aortic dissection.
Human interleukin-10 gene inhibits acute rejection by triggering apoptosis in allograft vascular transplantation
Anti‐arthritis activity of cationic materials
Bu-Shen-Fang-Chuan formula attenuates T-lymphocytes recruitment in the lung of rats with COPD through suppressing CXCL9/CXCL10/CXCL11-CXCR3 axis
Quercetin inhibiting the PD-1/PD-L1 interaction for immune-enhancing cancer chemopreventive agent
Protective effect of carnosine after chronic cerebral hypoperfusion possibly through suppressing astrocyte activation
Somatostatin Improved B Cells Mature in Macaques during Intestinal Ischemia-Reperfusion
Somatostatin Inhibits the Production of Interferon-? by Intestinal Epithelial Cells During Intestinal Ischemia?Reperfusion in Macaques
Yan X, Wang D, Liang F, Fu L, Guo C. Hum Vaccin Immunother. 2014;10(12):3491-8. Doi: 10.4161/Hv.36084. Hpv16L1-Attenuated Shigella Recombinant Vaccine Induced Strong Vaginal And Systemic Immune Responses In Guinea Pig Model.
Li Xl, Dou Yc, Liu Y, Shi Cw, Cao Ll, Zhang Xq, Zhu J, Duan Rs. Cell Immunol. 2011;271(2):455-61. Doi: 10.1016/J.Cellimm.2011.08.015. Epub 2011 Aug 23. Atorvastatin Ameliorates Experimental Autoimmune Neuritis By Decreased Th1/Th17 Cytokines And U...
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Interferon gamma/IFNG Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Interferon gamma/IFNG Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-Interferon gamma/IFNG Antibody Picoband®
Question
We have been able to see staining in rat blood. Do you have any suggestions? Is anti-Interferon gamma/IFNG antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
According to literature blood does express IFNG. According to Uniprot.org, IFNG is expressed in leukocyte, blood, among other tissues. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-04-16
Question
Will A00393-3 anti-Interferon gamma/IFNG antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
It shows on the product datasheet, A00393-3 anti-Interferon gamma/IFNG antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-17
Question
Is this A00393-3 anti-Interferon gamma/IFNG antibody reactive to the isotypes of IFNG?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
The immunogen of A00393-3 anti-Interferon gamma/IFNG antibody is E. coli-derived human Interferon gamma recombinant protein (Position: Q24-G161). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-17
Question
Would anti-Interferon gamma/IFNG antibody A00393-3 work for WB with leukocyte?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
According to the expression profile of leukocyte, IFNG is highly expressed in leukocyte. So, it is likely that anti-Interferon gamma/IFNG antibody A00393-3 will work for WB with leukocyte.
Boster Scientific Support
Answered: 2019-12-11
Question
I am interested in using your anti-Interferon gamma/IFNG antibody for regulation of growth studies. Has this antibody been tested with western blotting on placenta tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
Thanks for your inquiry. This A00393-3 anti-Interferon gamma/IFNG antibody is validated on human sarcoma tissue, hela whole cell lysates, placenta tissue, rat lung tissue, brain tissue, mouse ovary tissue, cancer tissue, tonsil tissue, testis tissue. It is guaranteed to work for IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-19
Question
I see that the anti-Interferon gamma/IFNG antibody A00393-3 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-23
Question
Can you help my question with product A00393-3, anti-Interferon gamma/IFNG antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00393-3 anti-Interferon gamma/IFNG antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-12
Question
We need to test anti-Interferon gamma/IFNG antibody A00393-3 on rat leukocyte for research purposes, then I may be interested in using anti-Interferon gamma/IFNG antibody A00393-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Anderson
Verified customer
Asked: 2018-04-24
Answer
The products we sell, including anti-Interferon gamma/IFNG antibody A00393-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-04-24
Question
I was wanting to use your anti-Interferon gamma/IFNG antibody for WB for rat leukocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat leukocyte identification?
J. Anderson
Verified customer
Asked: 2018-04-19
Answer
As indicated on the product datasheet, A00393-3 anti-Interferon gamma/IFNG antibody has been validated for IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-19
Question
Our lab were happy with the WB result of your anti-Interferon gamma/IFNG antibody. However we have observed positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IFNG supposed to be expressed?
G. Li
Verified customer
Asked: 2018-02-28
Answer
According to literature, blood does express IFNG. Generally IFNG expresses in secreted. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-02-28
Question
Is a blocking peptide available for product anti-Interferon gamma/IFNG antibody (A00393-3)?
Verified Customer
Verified customer
Asked: 2017-12-27
Answer
We do provide the blocking peptide for product anti-Interferon gamma/IFNG antibody (A00393-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-12-27
Question
Do you have a BSA free version of anti-Interferon gamma/IFNG antibody A00393-3 available?
C. Zhang
Verified customer
Asked: 2016-02-26
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Interferon gamma/IFNG antibody A00393-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-02-26
Question
We are currently using anti-Interferon gamma/IFNG antibody A00393-3 for rat tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
C. Kulkarni
Verified customer
Asked: 2015-11-09
Answer
The anti-Interferon gamma/IFNG antibody (A00393-3) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-11-09
Question
See below the WB image, lot number and protocol we used for leukocyte using anti-Interferon gamma/IFNG antibody A00393-3. Please let me know if you require anything else.
K. Brown
Verified customer
Asked: 2015-10-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-10-16
Question
We purchased anti-Interferon gamma/IFNG antibody for IHC-P on blood in a previous experiment. I am using mouse, and We are going to use the antibody for WB next. My lab would like examining blood as well as leukocyte in our next experiment. Could give a recommendation on which antibody would work the best for WB?
R. Li
Verified customer
Asked: 2015-08-19
Answer
I have checked the website and datasheets of our anti-Interferon gamma/IFNG antibody and I see that A00393-3 has been tested on mouse in both IHC-P and WB. Thus A00393-3 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-08-19
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-Interferon gamma/IFNG antibody A00393-3. Let me know if you need anything else.
M. Patel
Verified customer
Asked: 2013-02-12
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-02-12
