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Product Info Summary
| SKU: | PA1112 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-IFITM1 Antibody Picoband®
SKU/Catalog Number
PA1112
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IFITM1 Antibody catalog # PA1112. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IFITM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1112)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human IFITM1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1112 is reactive to IFITM1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
70-74 kDa
Calculated molecular weight
13964 MW
Background of IFITM1
Interferon-induced Transmembrane Protein 1 (IFITM1), also called Interferon-induced Protein 17 (IFI17). IFITM1 activity is required for primordial germ cells (PGCs) transit from the mesoderm into the endoderm, and that it appears to act via a repulsive mechanism, such that PGCs avoid Ifitm1-expressing tissues. It is mapped to Chr.11 and belongs to the family of interferon-induced transmembrane proteins (Ifitm/mil/fragilis), which encodes cell surface proteins that may modulate cell adhesion and influence cell differentiation. Interferon-inducible membrane proteins of approximately 17 kDa have been suggested to play a role in the antiproliferative activity of interferons based on their pattern of induction in interferon-sensitive and -resistant cell lines and the ability of a membrane fraction enriched in 17-kDa proteins to inhibit cell growth.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1112 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: SW620 Cell, CEM Cell
IHC: Human Intestinal Cancer Tissue
Validation Images & Assay Conditions
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Anti-IFITM1 antibody, PA1112, Western blotting
Lane 1: SW620 Cell Lysate
Lane 2: CEM Cell Lysate
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Anti-IFITM1 antibody, PA1112, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For IFITM1 (Source: Uniprot.org, NCBI)
Gene Name
IFITM1
Full Name
Interferon-induced transmembrane protein 1
Weight
13964 MW
Superfamily
CD225/Dispanin family
Alternative Names
Interferon-induced transmembrane protein 1;Dispanin subfamily A member 2a;DSPA2a;Interferon-induced protein 17;Interferon-inducible protein 9-27;Leu-13 antigen;CD225;IFITM1;CD225, IFI17; IFITM1 9-27, CD225, DSPA2a, IFI17, LEU13 interferon induced transmembrane protein 1 interferon-induced transmembrane protein 1|dispanin subfamily A member 2a|interferon-induced protein 17|interferon-inducible protein 9-27|leu-13
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IFITM1, check out the IFITM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IFITM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IFITM1 Antibody Picoband® (PA1112)
Hello CJ!
PA1112 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yang G, Xu Y, Chen X, Hu G. Oncogene. 2007 Jan 25;26(4):594-603. Epub 2006 Jul 17. Ifitm1 Plays An Essential Role In The Antiproliferative Action Of Interferon-Gamma.
Xu Y, Yang G, Hu G. Acta Biochim Biophys Sin (Shanghai). 2009 Jun;41(6):488-94. Binding Of Ifitm1 Enhances The Inhibiting Effect Of Caveolin-1 On Erk Activation.
Ma Y, Zhang G, Fu X, Xia O, Zhan C, Li L, Wang Z, Wu B. Oncol Rep. 2010 Jun;23(6):1569-76. Wnt Signaling May Be Activated In A Subset Of Peutz-Jeghers Syndrome Polyps Closely Correlating To Lkb1 Expression.
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-IFITM1 Antibody Picoband®
Question
I have a question about product PA1112, anti-IFITM1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1112 anti-IFITM1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-25
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-IFITM1 antibody PA1112. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-09
Question
I was wanting to use your anti-IFITM1 antibody for IHC for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?
Verified Customer
Verified customer
Asked: 2019-04-29
Answer
It shows on the product datasheet, PA1112 anti-IFITM1 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-29
Question
Is this PA1112 anti-IFITM1 antibody reactive to the isotypes of IFITM1?
Verified Customer
Verified customer
Asked: 2019-01-31
Answer
The immunogen of PA1112 anti-IFITM1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human IFITM1(1-17aa MHKEEHEVAVLGPPPST). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-31
Question
We are currently using anti-IFITM1 antibody PA1112 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-06
Answer
The anti-IFITM1 antibody (PA1112) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-06
