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Product Info Summary
| SKU: | H01857 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-beta Tubulin Rabbit Monoclonal Antibody, HRP Conjugated
View all beta-III Tubulin Antibodies
SKU/Catalog Number
H01857
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-beta Tubulin Rabbit Monoclonal Antibody, HRP Conjugated catalog # H01857. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-beta Tubulin Rabbit Monoclonal Antibody, HRP Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # H01857)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DF-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human beta Tubulin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
H01857 is reactive to TUBB3 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
68 kDa
Calculated molecular weight
50433 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
H01857 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:20000
Positive Control
WB: Jurkat cell, Human kidney, 3T3 cell, Mouse brain, C6 cell, Rat heart (H01857)
Validation Images & Assay Conditions
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Western blot analysis of beta Tubulin expression in (1) Jurkat cell lysate, (2) Human kidney lysate, (3) 3T3 cell lysate, (4) Mouse brain lysate, (5) C6 cell lysate, (6) Rat heart lysate (H01857).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBB3 monoclonal antibody (Catalog # H01857) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBB3
Protein Target Info & Infographic
Gene/Protein Information For TUBB3 (Source: Uniprot.org, NCBI)
Gene Name
TUBB3
Full Name
Tubulin beta-3 chain
Weight
50433 MW
Superfamily
tubulin family
Alternative Names
Tubulin beta-3 chain;Tubulin beta-4 chain;Tubulin beta-III;TUBB3;TUBB4; TUBB3 CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4 tubulin beta 3 class III tubulin beta-3 chain|class III beta-tubulin|tubulin beta-4 chain|tubulin beta-III|tubulin, beta 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TUBB3, check out the TUBB3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TUBB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-beta Tubulin Rabbit Monoclonal Antibody, HRP Conjugated (H01857)
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