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Product Info Summary
| SKU: | M00254-3 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Human Ki67 Antibody
View all Ki67/MKI67 Antibodies
SKU/Catalog Number
M00254-3
Size
100ul
Description
Boster Bio Anti-Human Ki67 Antibody catalog # M00254-3. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human Ki67 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00254-3)
Host
Rabbit
Contents
Serum with 5mM NaN3
Clonality
Polyclonal
Immunogen
Recombinant human construct containing amino acids 1,111-1,490 expressed in and purified from E. coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00254-3 is reactive to MKI67 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
358694 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00254-3 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-5,000. IF 1:2,000-5,000, IHC 1:1,000
Validation Images & Assay Conditions
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Western blot analysis of equal amounts of cell lysates using rabbit pAb to Ki-67 M00254-3, dilution 1:10,000, (green): [1] protein standard (red), [2] HeLa cells, [3] HEK293 cells. Strong double bands above 250kDa correspond to the two major Ki-67 isoforms of molecular weight 345kDa and 395kDa. Smaller proteolytic fragments of these isoforms are also detected on the blot.
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Immunofluorescent analysis of HeLa cells stained with rabbit pAb to Ki-67 M00254-3, dilution 1:5,000 in red, and mouse monoclonal antibody to fibrillarin, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. The Ki-67 protein accumulates
Protein Target Info & Infographic
Gene/Protein Information For MKI67 (Source: Uniprot.org, NCBI)
Gene Name
MKI67
Full Name
Proliferation marker protein Ki-67
Weight
358694 MW
Alternative Names
Proliferation marker protein Ki-67 ;Antigen identified by monoclonal antibody Ki-67 ;Antigen KI-67 ;Antigen Ki67 ;MKI67 ; MKI67 KIA, MIB-, MIB-1, PPP1R105 marker of proliferation Ki-67 proliferation marker protein Ki-67| Ki67| by Ki-67|proliferation-related Ki-67 |protein phosphatase 1, regulatory subunit 105
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MKI67, check out the MKI67 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MKI67: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human Ki67 Antibody (M00254-3)
Hello CJ!
M00254-3 has been cited in 16 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Improved neovascularization and wound repair by targeting human basic fibroblast growth factor (bFGF) to fibrin
Xiaozhu Tian,Rong Li,Yiming Jiang,Fei Zhao,Zhengsheng Yu,Yiqing Wang,Zixing Dong,Pu Liu,Xiangkai Li,Bifidobacterium breve ATCC15700 pretreatment prevents alcoholic liver disease through modulating gut microbiota in mice exposed to chronic alcohol intake,J
Species: Mouse
M00254-3 usage in article: APP:IHC, SAMPLE:LIVER TISSUE, DILUTION:NA
Xu X,Li L,Li X,Tao D,Zhang P,Gong J.Aptamer-protamine-siRNA nanoparticles in targeted therapy of ErbB3 positive breast cancer cells.Int J Pharm.2020 Nov 30;590:119963.doi:10.1016/j.ijpharm.2020.119963.Epub 2020 Oct 9.PMID:33039492.
Species: Human,Mouse
M00254-3 usage in article: APP:WB, SAMPLE:MCF-7 CELLS, DILUTION:NA
Yang Z,Zhang J,Lu D,Sun Y, Zhao X,Wang X,Zhou W,He Q,Jiang Z.Hsa_circ_0137008 suppresses the malignant phenotype in colorectal cancer by acting as a microRNA-338-5p sponge. Cancer Cell Int.2020 Mar 4;20:67. doi:10.1186/s1293 5-020-1150-1.PMID:32158357;PMC
Species: Human
M00254-3 usage in article: APP:WB, SAMPLE:SW480 AND HCT116 CELLS, DILUTION:NA
Zhang L, Sun D, Zhang J, Tian Y. Circ-UBR1 facilitates proliferation, metastasis, and inhibits apoptosis in breast cancer by regulating the miR-1299/CCND1 axis. Life Sci. 2020 Nov 28;266:118829. doi: 10.1016/j.lfs.2020.118829. Epub ahead of print. PMID: 3
Species: Human, Mouse
M00254-3 usage in article: APP:IHC, SAMPLE:SUBUTANEOUS TUMOR TISSUE, DILUTION:1:400
Deletion of %u03B2-Arrestin2 in Mice Limited Pancreatic %u03B2-Cell Expansion under Metabolic Stress through Activation of the JNK Pathway
KDM4B and KDM4A promote endometrial cancer progression by regulating androgen receptor, c-myc, and p27kip1
WX-132-18B, a novel microtubule inhibitor, exhibits promising anti-tumor effects
Nek7 is overexpressed in hepatocellular carcinoma and promotes hepatocellular carcinoma cell proliferation in vitro and in vivo
Soy isoflavone extracts stimulate the growth of nude mouse xenografts bearing estrogen-dependent human breast cancer cells (MCF-7)
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