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Product Info Summary
| SKU: | PB9026 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Ki67/MKI67 Antibody Picoband®
View all Ki67/MKI67 Antibodies
SKU/Catalog Number
PB9026
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Ki67/MKI67 Antibody Picoband® catalog # PB9026. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ki67/MKI67 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9026)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Ki67 recombinant protein (Position: K2860-I3256).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9026 is reactive to MKI67 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
358 kDa
Calculated molecular weight
358694 MW
Background of Ki67/MKI67
Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9026 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunofluorescence, 5μg/ml, Human
Positive Control
WB: HELA Whole Cell, MCF-7 Whole Cell, COLO320 Whole Cell, HEPG2 Whole Cell, SKOV Whole Cell
IHC: human tonsil tissue
ICC: Hela cell
IF: human colon organoid tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Ki67 using anti-Ki67 antibody (PB9026).
Ki67 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ki67 Antibody (PB9026) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 2. Western blot analysis of Ki67 using anti-Ki67 antibody (PB9026).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA Whole Cell Lysate,
Lane 2: MCF-7 Whole Cell Lysate,
Lane 3: COLO320 Whole Cell Lysate,
Lane 4: HEPG2 Whole Cell Lysate,
Lane 5: SKOV Whole Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ki67 antigen affinity purified polyclonal antibody (Catalog # PB9026) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ki67 at approximately 358KD. The expected band size for Ki67 is at 358KD.
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Figure 3. IF analysis of Ki67 using anti-Ki67 antibody (PB9026).
Ki67 was detected in paraffin-embedded section of human colon organoid tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Ki67 Antibody (PB9026) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 4. ICC analysis of Ki67 using anti-Ki67 antibody (PB9026).
Ki67 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/ml rabbit anti-Ki67 Antibody (PB9026) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For MKI67 (Source: Uniprot.org, NCBI)
Gene Name
MKI67
Full Name
Proliferation marker protein Ki-67
Weight
358694 MW
Alternative Names
Antigen KI-67;MKI67; MKI67 KIA, MIB-, MIB-1, PPP1R105 marker of proliferation Ki-67 proliferation marker protein Ki-67| Ki67| by Ki-67|proliferation-related Ki-67 |protein phosphatase 1, regulatory subunit 105
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MKI67, check out the MKI67 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MKI67: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ki67/MKI67 Antibody Picoband® (PB9026)
Hello CJ!
PB9026 has been cited in 62 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Clinical biocharacterization of immunophenotype in hepatocellular carcinoma patients
Antimicrobial peptide 17BIPHE2 inhibits the proliferation of lung cancer cells in vitro and in vivo by regulating the ERK signaling pathway
PlexinA1 expression in gastric carcinoma and its relationship with tumor angiogenesis and proliferation
Elevated CREPT Expression Enhances the Progression of Salivary Gland Adenoid Cystic Carcinoma
Regulator of cullins-1 (ROC1) negatively regulates the Gli2 regulator SUFU to activate the hedgehog pathway in bladder cancer
R2: Identification of renal potential progenitor/stem cells that participate in the renal regeneration processes of kidney allograft fibrosis
Cryptotanshinone enhances the efficacy of Bcr-Abl tyrosine kinase inhibitors via inhibiting STAT3 and eIF4E signalling pathways in chronic myeloid leukaemia
LRRC4 mediates the formation of circular RNA CD44 to inhibit GBM cell proliferation
Effects of co-culturing BMSCs and auricular chondrocytes on the elastic modulus and hypertrophy of tissue engineered cartilage
Species: Mouse
LncRNA PVT1 promotes gemcitabine resistance of pancreatic cancer via activating Wnt/β-catenin and autophagy pathway through modulating the miR-619-5p/Pygo2 and miR-619-5p/ATG14 axes
Species: Human
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Customer Reviews
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1 Reviews For Anti-Ki67/MKI67 Antibody Picoband®
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Immunocytochemistry forAnti-P53/TP53 Antibody
Excellent
| Application | Immunohistochemistry (Paraffin-embedded) |
|---|---|
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Human tonsil |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 37°C for 30 minutes |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 2ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | 4°C overnight |
Verified Customer
Verified customer
Submitted 2019-07-19
Customer Q&As
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3 Customer Q&As for Anti-Ki67/MKI67 Antibody Picoband®
Question
We are currently using anti-Ki67/MKI67 antibody PB9026 for human tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
The anti-Ki67/MKI67 antibody (PB9026) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-17
Question
Is PB9026 suitable to stain ki67 with mouse and rat sample IHC-P sections?
Verified customer
Asked: 2019-05-15
Answer
The Anti-Ki67/MKI67 Antibody Picoband™ (PB9026) is only suitable for human samples.
Boster Scientific Support
Answered: 2019-05-16
Question
Has PB9026 been tested on mouse and rat samples for IHC-P?
Verified Customer
Verified customer
Asked: 2017-08-25
Answer
PB9026 is only suitable for human samples.
Boster Scientific Support
Answered: 2017-08-25
