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Product Info Summary
| SKU: | EK1348 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and cell lysates. |
Product info
Product Name
Human Livin ELISA Kit PicoKine®
SKU/Catalog Number
EK1348
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Livin ELISA Kit PicoKine™ (96 Tests). Quantitate Human BIRC7 in cell culture supernatants, serum and cell lysates. Sensitivity: 20pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Livin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1348)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: M1-S298
Sensitivity
<20 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1348 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1348 is reactive to BIRC7 in Human samples
Validated Sample Types
cell culture supernatants, serum and cell lysates.
Application Guarantee
EK1348 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Livin
BIRC7, also called KIAP or Livin, is a protein that in humans is encoded by the BIRC7 gene. This gene is mapped to 20q13.33. It encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. BIRC7 blocked apoptosis induced by BAX induction of cytochrome C release from mitochondria, and it also can block apoptosis induced by chemotherapeutic agents.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1348-CAP | Anti-Human BIRC7 Pre-coated 96-well strip microplate | 1 |
| EK1348-ST | Human BIRC7 Standard | 2 vials, 100 ng/tube |
| EK1348-DA | Human BIRC7 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.179 | 0.230 | 0.259 | 0.323 | 0.474 | 0.817 | 1.264 | 1.825 |
Data Example Images
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Human Livin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Livin, Dilution ratio of 1:1, concentration in serum is around 1.5 ng/ml..
Some articles we found to cite concentrations of Livin in samples: 20815986 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Livin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 344 | 1869 | 6693 | 362 | 1964 | 6956 |
| Standard deviation | 22.02 | 80.37 | 401.58 | 24.25 | 104.09 | 507.79 |
| CV (%) | 6.4% | 4.3% | 6% | 6.7% | 5.3% | 7.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Livin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 344 | 365 | 383 | 379 | 368 | 15.08 | 4.1% |
| Sample 2 | 1869 | 1916 | 1940 | 1875 | 1900 | 98.8 | 5.2% |
| Sample 3 | 6693 | 6845 | 7026 | 6968 | 6883 | 371.68 | 5.4% |
Protein Target Info & Infographic
Gene/Protein Information For BIRC7 (Source: Uniprot.Org, NCBI)
Gene Name
BIRC7
Full Name
Baculoviral IAP repeat-containing protein 7
Weight
Superfamily
IAP family
Alternative Names
baculoviral IAP repeat containing 7; baculoviral IAP repeat-containing 7; BIRC7; KIAP; KIAPRING finger protein 50; Kidney inhibitor of apoptosis protein; livin inhibitor-of-apoptosis; Livin; Melanoma inhibitor of apoptosis protein; ML-IAP; MLIAPlivin inhibitor of apoptosis; ML-IAPmliap; RNF50LIVINbaculoviral IAP repeat-containing protein 7 BIRC7 KIAP, LIVIN, ML-IAP, MLIAP, RNF50 baculoviral IAP repeat containing 7 baculoviral IAP repeat-containing protein 7|RING finger protein 50|RING-type E3 ubiquitin transferase BIRC7|kidney inhibitor of apoptosis protein|livin inhibitor of apoptosis|melanoma inhibitor of apoptosis protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on BIRC7, check out the BIRC7 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for BIRC7: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Livin ELISA Kit PicoKine® (EK1348)
Hello CJ!
EK1348 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
LncRNA CCAT1 inhibits cell apoptosis of renal cell carcinoma through up-regulation of Livin protein
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Human Livin ELISA Kit PicoKine®
Question
Q: how to proceed with the analysis of ELISA data? I measured Livin level in serum.
T. Phillips
Verified customer
Asked: 2019-10-01
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-10-01
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Livin using the EK1348 Human Livin Picokine® ELISA Kit?
E. Johnson
Verified customer
Asked: 2019-02-17
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2019-02-17
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
D. Campbell
Verified customer
Asked: 2018-11-02
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-11-02
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
C. Li
Verified customer
Asked: 2018-10-20
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-10-20
Question
Q: What is the optimal O.D. value for Livin ELISA kit? I used your Livin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Livin even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-08-15
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Livin ELISA kit to have sensitivity of 20pg/ml, that means the minimum amount of Livin that can be declared/interpreted as positive by the above standard is 20pg/ml.
Boster Scientific Support
Answered: 2018-08-15
Question
Q: Can Livin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-06-04
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-06-04
Question
Q: if the enzyme conjugated Livin antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Livin antigen?
J. Zhang
Verified customer
Asked: 2018-01-21
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-01-21
