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Product Info Summary
| SKU: | PA1918 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Ki67/MKI67 Antibody Picoband®
View all Ki67/MKI67 Antibodies
SKU/Catalog Number
PA1918
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Ki67/MKI67 Antibody catalog # PA1918. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ki67/MKI67 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1918)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Ki67.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1918 is reactive to MKI67 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22-24 kDa
Calculated molecular weight
358694 MW
Background of Ki67/MKI67
Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, Schonk et al. (1989) demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1918 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Anti-Ki67 antibody, PA1918, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human Ki67 50.3KD
(162aa tag+ K2967-I3256)
Lane 1: Recombinant Human Ki67 Protein 10ng
Lane 2: Recombinant Human Ki67 Protein 5ng
Lane 3: Recombinant Human Ki67 Protein 2.5ng
Protein Target Info & Infographic
Gene/Protein Information For MKI67 (Source: Uniprot.org, NCBI)
Gene Name
MKI67
Full Name
Proliferation marker protein Ki-67
Weight
358694 MW
Alternative Names
Antigen KI-67;MKI67; MKI67 KIA, MIB-, MIB-1, PPP1R105 marker of proliferation Ki-67 proliferation marker protein Ki-67| Ki67| by Ki-67|proliferation-related Ki-67 |protein phosphatase 1, regulatory subunit 105
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MKI67, check out the MKI67 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MKI67: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ki67/MKI67 Antibody Picoband® (PA1918)
Hello CJ!
PA1918 has been cited in 48 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yang Z,Zhang J,Lu D,Sun Y, Zhao X,Wang X,Zhou W,He Q,Jiang Z.Hsa_circ_0137008 suppresses the malignant phenotype in colorectal cancer by acting as a microRNA-338-5p sponge. Cancer Cell Int.2020 Mar 4;20:67. doi:10.1186/s1293 5-020-1150-1.PMID:32158357;PMC
Species: Human
PA1918 usage in article: APP:WB, SAMPLE:SW480 AND HCT116 CELLS, DILUTION:NA
KDM4B and KDM4A promote endometrial cancer progression by regulating androgen receptor, c-myc, and p27kip1
The induction of autophagy against mitochondria-mediated apoptosis in lung cancer cells by a ruthenium (II) imidazole complex
715: GNRs@SiO2-FA in combination with radiotherapy induces the apoptosis of HepG2 cells by modulating the expression of apoptosis-related proteins
Mutant p53 induces EZH2 expression and promotes epithelial?mesenchymal transition by disrupting p68-Drosha complex assembly and attenuating miR-26a processing
Solamargine triggers hepatoma cell death through apoptosis
WX-132-18B, a novel microtubule inhibitor, exhibits promising anti-tumor effects
Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium
HER2-siRNA delivered by EGFR-specific single chain antibody inhibits NSCLC cell proliferation and tumor growth
Tumor-suppressive function of long noncoding RNA MALAT1 in glioma cells by downregulation of MMP2 and inactivation of ERK/MAPK signaling
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