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Product Info Summary
| SKU: | FC01572-Biotin |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-human CD22/Siglec 2 Monoclonal Antibody Biotin Conjugated, Flow Validated
View all Siglec-2/CD22 Antibodies
SKU/Catalog Number
FC01572-Biotin
Size
100ug
Form
Liquid
Description
Boster Bio Anti-human CD22/Siglec 2 Monoclonal Antibody Biotin Conjugated, Flow Validated (Catalog# FC01572-Biotin). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD22/Siglec 2 Monoclonal Antibody Biotin Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC01572-Biotin)
Host
Mouse
Contents
PBS pH 7.2, 0.09% sodium azide
Clonality
Monoclonal
Clone Number
Clone: MYG13
Isotype
IgG1,k
Immunogen
Human Raji Cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC01572-Biotin is reactive to CD22 in Human
Reconstitution
Calculated molecular weight
95348 MW
Background of Siglec-2/CD22
CD22 or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. This gene is mapped to 19q13.2. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. Generally speaking, CD22 is a regulatory molecule that prevents the overactivation of the immune system and the development of autoimmune diseases. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signalling. This gene can downmodulate signaling through the IgM and IgD B-cell receptors (BCRs), but not through the IgG BCR, because the IgG cytoplasmic tail prevents CD22 phosphorylation and actually enhances IgG-BCR signaling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC01572-Biotin is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Lymphocytes gated PBMCs stained with biotin conjugated anti-human CD22 (clone MYG13) followed by staining with SA-PE (red histogram). PBMCs lymphocytes stained with biotin conjugated IgG1 isotype control followed by staining with SA-PE (blue histogram).
Protein Target Info & Infographic
Gene/Protein Information For CD22 (Source: Uniprot.org, NCBI)
Gene Name
CD22
Full Name
B-cell receptor CD22
Weight
95348 MW
Superfamily
immunoglobulin superfamily
Alternative Names
B-cell receptor CD22;B-lymphocyte cell adhesion molecule;BL-CAM;Sialic acid-binding Ig-like lectin 2;Siglec-2;T-cell surface antigen Leu-14;CD22;CD22;SIGLEC2; CD22 SIGLEC-2, SIGLEC2 CD22 molecule B-cell receptor CD22|B-lymphocyte cell adhesion molecule|BL-CAM|CD22 |T-cell surface Leu-14|sialic acid-binding Ig-like lectin 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD22, check out the CD22 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD22/Siglec 2 Monoclonal Antibody Biotin Conjugated, Flow Validated (FC01572-Biotin)
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No publications found for FC01572-Biotin
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Customer Q&As
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10 Customer Q&As for Anti-human CD22/Siglec 2 Monoclonal Antibody Biotin Conjugated, Flow Validated
Question
Here is the WB image, lot number and protocol we used for spleen using anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated FC01572-Biotin. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-30
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for spleen using anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated FC01572-Biotin. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-03
Question
Does FC01572-Biotin anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
It shows on the product datasheet, FC01572-Biotin anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-21
Question
Is a blocking peptide available for product anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated (FC01572-Biotin)?
Verified Customer
Verified customer
Asked: 2018-11-16
Answer
We do provide the blocking peptide for product anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated (FC01572-Biotin). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-11-16
Question
I see that the anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated FC01572-Biotin works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
R. Carter
Verified customer
Asked: 2018-01-04
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-01-04
Question
Does anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated FC01572-Biotin work for Flow Cytometry with spleen?
D. Thomas
Verified customer
Asked: 2017-11-20
Answer
According to the expression profile of spleen, CD22 is highly expressed in spleen. So, it is likely that anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated FC01572-Biotin will work for Flow Cytometry with spleen.
Boster Scientific Support
Answered: 2017-11-20
Question
I was wanting to use your anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated for Flow Cytometry for human spleen on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human spleen identification?
Verified Customer
Verified customer
Asked: 2017-07-11
Answer
It shows on the product datasheet, FC01572-Biotin anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human spleen in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-11
Question
Is there a BSA free version of anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated FC01572-Biotin available?
N. Jackson
Verified customer
Asked: 2017-06-06
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated FC01572-Biotin are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-06-06
Question
Can you help my question with product FC01572-Biotin, anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-05-31
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC01572-Biotin anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-31
Question
My question regards to test anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated FC01572-Biotin on human spleen for research purposes, then I may be interested in using anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated FC01572-Biotin for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Wu
Verified customer
Asked: 2013-07-16
Answer
The products we sell, including anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated FC01572-Biotin, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-07-16
