Click image to see more details
Product Info Summary
| SKU: | EK1977 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse FCRL1 ELISA Kit PicoKine®
View all FCRLA/FCRLM1 ELISA kits
SKU/Catalog Number
EK1977
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse FCRL1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Fcrla in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse FCRL1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1977)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A17-S219
Sensitivity
<50 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1977 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1977 is reactive to Fcrla in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1977 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FCRLA/FCRLM1
Fc receptor-like protein 1 is a protein that in humans is encoded by the FCRL1 gene. It is mapped to 3; 3 F1.This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein contains three extracellular C2-like immunoglobulin domains, a transmembrane domain and a cytoplasmic domain with two immunoreceptor-tyrosine activation motifs. This protein may play a role in the regulation of cancer cell growth. Alternative splicing results in multiple transcript variants.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1977-CAP | Anti-Mouse Fcrla Pre-coated 96-well strip microplate | 1 |
| EK1977-ST | Mouse Fcrla Standard | 2 vials, 10 ng/tube |
| EK1977-DA | Mouse Fcrla Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.041 | 0.096 | 0.166 | 0.296 | 0.583 | 1.027 | 1.674 | 2.305 |
Data Example Images
Click image to see more details
Mouse FCRL1 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FCRLA/FCRLM1, Dilution ratio of 1:1, concentration in serum and plasma is around 200 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse FCRL1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 90 | 464 | 1704 | 96 | 476 | 1814 |
| Standard deviation | 4.41 | 21.81 | 69.86 | 5.38 | 24.28 | 83.44 |
| CV (%) | 4.9% | 4.7% | 4.1% | 5.6% | 5.1% | 4.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of FCRLA/FCRLM1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 90 | 96 | 85 | 88 | 89 | 4.02 | 4.5% |
| Sample 2 | 464 | 409 | 476 | 420 | 442 | 28.34 | 6.4% |
| Sample 3 | 1704 | 1617 | 1595 | 1596 | 1628 | 44.74 | 2.7% |
Protein Target Info & Infographic
Gene/Protein Information For Fcrla (Source: Uniprot.Org, NCBI)
Gene Name
Fcrla
Full Name
Fc receptor-like A
Weight
38.927kDa
Alternative Names
Fc receptor-like A; Fc receptor homolog expressed in B-cells; Fc receptor-like and mucin-like protein 1; Fc receptor-like protein; Fc receptor-related protein X; FcRX; Fcrla; Fcrl; Fcrl1; Fcrlm1; Fcrx; Freb FCRLA FCRL, FCRL1, FCRLM1, FCRLX, FCRLb, FCRLc1, FCRLc2, FCRLd, FCRLe, FCRX, FREB Fc receptor like A Fc receptor-like A|Fc receptor homolog expressed in B cells (FREB)|Fc receptor related protein X|Fc receptor-like and mucin-like 1|fc receptor homolog expressed in B-cells|fc receptor-like and mucin-like protein 1|fc receptor-like protein|fc receptor-related protein X
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Fcrla, check out the Fcrla Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Fcrla: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse FCRL1 ELISA Kit PicoKine® (EK1977)
Hello CJ!
No publications found for EK1977
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Mouse FCRL1 ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Mouse FCRL1 ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Mouse FCRL1 ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
K. Tiwari
Verified customer
Asked: 2020-12-21
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-12-21
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement FCRLA using the EK1977 Mouse FCRLA Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-10-08
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2020-10-08
Question
Q: What is the optimal O.D. value for FCRLA ELISA kit? I used your FCRLA ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain FCRLA even though the O.D. values are not very high?
A. Hill
Verified customer
Asked: 2020-04-28
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this FCRLA ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of FCRLA that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2020-04-28
Question
Q: if the enzyme conjugated FCRLA antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the FCRLA antigen?
K. Kaur
Verified customer
Asked: 2019-01-08
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-01-08
Question
Q: how to analyze ELISA data? I have obtained FCRLA level in plasma.
Verified Customer
Verified customer
Asked: 2017-08-03
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-08-03
Question
Q: how can I thaw whole blood sample for FCRLA ELISA after freezing?
Verified Customer
Verified customer
Asked: 2016-04-01
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test FCRLA for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2016-04-01
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
G. Lopez
Verified customer
Asked: 2014-06-05
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2014-06-05
