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Product Info Summary
| SKU: | RP1027 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Angiostatin K1-3/PLG Antibody Picoband®
SKU/Catalog Number
RP1027
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Angiostatin K1-3/PLG Antibody catalog # RP1027. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Angiostatin K1-3/PLG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1027)
Host
Rabbit
Contents
Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Angiostatin K1-3 recombinant protein (Position: C103-C352).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1027 is reactive to PLG in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
90-100 kDa
Calculated molecular weight
90569 MW
Background of PLG
Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity (ED50 = 70nM) relative to kringles 1-4 (ED50 = 135nM).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1027 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human placenta tissue, human CACO-2 whole cell
IHC: human mammary cancer tissue, human placenta tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Angiostatin K1-3 using anti-Angiostatin K1-3 antibody (RP1027).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human CACO-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiostatin K1-3 antigen affinity purified polyclonal antibody (Catalog # RP1027) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiostatin K1-3 at approximately 90-100 kDa. The expected band size for Angiostatin K1-3 is at 91 kDa.
Click image to see more details
Figure 2. IHC analysis of Angiostatin K1-3 using anti-Angiostatin K1-3 antibody (RP1027).
Angiostatin K1-3 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Angiostatin K1-3 Antibody (RP1027) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Angiostatin K1-3 using anti-Angiostatin K1-3 antibody (RP1027).
Angiostatin K1-3 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Angiostatin K1-3 Antibody (RP1027) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PLG (Source: Uniprot.org, NCBI)
Gene Name
PLG
Full Name
Plasminogen
Weight
90569 MW
Superfamily
peptidase S1 family
Alternative Names
Plasminogen;3.4.21.7;Plasmin heavy chain A;Activation peptide;Angiostatin;Plasmin heavy chain A, short form;Plasmin light chain B;PLG; PLG plasminogen plasminogen|plasmin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLG, check out the PLG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Angiostatin K1-3/PLG Antibody Picoband® (RP1027)
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No publications found for RP1027
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-Angiostatin K1-3/PLG Antibody Picoband®
Question
We are currently using anti-Angiostatin K1-3/PLG antibody RP1027 for human tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
S. Carter
Verified customer
Asked: 2019-12-09
Answer
The anti-Angiostatin K1-3/PLG antibody (RP1027) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-09
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right lobe of liver using anti-Angiostatin K1-3/PLG antibody RP1027. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-19
Question
We are interested in using your anti-Angiostatin K1-3/PLG antibody for activation of matrix metalloproteinases studies. Has this antibody been tested with western blotting on hepg2 whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
Thank you for your inquiry. This RP1027 anti-Angiostatin K1-3/PLG antibody is validated on hepg2 whole cell lysates. It is guaranteed to work for ELISA, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-18
Question
Does anti-Angiostatin K1-3/PLG antibody RP1027 work on pig ELISA with liver?
S. Parker
Verified customer
Asked: 2019-08-29
Answer
Our lab technicians have not validated anti-Angiostatin K1-3/PLG antibody RP1027 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Angiostatin K1-3/PLG antibody RP1027 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig liver in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-29
Question
I was wanting to use your anti-Angiostatin K1-3/PLG antibody for WB for human right lobe of liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human right lobe of liver identification?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
As indicated on the product datasheet, RP1027 anti-Angiostatin K1-3/PLG antibody has been validated for ELISA, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human right lobe of liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-09
Question
Will anti-Angiostatin K1-3/PLG antibody RP1027 work for WB with right lobe of liver?
W. Singh
Verified customer
Asked: 2019-07-04
Answer
According to the expression profile of right lobe of liver, PLG is highly expressed in right lobe of liver. So, it is likely that anti-Angiostatin K1-3/PLG antibody RP1027 will work for WB with right lobe of liver.
Boster Scientific Support
Answered: 2019-07-04
Question
I see that the anti-Angiostatin K1-3/PLG antibody RP1027 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-04-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-02
Question
We bought anti-Angiostatin K1-3/PLG antibody for ELISA on right lobe of liver in a previous project. I am using human, and We want to use the antibody for IHC next. I would like examining right lobe of liver as well as milk in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2018-09-18
Answer
I have checked the website and datasheets of our anti-Angiostatin K1-3/PLG antibody and it seems that RP1027 has been validated on human in both ELISA and IHC. Thus RP1027 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-09-18
Question
Is there a BSA free version of anti-Angiostatin K1-3/PLG antibody RP1027 available?
Verified Customer
Verified customer
Asked: 2017-12-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Angiostatin K1-3/PLG antibody RP1027 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-26
Question
Is this RP1027 anti-Angiostatin K1-3/PLG antibody reactive to the isotypes of PLG?
Verified Customer
Verified customer
Asked: 2017-12-11
Answer
The immunogen of RP1027 anti-Angiostatin K1-3/PLG antibody is E. coli-derived human Angiostatin K1-3 recombinant protein(Position: C103-C352). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-11
Question
We were content with the WB result of your anti-Angiostatin K1-3/PLG antibody. However we have seen positive staining in kidney secreted using this antibody. Is that expected? Could you tell me where is PLG supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-07-06
Answer
Based on literature, kidney does express PLG. Generally PLG expresses in secreted. Regarding which tissues have PLG expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 14574404, 24275569
Milk, Pubmed ID: 18780401
Boster Scientific Support
Answered: 2017-07-06
Question
you antibody to test anti-Angiostatin K1-3/PLG antibody RP1027 on human right lobe of liver for research purposes, then I may be interested in using anti-Angiostatin K1-3/PLG antibody RP1027 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Jones
Verified customer
Asked: 2017-01-09
Answer
The products we sell, including anti-Angiostatin K1-3/PLG antibody RP1027, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-01-09
Question
Is a blocking peptide available for product anti-Angiostatin K1-3/PLG antibody (RP1027)?
C. Mitchell
Verified customer
Asked: 2015-05-14
Answer
We do provide the blocking peptide for product anti-Angiostatin K1-3/PLG antibody (RP1027). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-05-14
Question
We have observed staining in human liver. What should we do? Is anti-Angiostatin K1-3/PLG antibody supposed to stain liver positively?
M. Williams
Verified customer
Asked: 2015-04-06
Answer
From literature liver does express PLG. From Uniprot.org, PLG is expressed in right lobe of liver, liver, kidney, milk, among other tissues. Regarding which tissues have PLG expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 14574404, 24275569
Milk, Pubmed ID: 18780401
Boster Scientific Support
Answered: 2015-04-06
Question
See below the WB image, lot number and protocol we used for right lobe of liver using anti-Angiostatin K1-3/PLG antibody RP1027. Please let me know if you require anything else.
P. Zhang
Verified customer
Asked: 2014-10-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-15
Question
I have a question about product RP1027, anti-Angiostatin K1-3/PLG antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Yang
Verified customer
Asked: 2014-09-02
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1027 anti-Angiostatin K1-3/PLG antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-09-02
Question
Does RP1027 anti-Angiostatin K1-3/PLG antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Rodriguez
Verified customer
Asked: 2013-11-21
Answer
As indicated on the product datasheet, RP1027 anti-Angiostatin K1-3/PLG antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-11-21
