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Product Info Summary
| SKU: | A03674-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Plasminogen/PLG Antibody Picoband®
SKU/Catalog Number
A03674-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Plasminogen/PLG Antibody Picoband® catalog # A03674-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Plasminogen/PLG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03674-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Plasminogen, which shares 72.7% amino acid (aa) sequence identity with both mouse and rat Plasminogen.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03674-1 is reactive to PLG in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated molecular weight
28461 MW
Background of PLG
Plasminogen (PLG) is a circulating zymogen that is converted to the active enzyme plasmin by cleavage of the peptide bond between arg560 and val561, which is mediated by urokinase (PLAU) and tissue plasminogen activator (PLAT). The main function of plasmin is to dissolve fibrin (see, e.g., FGA) clots. Plasmin, like trypsin, belongs to the family of serine proteinases. Defects in this gene are likely a cause of thrombophilia and ligneous conjunctivitis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03674-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human HL-60 whole cell, human HEK293 whole cell, human Caco-2 whole cell, human Sw620 whole cell, human Jurkat whole cell, human K562 whole cell, rat liver tissue, mouse brain tissue
FCM: U251 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Plasminogen using anti-Plasminogen antibody (A03674-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human Sw620 whole cell lysates,
Lane 5: human Jurkat whole cell lysates,
Lane 6: human K562 whole cell lysates,
Lane 7: rat liver tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plasminogen antigen affinity purified polyclonal antibody (Catalog # A03674-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plasminogen at approximately 100 kDa. The expected band size for Plasminogen is at 90 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of U251 cells using anti-Plasminogen antibody (A03674-1).
Overlay histogram showing U251 cells stained with A03674-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Plasminogen Antibody (A03674-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For PLG (Source: Uniprot.org, NCBI)
Gene Name
PLG
Full Name
Plasminogen
Weight
28461 MW
Superfamily
peptidase S1 family
Alternative Names
Plasminogen; Plasmin heavy chain A; Activation peptide; Angiostatin; Plasmin heavy chain A, short form; Plasmin light chain B; PLG PLG plasminogen plasminogen|plasmin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLG, check out the PLG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Plasminogen/PLG Antibody Picoband® (A03674-1)
Hello CJ!
A03674-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Increased plasmin-mediated proteolysis of L1CAM in a mouse model of idiopathic normal pressure hydrocephalus
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Plasminogen/PLG Antibody Picoband®
Question
We want using your anti-Plasminogen/PLG antibody for degradation of the extracellular matrix studies. Has this antibody been tested with western blotting on 22rv1 whole cell lysate? We would like to see some validation images before ordering.
A. Mangal
Verified customer
Asked: 2020-04-23
Answer
Thank you for your inquiry. This A03674-1 anti-Plasminogen/PLG antibody is tested on placenta tissue, human placenta tissue, 22rv1 whole cell lysate, rat brain tissue, mouse brain. It is guaranteed to work for Flow Cytometry, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-23
Question
Is there a BSA free version of anti-Plasminogen/PLG antibody A03674-1 available?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Plasminogen/PLG antibody A03674-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-16
Question
I see that the anti-Plasminogen/PLG antibody A03674-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-26
Question
Our lab want to know about to test anti-Plasminogen/PLG antibody A03674-1 on mouse liver for research purposes, then I may be interested in using anti-Plasminogen/PLG antibody A03674-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
The products we sell, including anti-Plasminogen/PLG antibody A03674-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-06
Question
My colleagues were happy with the WB result of your anti-Plasminogen/PLG antibody. However we have seen positive staining in liver secreted using this antibody. Is that expected? Could you tell me where is PLG supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
From literature, liver does express PLG. Generally PLG expresses in secreted. Regarding which tissues have PLG expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 14574404, 24275569
Milk, Pubmed ID: 18780401
Boster Scientific Support
Answered: 2019-10-17
Question
Can you help my question with product A03674-1, anti-Plasminogen/PLG antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03674-1 anti-Plasminogen/PLG antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Plasminogen/PLG antibody A03674-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-18
Question
Would anti-Plasminogen/PLG antibody A03674-1 work for WB with liver?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
According to the expression profile of liver, PLG is highly expressed in liver. So, it is likely that anti-Plasminogen/PLG antibody A03674-1 will work for WB with liver.
Boster Scientific Support
Answered: 2019-09-04
Question
We have been able to see staining in rat milk. What should we do? Is anti-Plasminogen/PLG antibody supposed to stain milk positively?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
From what I have seen in literature milk does express PLG. From what I have seen in Uniprot.org, PLG is expressed in right lobe of liver, liver, kidney, milk, among other tissues. Regarding which tissues have PLG expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 14574404, 24275569
Milk, Pubmed ID: 18780401
Boster Scientific Support
Answered: 2019-07-31
Question
We have tried in the past anti-Plasminogen/PLG antibody for Flow Cytometry on milk in the past. I am using human, and We intend to use the antibody for WB next. We need examining milk as well as right lobe of liver in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-04-03
Answer
I looked at the website and datasheets of our anti-Plasminogen/PLG antibody and I see that A03674-1 has been validated on human in both Flow Cytometry and WB. Thus A03674-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-04-03
Question
Is a blocking peptide available for product anti-Plasminogen/PLG antibody (A03674-1)?
Verified Customer
Verified customer
Asked: 2018-11-20
Answer
We do provide the blocking peptide for product anti-Plasminogen/PLG antibody (A03674-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-11-20
Question
See attached the WB image, lot number and protocol we used for liver using anti-Plasminogen/PLG antibody A03674-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-09-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-13
Question
I was wanting to use your anti-Plasminogen/PLG antibody for WB for mouse liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2017-07-27
Answer
You can see on the product datasheet, A03674-1 anti-Plasminogen/PLG antibody has been tested for Flow Cytometry, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-27
Question
Does A03674-1 anti-Plasminogen/PLG antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-05-17
Answer
You can see on the product datasheet, A03674-1 anti-Plasminogen/PLG antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-05-17
Question
We are currently using anti-Plasminogen/PLG antibody A03674-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
T. Li
Verified customer
Asked: 2017-01-12
Answer
The anti-Plasminogen/PLG antibody (A03674-1) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-01-12
Question
Is this A03674-1 anti-Plasminogen/PLG antibody reactive to the isotypes of PLG?
T. Huang
Verified customer
Asked: 2015-03-12
Answer
The immunogen of A03674-1 anti-Plasminogen/PLG antibody is A synthetic peptide corresponding to a sequence of human Plasminogen (EPLDDYVNTQGASLFSVTKKQL). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-03-12
