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Product Info Summary
| SKU: | M01777 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Primate |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC-F |
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Product info
Product Name
Anti-Hu CD83 Purified Monoclonal Antibody
SKU/Catalog Number
M01777
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD83 Purified Monoclonal Antibody (Catalog# M01777). Tested in Flow Cytometry, IHC-F application(s). This antibody reacts with Human, Primate.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD83 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01777)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
HB15e
Isotype
Mouse IgG1 kappa
Immunogen
Human CD83-transfected Cos cells. The mouse monoclonal antibody HB15e recognizes an extracellular epitope of CD83, a 40-45 kDa type I glycoprotein expressed on mature dendritic cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01777 is reactive to CD83 in Human, Primate
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
23042 MW
Background of CD83
CD83 is a 40-45 kDa heavily glycosylated type I cell surface glycoprotein of immunoglobulin family. It is expressed on the surface of mature dendritic cells, Langerhans cells in the skin, and interdigitating reticulum cells in the lymphoid tissues. Low expression of CD83 has been reported in activated T and B cells. Cytoplasmic expression of CD83 can be detected also in monocytes and macrophages. CD83 is involved in modulation of antigen presentation. Soluble CD83 has immunoregulatory functions, it is able to down-regulate dendritic cell maturation and stimulation of T cells. In the developing immune system, release of soluble CD83 from dendritic cells upon stimulation by gram-positive or gram-negative bacteria has anti-allergic effect. Herpes simplex virus, on the other hand, causes CD83 degradation in mature dendritic cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01777 is guaranteed for Flow Cytometry, IHC-F Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 µg/mL, Positive control: peripheral blood-derived dendritic cells.
Immunohistochemistry (frozen sections): acetone fixation.
Validation Images & Assay Conditions
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Flow cytometry surface staining pattern of human interferon alpha stimulated peripheral whole blood stained using anti-human CD83 (HB15e) purified antibody (concentration in sample 2 μg/ml, GAM APC).
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Separation of human monocytes (red-filled) from CD83 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human interferon alpha stimulated peripheral whole blood stained using anti-human CD83 (HB15e) purified antibody (concentration in sample 2 μg/ml, GAM APC).
Protein Target Info & Infographic
Gene/Protein Information For CD83 (Source: Uniprot.org, NCBI)
Gene Name
CD83
Full Name
CD83 antigen
Weight
23042 MW
Alternative Names
BL11, HB15 CD83 BL11, HB15 CD83 molecule CD83 |B-cell activation protein|CD83 (activated B lymphocytes, immunoglobulin superfamily)|cell surface protein HB15|cell-surface glycoprotein|hCD83
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD83, check out the CD83 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD83: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD83 Purified Monoclonal Antibody (M01777)
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