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Product Info Summary
| SKU: | FEK0715 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin) |
| Assay Time: | 90 Minutes |
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How Quick Picokine(R) ELISA kits work: Get data in 90 minutes
Product info
Product Name
Human CD163/M130 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0715
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD163 / M130 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CD163 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 150pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human CD163/M130 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0715)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: G41-S1045
Sensitivity
<150 pg/ml
Assay Range
1.56 ng/ml - 100 ng/ml
Standard Dilution Instructions
See datasheet of FEK0715 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0715 is reactive to CD163 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin)
Application Guarantee
FEK0715 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD163
CD163 (Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich (SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0715-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0715-ST | Human CD163 Standard | 2 | 100 ng/tube |
| FEK0715-A | Human CD163 Antibody A | 1 | 3ml |
| FEK0715-B | Human CD163 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.011 | 0.255 | 0.405 | 0.794 | 1.291 | 1.820 | 2.205 | 2.478 |
Data Example Images
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Human CD163 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD163/M130 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 3520 | 11456 | 44674 | 3368 | 11294 | 48653 |
| Standard deviation | 144.32 | 526.97 | 303195.343 | 195.34 | 632.46 | 3357.05 |
| CV (%) | 4.1% | 4.6% | 6.8% | 5.8% | 5.6% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD163 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 3520 | 3403 | 3222 | 3050 | 3298 | 178.59 | 5.4% |
| Sample 2 | 11456 | 11119 | 13238 | 12078 | 11972 | 807.44 | 6.7% |
| Sample 3 | 44674 | 42932 | 42302 | 44756 | 43666 | 1072.77 | 2.4% |
Protein Target Info & Infographic
Gene/Protein Information For CD163 (Source: Uniprot.Org, NCBI)
Gene Name
CD163
Full Name
Scavenger receptor cysteine-rich type 1 protein M130
Weight
125451 MW
Alternative Names
Scavenger receptor cysteine-rich type 1 protein M130;Hemoglobin scavenger receptor;CD163;Soluble CD163;sCD163;CD163;M130; CD163 M130, MM130, SCARI1 CD163 molecule scavenger receptor cysteine-rich type 1 protein M130|hemoglobin scavenger receptor|macrophage-associated
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD163, check out the CD163 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD163: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD163/M130 PicoKine® Quick ELISA Kit (FEK0715)
Hello CJ!
FEK0715 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
SIV Infection Impairs the Central Nervous System in Chinese Rhesus Macaques
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Customer Q&As
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1 Customer Q&As for Human CD163/M130 PicoKine® Quick ELISA Kit
Question
Hello Support: I would like to know if this Human CD163 / M130 PicoKine
Verified Customer
Verified customer
Asked: 2018-03-08
Answer
The product Human CD163 / M130 PicoKine
Boster Scientific Support
Answered: 2018-03-08
