Anti-Hu CD33 Purified Monoclonal Antibody

Product Name

Anti-Hu CD33 Purified Monoclonal Antibody

View all Siglec-3/CD33 Antibodies

SKU/Catalog Number

M01508-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD33 Purified Monoclonal Antibody (Catalog# M01508-1). Tested in Flow Cytometry, IP, WB, IHC-F, ICC, MC (CyTOF) application(s). This antibody reacts with Primate, Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD33 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01508-1)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

WM53

Isotype

Mouse IgG1

Immunogen

Human AML cells. The mouse monoclonal antibody WM53 reacts with an extracellular epitope of CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01508-1 is reactive to CD33 in Human, Primate

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

39825 MW

Background of Siglec-3/CD33

CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M01508-1 is guaranteed for Flow Cytometry, IP, IHC-F, ICC, WB, CyTOF Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (frozen sections): Acetone fixation.
Flow cytometry: 1-4 µg/ml

Validation Images & Assay Conditions

Gene/Protein Information For CD33 (Source: Uniprot.org, NCBI)

Alternative Names

SIGLEC3, p67 CD33 SIGLEC-3, SIGLEC3, p67 CD33 molecule myeloid cell surface CD33|CD33 (gp67)|CD33 molecule transcript|gp67|sialic acid-binding Ig-like lectin 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis