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Product Info Summary
| SKU: | FC01508 |
|---|---|
| Size: | 100ug, 500ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-human CD33/Siglec 3 Monoclonal Antibody Unconjugated, Flow Validated
View all Siglec-3/CD33 Antibodies
SKU/Catalog Number
FC01508
Size
100ug, 500ug
Form
Liquid
Description
Boster Bio Anti-human CD33/Siglec 3 Monoclonal Antibody Unconjugated, Flow Validated (Catalog# FC01508). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD33/Siglec 3 Monoclonal Antibody Unconjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC01508)
Host
Mouse
Contents
PBS pH 7.2, 0.1% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: WM53
Isotype
IgG1,k
Immunogen
Human AML cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC01508 is reactive to CD33 in Human
Reconstitution
Calculated molecular weight
39825 MW
Background of Siglec-3/CD33
CD33, also known as Siglec-3 (sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC-3, gp67, p67), is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells. CD33 binds sialic acids, therefore is a member of the SIGLEC family of lectins. By fluorescence in situ hybridization, CD33 is mapped to 19q13.3-q13.4.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC01508 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Monocytes gated human PBMCs stained with mouse anti-human CD33 (clone WM53) followed by staining with goat anti-mouse IgG(H+L) Alexa Fluor 488 (red histogram). Blue histogram is for the monocytes gated PBMCs stained with purified mouse IgG1 (MOPC31C) isotype control followed by staining with goat anti-mouse IgG(H+L) Alexa Fluor 488.
Protein Target Info & Infographic
Gene/Protein Information For CD33 (Source: Uniprot.org, NCBI)
Gene Name
CD33
Full Name
Myeloid cell surface antigen CD33
Weight
39825 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Myeloid cell surface antigen CD33;Sialic acid-binding Ig-like lectin 3;Siglec-3;gp67;CD33;CD33;SIGLEC3; CD33 SIGLEC-3, SIGLEC3, p67 CD33 molecule myeloid cell surface CD33|CD33 (gp67)|CD33 molecule transcript|gp67|sialic acid-binding Ig-like lectin 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD33, check out the CD33 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD33: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD33/Siglec 3 Monoclonal Antibody Unconjugated, Flow Validated (FC01508)
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No publications found for FC01508
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Customer Q&As
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2 Customer Q&As for Anti-human CD33/Siglec 3 Monoclonal Antibody Unconjugated, Flow Validated
Question
I am interested in to test anti-human CD33/Siglec 3 Monoclonal antibody Unconjugated, Flow Validated FC01508 on human leukocyte for research purposes, then I may be interested in using anti-human CD33/Siglec 3 Monoclonal antibody Unconjugated, Flow Validated FC01508 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
The products we sell, including anti-human CD33/Siglec 3 Monoclonal antibody Unconjugated, Flow Validated FC01508, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-27
Question
I was wanting to use your anti-human CD33/Siglec 3 Monoclonal antibody Unconjugated, Flow Validated for Flow Cytometry for human leukocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukocyte identification?
E. Patel
Verified customer
Asked: 2018-07-25
Answer
You can see on the product datasheet, FC01508 anti-human CD33/Siglec 3 Monoclonal antibody Unconjugated, Flow Validated has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-25
