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Key=entity_id, value=9059
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Key=sku, value=FC01508-Biotin
Key=has_options, value=0
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Key=created_at, value=2016-11-10 02:27:32
Key=updated_at, value=2024-09-26 00:48:21
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=Anti-human CD33/Siglec 3 Monoclonal Antibody Biotin Conjugated, Flow Validated
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Key=is_recurring, value=No
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Key=conjugate, value=Biotin
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Key=template, value=antibodies
Key=host, value=Mouse
Key=quantity_and_stock_status
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Key=price, value=215.0000
Key=weight, value=1.0000
Key=meta_title, value=Anti-human CD33/Siglec 3 Monoclonal Antibody Biotin Conjugated, Flow Validated
Key=meta_description, value=Boster Bio Anti-human CD33/Siglec 3 Monoclonal Antibody Biotin Conjugated, Flow Validated (Catalog# FC01508-Biotin). Tested in Flow Cytometry application(s). This antibody reacts with Human. Supplied as 100ug in Liquid form antibody.
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Key=thumbnail, value=/c/d/cd33_biotin-500x500.jpg
Key=url_key, value=anti-human-cd33-biotin-conjugated-flow-validated-fc01508-biotin-boster-html
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Key=image_label, value=Monocytes gated human PBMCs stained with anti-human CD33 biotin followed by SA-PE (clone MW53, red histogram). Blue histogram is for the monocytes gated PBMCs stained with IgG1 (MOPC31C) biotin conjugated isotype control followed by staining with SA-PE.
Key=small_image_label, value=Monocytes gated human PBMCs stained with anti-human CD33 biotin followed by SA-PE (clone MW53, red histogram). Blue histogram is for the monocytes gated PBMCs stained with IgG1 (MOPC31C) biotin conjugated isotype control followed by staining with SA-PE.
Key=thumbnail_label, value=Monocytes gated human PBMCs stained with anti-human CD33 biotin followed by SA-PE (clone MW53, red histogram). Blue histogram is for the monocytes gated PBMCs stained with IgG1 (MOPC31C) biotin conjugated isotype control followed by staining with SA-PE.
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Key=size, value=100ug
Key=protein_name, value=Myeloid cell surface antigen CD33
Key=gene_full_name, value=Myeloid cell surface antigen CD33
Key=uniprot_id, value=P20138
Key=applications, value=Flow Cytometry
Key=reactivity, value=Human
Key=form, value=Liquid
Key=concentration, value=0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Key=clone_number, value=Clone: WM53
Key=source_company, value=Caprico 104051, 100ug, $119
Key=isotype, value=IgG1,k
Key=molecular_weight, value=39825 MW
Key=related_product_skus, value=FC01508,FC01508-APC,FC01508-FITC,FC01508-PE,PB9932,PROTP20138,RP1105
Key=product_category, value=Primary Antibodies, Monoclonal Antibodies
Key=research_category, value=Cell Type Markers, Hematopoietic Progenitors, Immunology, Stem Cells
Key=clonality, value=Monoclonal
Key=rating_value, value=100
Key=review_count, value=20
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Key=cj_related_products, value=Myeloblastic Leukemia:PB9932|RP1105|A01508|A08907|A08907-1^Precursor T-cell Lymphoblastic Leukemia-lymphoma:EK0713
Key=description, value=Boster Bio Anti-human CD33/Siglec 3 Monoclonal Antibody Biotin Conjugated, Flow Validated (Catalog# FC01508-Biotin). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Key=short_description, value=Boster Bio Anti-human CD33/Siglec 3 Monoclonal Antibody Biotin Conjugated, Flow Validated (Catalog# FC01508-Biotin). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Key=meta_keyword, value=Anti-human CD33/Siglec 3 Monoclonal Antibody Biotin Conjugated, Flow Validated
Key=contents, value=PBS pH 7.2, 0.09% sodium azide
Key=background, value=CD33, also known as Siglec-3 (sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC-3, gp67, p67), is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells. CD33 binds sialic acids, therefore is a member of the SIGLEC family of lectins. By fluorescence in situ hybridization, CD33 is mapped to 19q13.3-q13.4.
Key=gene_name, value=CD33
Key=synonyms, value=Myeloid cell surface antigen CD33;Sialic acid-binding Ig-like lectin 3;Siglec-3;gp67;CD33;CD33;SIGLEC3;
Key=application_details, value=User needs to optimize the dilution ratio for this antibody.
Key=subcellular_localization, value= Cell membrane; Single-pass type I membrane protein.
Key=tissue_specificity, value= Monocytic/myeloid lineage cells.
Key=protein_function, value= Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase (s) via their SH2 domain (s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). .
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Introduction

The clone WM53 selectively binds with a 67kDa glycoprotein, known as CD33 having sialic acid-binding immunoglobulin-like lectin. It belongs to the ITIM (immuno-receptor tyrosine-based inhibitory motif) containing molecules able of recruiting protein tyrosine phosphatases. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.

This antibody is routinely tested by flow cytometric analysis. Flow cytometry and other applications were tested during antibody development by CapricoBio or are reported in the literature.

Application Information

Each lot of this antibody has been quality control tested by flow cytometric analysis of human PBMCs. For flow cytometric staining, the recommended use of this antibody is 0.5μg per 1x106 cells in 100μl of staining volume followed by a florescent conjugated Streptavidin. However, it is strongly suggested that the antibody reactivity be empirically titrated for optimal performance in the application of interest.

Key=competitor_equivalent_skus, value=sc 7040|sc 7041|sc 19660|sc 7039|sc 28810|sc 28811|sc 376184|sc 59020|sc 7038|sc 374450|sc 53199|sc 514119
Key=immunogen, value=Human AML cells
Key=purification, value=Protein A purified
Key=storage, value=Store at 2-8°C. Avoid repeated freeze-thaw cycles.
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