Click image to see more details
Product Info Summary
| SKU: | FC01508-PE |
|---|---|
| Size: | 100 tests, 200 tests |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-human CD33/Siglec 3 Monoclonal Antibody PE Conjugated, Flow Validated
View all Siglec-3/CD33 Antibodies
SKU/Catalog Number
FC01508-PE
Size
100 tests, 200 tests
Form
Liquid
Description
Boster Bio Anti-human CD33/Siglec 3 Monoclonal Antibody PE Conjugated, Flow Validated (Catalog# FC01508-PE). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD33/Siglec 3 Monoclonal Antibody PE Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC01508-PE)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: WM53
Isotype
IgG1,k
Immunogen
Human AML cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC01508-PE is reactive to CD33 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
39825 MW
Background of Siglec-3/CD33
CD33, also known as Siglec-3 (sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC-3, gp67, p67), is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells. CD33 binds sialic acids, therefore is a member of the SIGLEC family of lectins. By fluorescence in situ hybridization, CD33 is mapped to 19q13.3-q13.4.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC01508-PE is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
Click image to see more details
Monocytes gated human PBMCs stained with mouse anti-human CD33 PE (clone WM53, red histogram). Blue histogram is for the monocytes gated PBMCs stained with IgG1 (MOPC31C) PE conjugated isotype control.
Protein Target Info & Infographic
Gene/Protein Information For CD33 (Source: Uniprot.org, NCBI)
Gene Name
CD33
Full Name
Myeloid cell surface antigen CD33
Weight
39825 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Myeloid cell surface antigen CD33;Sialic acid-binding Ig-like lectin 3;Siglec-3;gp67;CD33;CD33;SIGLEC3; CD33 SIGLEC-3, SIGLEC3, p67 CD33 molecule myeloid cell surface CD33|CD33 (gp67)|CD33 molecule transcript|gp67|sialic acid-binding Ig-like lectin 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD33, check out the CD33 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD33: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD33/Siglec 3 Monoclonal Antibody PE Conjugated, Flow Validated (FC01508-PE)
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No publications found for FC01508-PE
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Customer Q&As
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10 Customer Q&As for Anti-human CD33/Siglec 3 Monoclonal Antibody PE Conjugated, Flow Validated
Question
Will anti-human CD33/Siglec 3 Monoclonal antibody PE Conjugated, Flow Validated FC01508-PE work for Flow Cytometry with premonocytic lymphoma?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
According to the expression profile of premonocytic lymphoma, CD33 is highly expressed in premonocytic lymphoma. So, it is likely that anti-human CD33/Siglec 3 Monoclonal antibody PE Conjugated, Flow Validated FC01508-PE will work for Flow Cytometry with premonocytic lymphoma.
Boster Scientific Support
Answered: 2020-04-20
Question
My question regarding product FC01508-PE, anti-human CD33/Siglec 3 Monoclonal antibody PE Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC01508-PE anti-human CD33/Siglec 3 Monoclonal antibody PE Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-31
Question
Our lab want to know about to test anti-human CD33/Siglec 3 Monoclonal antibody PE Conjugated, Flow Validated FC01508-PE on human premonocytic lymphoma for research purposes, then I may be interested in using anti-human CD33/Siglec 3 Monoclonal antibody PE Conjugated, Flow Validated FC01508-PE for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
The products we sell, including anti-human CD33/Siglec 3 Monoclonal antibody PE Conjugated, Flow Validated FC01508-PE, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-17
Question
Is a blocking peptide available for product anti-human CD33/Siglec 3 Monoclonal antibody PE Conjugated, Flow Validated (FC01508-PE)?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
We do provide the blocking peptide for product anti-human CD33/Siglec 3 Monoclonal antibody PE Conjugated, Flow Validated (FC01508-PE). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-19
Question
I was wanting to use your anti-human CD33/Siglec 3 Monoclonal antibody PE Conjugated, Flow Validated for Flow Cytometry for human premonocytic lymphoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human premonocytic lymphoma identification?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
It shows on the product datasheet, FC01508-PE anti-human CD33/Siglec 3 Monoclonal antibody PE Conjugated, Flow Validated has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human premonocytic lymphoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-13
Question
I see that the anti-human CD33/Siglec 3 Monoclonal antibody PE Conjugated, Flow Validated FC01508-PE works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-12-18
Question
I have attached the WB image, lot number and protocol we used for premonocytic lymphoma using anti-human CD33/Siglec 3 Monoclonal antibody PE Conjugated, Flow Validated FC01508-PE. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-01-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-16
Question
Do you have a BSA free version of anti-human CD33/Siglec 3 Monoclonal antibody PE Conjugated, Flow Validated FC01508-PE available?
Verified Customer
Verified customer
Asked: 2017-12-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-human CD33/Siglec 3 Monoclonal antibody PE Conjugated, Flow Validated FC01508-PE are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-28
Question
Would FC01508-PE anti-human CD33/Siglec 3 Monoclonal antibody PE Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Banerjee
Verified customer
Asked: 2017-04-07
Answer
You can see on the product datasheet, FC01508-PE anti-human CD33/Siglec 3 Monoclonal antibody PE Conjugated, Flow Validated as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-04-07
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for premonocytic lymphoma using anti-human CD33/Siglec 3 Monoclonal antibody PE Conjugated, Flow Validated FC01508-PE. Let me know if you need anything else.
Z. Gonzalez
Verified customer
Asked: 2013-06-20
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-06-20
