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Product Info Summary
| SKU: | A08741-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-HSD17B8 Antibody Picoband®
SKU/Catalog Number
A08741-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HSD17B8 Antibody Picoband® catalog # A08741-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-HSD17B8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08741-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HSD17B8 recombinant protein (Position: D42-D244).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08741-1 is reactive to HSD17B8 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
34 kDa
Calculated molecular weight
26.974kDa
Background of HSD17B8
Estradiol 17 beta-dehydrogenase 8 is an enzyme that in humans is encoded by the HSD17B8 gene. In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. The protein encoded by this gene is similar to Ke6 and is a member of the short-chain dehydrogenase superfamily. An alternatively spliced transcript of this gene has been detected, but the full-length nature of this variant has not been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08741-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HepG2 whole cell, human Hela whole cell, rat liver tissue, mouse liver tissue
FCM: JK cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HSD17B8 using anti-HSD17B8 antibody (A08741-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B8 antigen affinity purified polyclonal antibody (Catalog # A08741-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B8 at approximately 34 kDa. The expected band size for HSD17B8 is at 34 kDa.
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Figure 2. Flow Cytometry analysis of JK cells using anti-HSD17B8 antibody (A08741-1).
Overlay histogram showing JK cells stained with A08741-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSD17B8 Antibody (A08741-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For HSD17B8 (Source: Uniprot.org, NCBI)
Gene Name
HSD17B8
Full Name
Estradiol 17-beta-dehydrogenase 8
Weight
26.974kDa
Superfamily
short-chain dehydrogenases/reductases (SDR) family
Alternative Names
Transcription factor Sp6; Krueppel-like factor 14; SP6; KLF14 HSD17B8 D6S2245E, FABG, FABGL, H2-KE6, HKE6, KE6, RING2, SDR30C1, dJ1033B10.9 hydroxysteroid 17-beta dehydrogenase 8 estradiol 17-beta-dehydrogenase 8|17-beta-HSD 8|17-beta-hydroxysteroid dehydrogenase 8|3-ketoacyl-[acyl-carrier-protein] reductase alpha subunit|3-oxoacyl-[acyl-carrier-protein] reductase|KAR alpha subunit|estrogen 17-oxidoreductase|ke-6|protein Ke6|really interesting new gene 2 protein|short chain dehydrogenase/reductase family 30C member 1|testosterone 17-beta-dehydrogenase 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSD17B8, check out the HSD17B8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSD17B8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSD17B8 Antibody Picoband® (A08741-1)
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