Anti-Homeobox protein NANOG NANOG Antibody

Product Name

Anti-Homeobox protein NANOG NANOG Antibody

View all Nanog Antibodies

SKU/Catalog Number

A00153

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Homeobox protein NANOG NANOG Antibody (Catalog # A00153). Tested in ELISA, WB, IHC-P, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

NANOG antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Homeobox protein NANOG NANOG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00153)

Host

Rabbit

Contents

NANOG Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

NANOG antibody was raised against a 19 amino acid synthetic peptide near the center of human NANOG. The immunogen is located within amino acids 130 - 180 of NANOG.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Two alternatively spliced transcript variants have been observed.

Reactive Species

A00153 is reactive to NANOG in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

35 kDa

Calculated molecular weight

35 kDa

Background of Nanog

Expression of NANOG is required for the maintenance of pluripotency in epiblast and embryonic stem (ES) cells as well as for the ability to maintain ES self-renewal independently of LIF/Stat3. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00153 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1-2 μg/mL; ICC/IF: 20 μg/mL; IHC: 1-5 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse and rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Validation Images & Assay Conditions

Gene/Protein Information For NANOG (Source: Uniprot.org, NCBI)

Alternative Names

Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog Nanog|2410002E02Rik, EN, ENK, ecat, ecat4|Nanog homeobox|homeobox protein NANOG|ES cell-associated protein 4|early embryo specific expression NK family|early embryo specific expression NK-type homeobox protein|homeobox transcription factor Nanog

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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