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Product Info Summary
| SKU: | PA2211 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Krueppel-like factor 4 KLF4 Antibody Picoband®
SKU/Catalog Number
PA2211
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Krueppel-like factor 4 KLF4 Antibody catalog # PA2211. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Krueppel-like factor 4 KLF4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2211)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human KLF4, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2211 is reactive to KLF4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
47 kDa
Calculated molecular weight
54671 MW
Background of KLF4
Kruppel-like factor 4, also known as EZF or GKLF, is a protein that in humans is encoded by the KLF4 gene. This gene is mapped to 9q31.2. KLF4 gene encodes a member of the Kruppel family of transcription factors. This gene plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. It is required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. This gene involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2211 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: HELA Cell, A549 Cell, U20S Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-KLF4 antibody, PA2211, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: A549 Cell Lysate
Lane 3: U20S Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For KLF4 (Source: Uniprot.org, NCBI)
Gene Name
KLF4
Full Name
Krueppel-like factor 4
Weight
54671 MW
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
Krueppel-like factor 4;Epithelial zinc finger protein EZF;Gut-enriched krueppel-like factor;KLF4;EZF, GKLF; KLF4 EZF, GKLF Kruppel like factor 4 Krueppel-like factor 4|Kruppel-like factor 4 (gut)|endothelial Kruppel-like zinc finger protein|epithelial zinc finger protein EZF|gut Kruppel-like factor|gut-enriched krueppel-like factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLF4, check out the KLF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Krueppel-like factor 4 KLF4 Antibody Picoband® (PA2211)
Hello CJ!
PA2211 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Deng S,Zhang Y,Wang Y,Lu X,Jiang Q.MicroRNA-92 regulates vascular smooth muscle cell function by targeting KLF4 during vascular restenosis and injury.Int J Clin Exp Pathol.2019 Dec 1;12(12):4253-4262.PMID:31933825;PMCID:PMC6949870.
Species: Human,Mouse
PA2211 usage in article: APP:WB, SAMPLE:VSMCS, DILUTION:1:1000
Shi R,Liu L,Wang F,He Y,Niu Y,Wang C,Zhang X,Zhang X,Zhang H,Chen M,Wang Y.Downregulation of cytokeratin 18 induces cellular partial EMT and stemness through increasing EpCAM expression in breast cancer.Cell Signal.2020 Dec;76:109810.doi:10.1016/j.cellsig
Species: Human
PA2211 usage in article: APP:WB, SAMPLE:MCF-7 CELL AND MDA-MB-231 CELL, DILUTION:NA
Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901
CHIR99021 enhances Klf4 Expression through ?-Catenin Signaling and miR-7a Regulation in J1 Mouse Embryonic Stem Cells
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Krueppel-like factor 4 KLF4 Antibody Picoband®
Question
I have a question about product PA2211, anti-KLF4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2211 anti-KLF4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-07
Question
Is this PA2211 anti-KLF4 antibody reactive to the isotypes of KLF4?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
The immunogen of PA2211 anti-KLF4 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human KLF4(93-108aa RRETEEFNDLLDLDFI), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-07
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-KLF4 antibody PA2211. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-02
Question
I have attached the WB image, lot number and protocol we used for cervix carcinoma using anti-KLF4 antibody PA2211. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-20
Question
We have been able to see staining in human substantia nigra tongue. Any tips? Is anti-KLF4 antibody supposed to stain substantia nigra tongue positively?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
According to literature substantia nigra tongue does express KLF4. According to Uniprot.org, KLF4 is expressed in colonic mucosa, placenta, substantia nigra tongue, cervix lung, cervix carcinoma, liver, among other tissues. Regarding which tissues have KLF4 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231, 23186163
Cervix, and Lung, Pubmed ID: 15489334
Liver, Pubmed ID: 18655026
Substantia nigra, and Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-11-18
Question
I am interested in to test anti-KLF4 antibody PA2211 on rat cervix carcinoma for research purposes, then I may be interested in using anti-KLF4 antibody PA2211 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Johnson
Verified customer
Asked: 2019-11-12
Answer
The products we sell, including anti-KLF4 antibody PA2211, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-12
Question
I see that the anti-KLF4 antibody PA2211 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-20
Question
Is a blocking peptide available for product anti-KLF4 antibody (PA2211)?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
We do provide the blocking peptide for product anti-KLF4 antibody (PA2211). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-23
Question
I was wanting to use your anti-KLF4 antibody for WB for rat cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2018-12-13
Answer
It shows on the product datasheet, PA2211 anti-KLF4 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-13
Question
Will anti-KLF4 antibody PA2211 work on dog WB with substantia nigra tongue?
Verified Customer
Verified customer
Asked: 2017-11-17
Answer
Our lab technicians have not validated anti-KLF4 antibody PA2211 on dog. You can run a BLAST between dog and the immunogen sequence of anti-KLF4 antibody PA2211 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog substantia nigra tongue in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-17
Question
I am interested in using your anti-KLF4 antibody for transcriptional regulation of white adipocyte differentiation studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
B. Parker
Verified customer
Asked: 2017-10-31
Answer
I appreciate your inquiry. This PA2211 anti-KLF4 antibody is tested on hela cell lysate, a549 cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-10-31
Question
Do you have a BSA free version of anti-KLF4 antibody PA2211 available?
D. Yang
Verified customer
Asked: 2017-10-20
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-KLF4 antibody PA2211 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-20
Question
We are currently using anti-KLF4 antibody PA2211 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
The anti-KLF4 antibody (PA2211) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-19
Question
We were well pleased with the WB result of your anti-KLF4 antibody. However we have observed positive staining in cervix lung nucleus. using this antibody. Is that expected? Could you tell me where is KLF4 supposed to be expressed?
B. Zhang
Verified customer
Asked: 2017-04-14
Answer
Based on literature, cervix lung does express KLF4. Generally KLF4 expresses in nucleus. Regarding which tissues have KLF4 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231, 23186163
Cervix, and Lung, Pubmed ID: 15489334
Liver, Pubmed ID: 18655026
Substantia nigra, and Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-04-14
Question
Would anti-KLF4 antibody PA2211 work for WB with cervix carcinoma?
A. Johnson
Verified customer
Asked: 2015-07-23
Answer
According to the expression profile of cervix carcinoma, KLF4 is highly expressed in cervix carcinoma. So, it is likely that anti-KLF4 antibody PA2211 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2015-07-23
Question
Would PA2211 anti-KLF4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Miller
Verified customer
Asked: 2015-06-09
Answer
You can see on the product datasheet, PA2211 anti-KLF4 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-06-09
