Click image to see more details
Product Info Summary
| SKU: | A02969-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Islet 1/ISL1 Antibody Picoband®
SKU/Catalog Number
A02969-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Islet 1/ISL1 Antibody Picoband® catalog # A02969-1. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Islet 1/ISL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02969-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Islet 1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A02969-1 is reactive to ISL1 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
39036 MW
Background of Islet-1
Insulin gene enhancer protein ISL-1 is a protein that in humans is encoded by the isl1 gene. This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02969-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: PC12 whole cell, MCF-7 whole cell
IHC: human mammary cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Islet 1 using anti-Islet 1 antibody (A02969-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: PC12 whole cell lysates,
lane 2: MCF-7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Islet 1 antigen affinity purified polyclonal antibody (Catalog # A02969-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Islet 1 at approximately 39KD. The expected band size for Islet 1 is at 39KD.
Click image to see more details
Figure 2. IHC analysis of Islet 1 using anti-Islet 1 antibody (A02969-1).
Islet 1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Islet 1 Antibody (A02969-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ISL1 (Source: Uniprot.org, NCBI)
Gene Name
ISL1
Full Name
Insulin gene enhancer protein ISL-1
Weight
39036 MW
Alternative Names
Insulin gene enhancer protein ISL-1;Islet-1;ISL1; ISL1 ISLET1, Isl-1 ISL LIM homeobox 1 insulin gene enhancer protein ISL-1|islet-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ISL1, check out the ISL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ISL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Islet 1/ISL1 Antibody Picoband® (A02969-1)
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No publications found for A02969-1
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Customer Q&As
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15 Customer Q&As for Anti-Islet 1/ISL1 Antibody Picoband®
Question
I was wanting to use your anti-Islet 1/ISL1 antibody for IHC for human forebrain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human forebrain identification?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
You can see on the product datasheet, A02969-1 anti-Islet 1/ISL1 antibody has been validated for IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human forebrain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-05
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for forebrain using anti-Islet 1/ISL1 antibody A02969-1. Let me know if you need anything else.
E. Collins
Verified customer
Asked: 2020-03-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-04
Question
Is there a BSA free version of anti-Islet 1/ISL1 antibody A02969-1 available?
P. Mangal
Verified customer
Asked: 2020-01-07
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Islet 1/ISL1 antibody A02969-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-07
Question
Will anti-Islet 1/ISL1 antibody A02969-1 work for IHC with forebrain?
Verified Customer
Verified customer
Asked: 2019-02-19
Answer
According to the expression profile of forebrain, ISL1 is highly expressed in forebrain. So, it is likely that anti-Islet 1/ISL1 antibody A02969-1 will work for IHC with forebrain.
Boster Scientific Support
Answered: 2019-02-19
Question
Our lab used your anti-Islet 1/ISL1 antibody for IHC on platelet in the past. I am using human, and We are going to use the antibody for WB next. We are interested in examining platelet as well as forebrain in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-09-18
Answer
I viewed the website and datasheets of our anti-Islet 1/ISL1 antibody and it seems that A02969-1 has been validated on human in both IHC and WB. Thus A02969-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-09-18
Question
We have been able to see staining in rat testis. What should we do? Is anti-Islet 1/ISL1 antibody supposed to stain testis positively?
Verified Customer
Verified customer
Asked: 2018-08-08
Answer
From literature testis does express ISL1. From Uniprot.org, ISL1 is expressed in forebrain, pancreatic islet, testis, platelet, among other tissues. Regarding which tissues have ISL1 expression, here are a few articles citing expression in various tissues:
Pancreatic islet, Pubmed ID: 7912209
Platelet, Pubmed ID: 18088087
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-08-08
Question
Does A02969-1 anti-Islet 1/ISL1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-25
Answer
As indicated on the product datasheet, A02969-1 anti-Islet 1/ISL1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-25
Question
I am looking for to test anti-Islet 1/ISL1 antibody A02969-1 on human forebrain for research purposes, then I may be interested in using anti-Islet 1/ISL1 antibody A02969-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-12-07
Answer
The products we sell, including anti-Islet 1/ISL1 antibody A02969-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-07
Question
Is a blocking peptide available for product anti-Islet 1/ISL1 antibody (A02969-1)?
J. Collins
Verified customer
Asked: 2017-10-05
Answer
We do provide the blocking peptide for product anti-Islet 1/ISL1 antibody (A02969-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-10-05
Question
I have a question about product A02969-1, anti-Islet 1/ISL1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Roberts
Verified customer
Asked: 2017-08-24
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02969-1 anti-Islet 1/ISL1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-08-24
Question
Is this A02969-1 anti-Islet 1/ISL1 antibody reactive to the isotypes of ISL1?
M. Evans
Verified customer
Asked: 2016-10-05
Answer
The immunogen of A02969-1 anti-Islet 1/ISL1 antibody is A synthetic peptide corresponding to a sequence in the middle region of human Islet 1 (118-161aa DEFALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAE), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-10-05
Question
I see that the anti-Islet 1/ISL1 antibody A02969-1 works with IHC, what is the protocol used to produce the result images on the product page?
W. Wu
Verified customer
Asked: 2016-08-11
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-08-11
Question
My team were happy with the WB result of your anti-Islet 1/ISL1 antibody. However we have observed positive staining in forebrain nucleus. using this antibody. Is that expected? Could you tell me where is ISL1 supposed to be expressed?
A. Collins
Verified customer
Asked: 2016-07-19
Answer
From what I have seen in literature, forebrain does express ISL1. Generally ISL1 expresses in nucleus. Regarding which tissues have ISL1 expression, here are a few articles citing expression in various tissues:
Pancreatic islet, Pubmed ID: 7912209
Platelet, Pubmed ID: 18088087
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-07-19
Question
See attached the WB image, lot number and protocol we used for forebrain using anti-Islet 1/ISL1 antibody A02969-1. Please let me know if you require anything else.
C. Banerjee
Verified customer
Asked: 2014-07-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-07-10
Question
We are currently using anti-Islet 1/ISL1 antibody A02969-1 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on primate tissues as well?
G. Jackson
Verified customer
Asked: 2014-04-28
Answer
The anti-Islet 1/ISL1 antibody (A02969-1) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-04-28
