Click image to see more details
Product Info Summary
| SKU: | M00253-1 |
|---|---|
| Size: | 50 µg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-HO-1 Monoclonal Antibody (HO-1-2)
View all HO-1/HMOX1/HSP32 Antibodies
SKU/Catalog Number
M00253-1
Size
50 µg
Form
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Description
Boster Bio Anti-HO-1 Monoclonal Antibody (HO-1-2) catalog # M00253-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HO-1 Monoclonal Antibody (HO-1-2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00253-1)
Host
Mouse
Contents
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Clonality
Monoclonal
Clone Number
HO-1-2
Isotype
IgG
Immunogen
Native rat HO-1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M00253-1 is reactive to Hmox1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
68 kDa
Calculated molecular weight
154537 MW
Background of HO-1/HMOX1/HSP32
Anti Apolipoprotein C-III antibody recognizes the gene product of APOC. Apolipoprotein C-III is a protein component of very low density lipoprotein (VLDL). APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to inhibit hepatic uptake of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia. This antibody is suitable for cardiovascular research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00253-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1:100)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
Click image to see more details
Flow cytometry analysis of human lung cancer A2 cells stained using isotype control antibody (left) and HO-1 (Hsp32) mAb (HO -1-2) (right).
Click image to see more details
Figure 1. Western blot analysis of Hmox1 using anti-Hmox1 antibody (M00253-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hmox1 antigen affinity purified polyclonal antibody (Catalog # M00253-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for Hmox1.
Click image to see more details
Figure 3. IHC analysis of Hmox1 using anti-Hmox1 antibody (M00253-1).
Hmox1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Hmox1 Antibody (M00253-1) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Hmox1 using anti-Hmox1 antibody (M00253-1).
Hmox1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Hmox1 Antibody (M00253-1) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Hmox1 (Source: Uniprot.org, NCBI)
Gene Name
Hmox1
Full Name
Heme oxygenase 1
Weight
154537 MW
Superfamily
heme oxygenase family
Alternative Names
Apolipoprotein C-III, ApoC-III, Apo-CIII, Apolipoprotein C3, Apolipoprotein C III HMOX1 HMOX1D, HO-1, HSP32, bK286B10 heme oxygenase 1 heme oxygenase 1|heat shock protein, 32-kD|heme oxygenase (decycling) 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Hmox1, check out the Hmox1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Hmox1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HO-1 Monoclonal Antibody (HO-1-2) (M00253-1)
Hello CJ!
M00253-1 has been cited in 19 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Dong H,Hao L,Zhang W,Zhong W,Guo W,Yue R,Sun X,Zhou Z.Activation of AhR-NQO1 signaling pathway protects against alcohol-induced liver injury by improving redox balance.Cell Mol Gastroenterol Hepatol.2021 May 31:S2352-345X(21)00102-8.doi:10.1016/j.jcmgh.2021.05.013.Epub ahead of print.PMID:34082111.
Species: Human,Mouse
M00253-1 usage in article: APP:WB, SAMPLE:LIVER TISSUE AND HEPA-1C1C7 CELL, DILUTION:NA
Sun S,Zhang J,Li H,Du Y,Li S,Li A,Suo X,Wang Y,Sun Q.Anti-inflammatory activity of the water extract of Chloranthus serratus roots in LPS-stimulated RAW264.7 cells mediated by the Nrf2/HO-1, MAPK and NF-κB signaling pathways.J Ethnopharmacol.2021 Jan 25:1
Species: Mouse
M00253-1 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:1:400
Li D,Bai X,Jiang Y,Cheng Y.Butyrate alleviates PTZ-induced mitochondrial dysfunction, oxidative stress and neuron apoptosis in mice via Keap1/Nrf2/HO-1 pathway.Brain Res Bull.2020 Dec 28;168:25-35.doi:10.1016/j.brainresbull.2020.12.009.Epub ahead of print
Species: Mouse
M00253-1 usage in article: APP:WB, SAMPLE:HIPPOCAMPUS, DILUTION:1:200
Sun S,Wang Y,Du Y,Sun Q,He L,Zhu E,Li J. Oxidative stress-mediated hepatotoxicity in rats induced by ethanol extracts of different parts of Chloranthus serratus.Pharm Biol.2020 Dec;58(1):1277-1289.doi:10.1080/ n13880209.2020.1859552.PMID: 33355514.
Species: Rat
M00253-1 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:1:400
Role of heme oxygenase-1/carbon monoxide system in pulmonary ischemia-reperfusion injury
Heme Oxygenase-1 Promotes Delayed Wound Healing in Diabetic Rats
Endogenous carbon monoxide downregulates hepatic cystathionine-?-lyase in rats with liver cirrhosis
Hydrogen sulfide accelerates wound healing in diabetic rats
Octreotide attenuates liver fibrosis by inhibiting hepatic heme oxygenase-1 expression
Evidence for a therapeutic effect of Braintone on ischemic brain damage
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-HO-1 Monoclonal Antibody (HO-1-2) ?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-HO-1 Monoclonal Antibody (HO-1-2)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-HO-1 Monoclonal Antibody (HO-1-2)
Question
We have been able to see staining in rat layer of synovial tissue. Any tips? Is anti-HO-1 Monoclonal antibody (HO-1-2) supposed to stain layer of synovial tissue positively?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
According to literature layer of synovial tissue does express HMOX1. According to Uniprot.org, HMOX1 is expressed in layer of synovial tissue, foreskin, cervix carcinoma, among other tissues. Regarding which tissues have HMOX1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 20068231
Foreskin, Pubmed ID: 2911585
Boster Scientific Support
Answered: 2020-03-06
Question
My team were satisfied with the WB result of your anti-HO-1 Monoclonal antibody (HO-1-2) . However we have been able to see positive staining in layer of synovial tissue microsome. using this antibody. Is that expected? Could you tell me where is HMOX1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-03-22
Answer
Based on literature, layer of synovial tissue does express HMOX1. Generally HMOX1 expresses in microsome. Regarding which tissues have HMOX1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 20068231
Foreskin, Pubmed ID: 2911585
Boster Scientific Support
Answered: 2019-03-22
Question
I would like using your anti-HO-1 Monoclonal antibody (HO-1-2) for regulation of dna-binding transcription factor activity studies. Has this antibody been tested with western blotting on cancer a2 cells? We would like to see some validation images before ordering.
A. Taylor
Verified customer
Asked: 2018-06-26
Answer
Thanks for your inquiry. This M00253-1 anti-HO-1 Monoclonal antibody (HO-1-2) is tested on cancer a2 cells. It is guaranteed to work for Flow Cytometry, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-06-26
Question
We are currently using anti-HO-1 Monoclonal antibody (HO-1-2) M00253-1 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
A. Huang
Verified customer
Asked: 2018-05-31
Answer
The anti-HO-1 Monoclonal antibody (HO-1-2) (M00253-1) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-31
Question
We bought anti-HO-1 Monoclonal antibody (HO-1-2) for Flow Cytometry on foreskin in the past. I am using human, and We intend to use the antibody for IHC next. I am looking for examining foreskin as well as cervix carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2017-09-19
Answer
I have checked the website and datasheets of our anti-HO-1 Monoclonal antibody (HO-1-2) and I see that M00253-1 has been tested on human in both Flow Cytometry and IHC. Thus M00253-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-09-19
