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Product Info Summary
| SKU: | EK7112 |
|---|---|
| Size: | 96 wells/kit |
| Reactive Species: | Human, Rat |
| Application: | ELISA |
| Sample Types: | Cell Lysates, Tissue, Serum, Cyst fluid |
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Product info
Product Name
HO-1 ELISA Kit
See all HO-1/HMOX1/HSP32 products
SKU/Catalog Number
EK7112
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Colorimetric detection of HO-1. 96 wells/kit.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
HO-1 ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7112)
Clonality of Antibodies
See Datasheet for details
Sensitivity
0.21 ng/ml
Assay Range
0.781 - 50 ng/ml
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7112 is reactive to HO-1 in Human, Rat samples
Validated Sample Types
Cell Lysates, Tissue, Serum, Cyst fluid
Application Guarantee
EK7112 is guaranteed for ELISA in Human, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Description | Quantity |
| Anti-HO-1 Immunoassay Plate | 1 Plate |
| 5X HO-1 Extraction Reagent | 1 vial/10 ml |
| Recombinant HO-1 Standard | 2 vials |
| Standard and Sample Diluent | 1 vial/ 50 ml |
| 10X Wash Buffer Concentrate | 1 vial/100 ml |
| Anti-HO-1 Biotinylated Antibody Concentrate | 1 vial/150 μl |
| Anti-HO-1 Biotinylated Antibody Diluent | 1 vial/ 13 ml |
| Streptavidin: HRP Concentrate | 1 vial/150 μl |
| Streptavidin: HRP Diluent | 1 vial/ 13 ml |
| TMB Substrate | 1 vial/ 13 ml |
| Stop Solution | 1 vial/ 13 ml |
| Pre-treatment Buffer | 1 vial/ 13 ml |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Data Example Images
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Typical Standard Curve for the HO-1 ELISA Kit (Enzyme-Linked Immunosorbent Assay)K7112
Assay Type: Sandwich ELISA.
Detection Method: Colorimetric Assay.
Assay Range: 0.781 50 ng/mL.
Specific Publications For HO-1 ELISA Kit (EK7112)
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