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Product Info Summary
| SKU: | M00253-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Bovine, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat |
| Host: | Mouse |
| Application: | ELISA, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HO-1 Monoclonal Antibody
View all HO-1/HMOX1/HSP32 Antibodies
SKU/Catalog Number
M00253-2
Size
100ug
Form
liquid
Description
Boster Bio Anti-HO-1 Monoclonal Antibody catalog # M00253-2. Tested in ELISA, IP, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HO-1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00253-2)
Host
Mouse
Contents
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Clonality
Monoclonal
Clone Number
1F12-A6
Isotype
IgG1 Kappa
Immunogen
Human HO-1 synthetic peptide, amino acids 1-30
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects 32kDa. Does not cross-react with HO-2.
Reactive Species
M00253-2 is reactive to HO-1 in Bovine, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
32.819kDa
Background of HO-1/HMOX1/HSP32
Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00253-2 is guaranteed for ELISA, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Validation Images & Assay Conditions
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HO-1 Monoclonal Antibody, Clone 1F12-A6 (M00253-2) . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody (M00253-2) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cell-cell border staining in epidermis, punctuate nuclear staining. .
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Figure 2. IHC analysis of HMOX1 using anti-HMOX1 antibody (M00253-2).
HMOX1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HMOX1 Antibody (M00253-2) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
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Figure 4. Western blot analysis of HMOX1 using anti-HMOX1 antibody (M00253-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMOX1 antigen affinity purified polyclonal antibody (Catalog # M00253-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for HMOX1.
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HO-1 Monoclonal Antibody, Clone 1F12-A6 (M00253-2) . Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody (M00253-2) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Microsome. Endoplasmic reticulum. Localizes to the nucleus upon hypoxia. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HO-1 Antibody. (C) Composite.
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HO-1 Monoclonal Antibody, Clone 1F12-A6 (M00253-2) . Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody (M00253-2) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Microsome. Endoplasmic reticulum. Localizes to the nucleus upon hypoxia. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HO-1 Antibody. (C) Composite.
Protein Target Info & Infographic
Gene/Protein Information For HO-1 (Source: Uniprot.org, NCBI)
Gene Name
HO-1
Full Name
Heme oxygenase 1
Weight
32.819kDa
Superfamily
heme oxygenase family
Alternative Names
HSP32, HMOX1, Heme oxygenase 1, HO, HO1, , 32 kD, bK286B10, D8Wsu38e, heat shock protein 32 kD, heat shock protein 32kD, Heat shock protein, Heme oxygenase (decycling) 1, Heme oxygenase 1, Hemox, HMOX 1, Hmox, Hmox1, HMOX1_HUMAN, HO 1, HO, HO-1, HO1, Hsp32 HMOX1 HMOX1D, HO-1, HSP32, bK286B10 heme oxygenase 1 heme oxygenase 1|heat shock protein, 32-kD|heme oxygenase (decycling) 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HO-1, check out the HO-1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HO-1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HO-1 Monoclonal Antibody (M00253-2)
Hello CJ!
M00253-2 has been cited in 24 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Inactivated pseudomonas aeruginosa protects against myocardial ischemia reperfusion injury via Nrf2 and HO‑1
Rhodiola rosea L. Attenuates Cigarette Smoke and Lipopolysaccharide-Induced COPD in Rats via Inflammation Inhibition and Antioxidant and Antifibrosis Pathways
Polyphenol-Rich Extract from Litchi chinensis Seeds Alleviates Hypertension-Induced Renal Damage in Rats
Improvement of Cisplatin-induced renal dysfunction by Schisandra chinensis stems via anti-inflammation and anti-apoptosis effects
Dong H,Hao L,Zhang W,Zhong W,Guo W,Yue R,Sun X,Zhou Z.Activation of AhR-NQO1 signaling pathway protects against alcohol-induced liver injury by improving redox balance.Cell Mol Gastroenterol Hepatol.2021 May 31:S2352-345X(21)00102-8.doi:10.1016/j.jcmgh.2021.05.013.Epub ahead of print.PMID:34082111.
Species: Human,Mouse
M00253-2 usage in article: APP:WB, SAMPLE: LIVER TISSUE AND HEPA-1C1C7 CELL, DILUTION:NA
Huanyue Cui,Xueying Liu,Jin Zhang,Ke Zhang,Dahong Yao,Shi Dong,Shushu Feng,Lu Yang, Yuyao Li,Hangyu Wang,Jian Huang,Jinhui Wang,"Rhodiola rosea L. Attenuates Cigarette Smoke and Lipopolysaccharide-Induced COPD in Rats via Inflammation Inhibition and Antioxidant and Antifibrosis Pathways",Evidence-Based Complementary and Alternative Medicine,vol. 2021,Article ID 6103158,18 pages,2021.https://doi.org/10.1155/2021/6103158
Species: Human,Rat
M00253-2 usage in article: APP:WB, SAMPLE:LUNG TISSUE, DILUTION:1:500
Sun S,Zhang J,Li H,Du Y,Li S,Li A,Suo X,Wang Y,Sun Q.Anti-inflammatory activity of the water extract of Chloranthus serratus roots in LPS-stimulated RAW264.7 cells mediated by the Nrf2/HO-1, MAPK and NF-κB signaling pathways.J Ethnopharmacol.2021 Jan 25:1
Species: Mouse
M00253-2 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:1:400
Li D,Bai X,Jiang Y,Cheng Y.Butyrate alleviates PTZ-induced mitochondrial dysfunction, oxidative stress and neuron apoptosis in mice via Keap1/Nrf2/HO-1 pathway.Brain Res Bull.2020 Dec 28;168:25-35.doi:10.1016/j.brainresbull.2020.12.009.Epub ahead of print
Species: Mouse
M00253-2 usage in article: APP:WB, SAMPLE:HIPPOCAMPUS, DILUTION:1:200
Sun S,Wang Y,Du Y,Sun Q,He L,Zhu E,Li J. Oxidative stress-mediated hepatotoxicity in rats induced by ethanol extracts of different parts of Chloranthus serratus.Pharm Biol.2020 Dec;58(1):1277-1289.doi:10.1080/ n13880209.2020.1859552.PMID: 33355514.
Species: Rat
M00253-2 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:1:400
Role of heme oxygenase-1/carbon monoxide system in pulmonary ischemia-reperfusion injury
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-HO-1 Monoclonal Antibody
Question
My question regarding product M00253-2, anti-HO-1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00253-2 anti-HO-1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-19
Question
Is this M00253-2 anti-HO-1 Monoclonal antibody reactive to the isotypes of HMOX1?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
The immunogen of M00253-2 anti-HO-1 Monoclonal antibody is Human HO-1 synthetic peptide, amino acids 1-30. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-21
Question
I was wanting to use your anti-HO-1 Monoclonal antibody for WB for mouse foreskin on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse foreskin identification?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
You can see on the product datasheet, M00253-2 anti-HO-1 Monoclonal antibody has been validated for ELISA, IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse foreskin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-05
Question
I see that the anti-HO-1 Monoclonal antibody M00253-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-02
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for foreskin using anti-HO-1 Monoclonal antibody M00253-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-28
Question
Our lab used your anti-HO-1 Monoclonal antibody for ELISA on cervix carcinoma a few years ago. I am using mouse, and We want to use the antibody for WB next. I am interested in examining cervix carcinoma as well as layer of synovial tissue in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
I have checked the website and datasheets of our anti-HO-1 Monoclonal antibody and it seems that M00253-2 has been tested on mouse in both ELISA and WB. Thus M00253-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-04
Question
I am interested in to test anti-HO-1 Monoclonal antibody M00253-2 on mouse foreskin for research purposes, then I may be interested in using anti-HO-1 Monoclonal antibody M00253-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
F. Miller
Verified customer
Asked: 2019-04-09
Answer
The products we sell, including anti-HO-1 Monoclonal antibody M00253-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-09
Question
Here is the WB image, lot number and protocol we used for foreskin using anti-HO-1 Monoclonal antibody M00253-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-03-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-07
Question
My team were well pleased with the WB result of your anti-HO-1 Monoclonal antibody. However we have seen positive staining in layer of synovial tissue microsome. using this antibody. Is that expected? Could you tell me where is HMOX1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-01-14
Answer
Based on literature, layer of synovial tissue does express HMOX1. Generally HMOX1 expresses in microsome. Regarding which tissues have HMOX1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 20068231
Foreskin, Pubmed ID: 2911585
Boster Scientific Support
Answered: 2019-01-14
Question
Would anti-HO-1 Monoclonal antibody M00253-2 work for WB with foreskin?
H. Jones
Verified customer
Asked: 2018-11-30
Answer
According to the expression profile of foreskin, HMOX1 is highly expressed in foreskin. So, it is likely that anti-HO-1 Monoclonal antibody M00253-2 will work for WB with foreskin.
Boster Scientific Support
Answered: 2018-11-30
Question
My question regards using your anti-HO-1 Monoclonal antibody for endothelial cell proliferation studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
D. Roberts
Verified customer
Asked: 2018-04-19
Answer
Thanks for your inquiry. This M00253-2 anti-HO-1 Monoclonal antibody is tested on hacat cells, hela cells. It is guaranteed to work for ELISA, IP, IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-04-19
Question
Would M00253-2 anti-HO-1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Wu
Verified customer
Asked: 2018-02-07
Answer
As indicated on the product datasheet, M00253-2 anti-HO-1 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-07
Question
Is a blocking peptide available for product anti-HO-1 Monoclonal antibody (M00253-2)?
Verified Customer
Verified customer
Asked: 2017-05-24
Answer
We do provide the blocking peptide for product anti-HO-1 Monoclonal antibody (M00253-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-05-24
Question
Is there a BSA free version of anti-HO-1 Monoclonal antibody M00253-2 available?
D. Miller
Verified customer
Asked: 2016-05-13
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-HO-1 Monoclonal antibody M00253-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-05-13
Question
We have been able to see staining in mouse layer of synovial tissue. Are there any suggestions? Is anti-HO-1 Monoclonal antibody supposed to stain layer of synovial tissue positively?
M. Brown
Verified customer
Asked: 2013-12-04
Answer
Based on literature layer of synovial tissue does express HMOX1. Based on Uniprot.org, HMOX1 is expressed in layer of synovial tissue, foreskin, cervix carcinoma, among other tissues. Regarding which tissues have HMOX1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 20068231
Foreskin, Pubmed ID: 2911585
Boster Scientific Support
Answered: 2013-12-04
Question
We are currently using anti-HO-1 Monoclonal antibody M00253-2 for mouse tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
J. Dhar
Verified customer
Asked: 2013-04-26
Answer
The anti-HO-1 Monoclonal antibody (M00253-2) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-04-26
