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Product Info Summary
| SKU: | EK1154 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Kininogen-1 / KNG1 / HMW Kininogen ELISA Kit PicoKine®
SKU/Catalog Number
EK1154
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Kininogen-1 / KNG1 / HMW Kininogen ELISA Kit PicoKine® (96 Tests). Quantitate Human KNG1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Kininogen-1 / KNG1 / HMW Kininogen ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1154)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q19-S644
Sensitivity
<20 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1154 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1154 is reactive to KNG1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1154 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of KNG1
Kininogen-1 (KNG1), also known as alpha-2-thiol proteinase inhibitor, Williams-Fitzgerald-Flaujeac factor or the HMWK-kallikrein factor, is a protein that in humans is encoded by the KNG1 gene. It is mapped to 3q27.3. The KNG1 gene uses alternative splicing to generate two different proteins – high – molecular - weight kininogen (HMWK) and low - molecular- weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. In addition to that, KNG1 is a constituent of the blood coagulation system as well as the kinin-kallikrein system.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1154-CAP | Anti-Human KNG1 Pre-coated 96-well strip microplate | 1 |
| EK1154-ST | Human KNG1 Standard | 2 vials, 50 ng/tube |
| EK1154-DA | Human KNG1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.051 | 0.101 | 0.134 | 0.243 | 0.439 | 0.829 | 1.482 | 2.072 |
Data Example Images
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Human Kininogen-1/KNG1 ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect KNG1, Dilution ratio of 1:500, concentration in serum and plasma is around 600 ng/ml..
Some articles we found to cite concentrations of KNG1 in samples: 23894665 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Kininogen-1 / KNG1 / HMW Kininogen ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 221 | 658 | 3779 | 249 | 672 | 4128 |
| Standard deviation | 10.83 | 33.56 | 219.18 | 17.68 | 51.07 | 321.98 |
| CV (%) | 4.9% | 5.1% | 5.8% | 7.1% | 7.6% | 7.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of KNG1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 221 | 236 | 257 | 237 | 237 | 12.79 | 5.3% |
| Sample 2 | 658 | 655 | 603 | 647 | 640 | 22.16 | 3.4% |
| Sample 3 | 3779 | 3418 | 3836 | 3710 | 3685 | 160.89 | 4.3% |
Protein Target Info & Infographic
Gene/Protein Information For KNG1 (Source: Uniprot.Org, NCBI)
Gene Name
KNG1
Full Name
Kininogen-1
Weight
71957 MW
Alternative Names
Kininogen-1;Alpha-2-thiol proteinase inhibitor;Fitzgerald factor;High molecular weight kininogen;HMWK;Williams-Fitzgerald-Flaujeac factor;Kininogen-1 heavy chain;T-kinin;Ile-Ser-Bradykinin;Bradykinin;Kallidin I;Lysyl-bradykinin;Kallidin II;Kininogen-1 light chain;Low molecular weight growth-promoting factor;KNG1;BDK, KNG; KNG1 BDK, BK, HMWK, KNG kininogen 1 kininogen-1|alpha-2-thiol proteinase inhibitor|bradykinin|fitzgerald factor|high molecular weight kininogen|williams-Fitzgerald-Flaujeac factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on KNG1, check out the KNG1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for KNG1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Kininogen-1 / KNG1 / HMW Kininogen ELISA Kit PicoKine® (EK1154)
Hello CJ!
EK1154 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Comprehensive Proteomic Profiling of Patients’ Tears Identifies Potential Biomarkers for the Traumatic Vegetative State
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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8 Customer Q&As for Human Kininogen-1 / KNG1 / HMW Kininogen ELISA Kit PicoKine®
Question
Q: how to proceed with the analysis of ELISA data? I have obtained KNG1 level in plasma.
H. Liu
Verified customer
Asked: 2020-04-26
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-04-26
Question
Q: which procedure should I follow in order to thaw whole blood sample for KNG1 ELISA after freezing?
T. Turner
Verified customer
Asked: 2020-02-16
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test KNG1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-02-16
Question
Q: What is the optimal O.D. value for KNG1 ELISA kit? I used your KNG1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain KNG1 even though the O.D. values are not very high?
P. Anderson
Verified customer
Asked: 2019-12-03
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this KNG1 ELISA kit to have sensitivity of 20pg/ml, that means the minimum amount of KNG1 that can be declared/interpreted as positive by the above standard is 20pg/ml.
Boster Scientific Support
Answered: 2019-12-03
Question
Will EK1154 work on human urine sample?
Verified customer
Asked: 2019-10-29
Answer
We are uncertain if the Human Kininogen-1 / KNG1 / HMW Kininogen ELISA Kit PicoKine® (EK1154) is able to work on human urine samples. Please try and run a pilot test. In theory, if the expression level of the target protein in the urine samples falls in the assay range of the kit, the kit should be able to detect the protein in the sample.
Boster Scientific Support
Answered: 2019-10-29
Question
Q: if the enzyme conjugated KNG1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the KNG1 antigen?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-06-06
Question
Could you tell us the epitope of the antibodies in this kit? If not the epitope, please tell us the immnogen of the antibodies in this kit. Could you tell us if this kit can detect both Intact KNG1 and cleaved kininogen (Ex. high-molecular-weight kininogen, low-molecular-weight kininogen and bradykinin)?
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
Please see the following answers for your questions. We can not provide the epitope information of the antibodies in this kit. We didn't do epitope mapping of the antibodies. Immunogen sequence of both the capture antibody and detection antibody is Q19-S644. EK1154 is able to detect both Intact KNG1 and cleaved kininogen.
Boster Scientific Support
Answered: 2019-04-17
Question
Q: Can KNG1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Y. Chen
Verified customer
Asked: 2017-01-05
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2017-01-05
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring KNG1 using the EK1154 Human KNG1 Picokine® ELISA Kit?
G. Varma
Verified customer
Asked: 2016-12-22
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2016-12-22
