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Product Info Summary
| SKU: | PB9212 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband®
View all HO-1/HMOX1/HSP32 Antibodies
SKU/Catalog Number
PB9212
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband® catalog # PB9212. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9212)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HMOX1 recombinant protein (Position: M1-M288). Human HMOX1 shares 82% and 80% amino acid (aa) sequences identity with mouse and rat HMOX1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9212 is reactive to HMOX1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
33 kDa
Calculated molecular weight
32819 MW
Background of HO-1/HMOX1/HSP32
HMOX1 (heme oxygenase (decycling) 1), also known as HO-1, is a human gene that encodes for the enzyme heme oxygenase 1. It is an essential enzyme in heme catabolism, it cleaves heme to form biliverdin. HMOX1 belongs to the heme oxygenase family. The HMOX1 gene is located on the long (q) arm of chromosome 22 at position 12.3, from base pair 34,101,636 to base pair 34,114,748. HMOX1, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. HMOX1 activity is induced by its substrate heme and by various nonheme substances.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9212 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Positive Control
WB: human A549 whole cell, human Hela whole cell
IHC: human tonsil tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 3. Western blot analysis of heme oxygenase 1/HMOX1 using anti-heme oxygenase 1/HMOX1 antibody (PB9212).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 1x PBS +0.2% Tween-20 + 10% nonfat dry milk for 1.5 hour at RT. The membrane was incubated with rabbit anti-heme oxygenase 1/HMOX1 antibody (PB9212) at 0.5 μg/mL overnight at room temperature for 3 hours, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a HRP-conjugated anti rabbit antibodies at 4℃ for 12 hours. The signal is developed using an Enhanced chemiluminescence (Thermo Fisher) for one minute. A specific band was detected for heme oxygenase 1/HMOX1 at approximately 32 kDa. The expected band size for heme oxygenase 1/HMOX1 is at 32 kDa.
Click image to see more details
Figure 1. Western blot analysis of HMOX1 using anti-HMOX1 antibody (PB9212).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMOX1 antigen affinity purified polyclonal antibody (Catalog # PB9212) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HMOX1 at approximately 33 kDa. The expected band size for HMOX1 is at 33 kDa.
Click image to see more details
Figure 2. IHC analysis of HMOX1 using anti-HMOX1 antibody (PB9212).
HMOX1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMOX1 Antibody (PB9212) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For HMOX1 (Source: Uniprot.org, NCBI)
Gene Name
HMOX1
Full Name
Heme oxygenase 1
Weight
32819 MW
Superfamily
heme oxygenase family
Alternative Names
Heme oxygenase 1;HO-1;1.14.99.3;HMOX1;HO, HO1; HMOX1 HMOX1D, HO-1, HSP32, bK286B10 heme oxygenase 1 heme oxygenase 1|heat shock protein, 32-kD|heme oxygenase (decycling) 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HMOX1, check out the HMOX1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HMOX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband® (PB9212)
Hello CJ!
PB9212 has been cited in 25 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Myricetin ameliorates brain injury and neurological deficits via Nrf2 activation after experimental stroke in middle-aged rats
Nifedipine inhibits oxidative stress and ameliorates osteoarthritis by activating the nuclear factor erythroid-2-related factor 2 pathway
Fucoxanthin regulates Nrf2 signaling to decrease oxidative stress and improves renal fibrosis depending on Sirt1 in HG-induced GMCs and STZ-induced diabetic rats
Heme oxygenase-1 expression in rats with acute lung rejection and implication
Sun S,Wang Y,Du Y,Sun Q,He L,Zhu E,Li J. Oxidative stress-mediated hepatotoxicity in rats induced by ethanol extracts of different parts of Chloranthus serratus.Pharm Biol.2020 Dec;58(1):1277-1289.doi:10.1080/ n13880209.2020.1859552.PMID: 33355514.
Species: Rat
PB9212 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:1:400
Role of heme oxygenase-1/carbon monoxide system in pulmonary ischemia-reperfusion injury
The Natural Flavone Acacetin Confers Cardiomyocyte Protection Against Hypoxia/Reoxygenation Injury via AMPK-Mediated Activation of Nrf2 Signaling Pathway
The apolipoprotein A?I mimetic peptide, D?4F, restrains neointimal formation through heme oxygenase?1 up?regulation
Heme Oxygenase-1 Promotes Delayed Wound Healing in Diabetic Rats
Endogenous carbon monoxide downregulates hepatic cystathionine-?-lyase in rats with liver cirrhosis
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Customer Reviews
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1 Reviews For Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband®
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The signal was very strong and clean!--Chi Li, MEDICINE, UNIVERSITY OF LOUISVILLE, PROFESSOR
Excellent
Source: Biocompare.com
| Applications | Western Blot |
|---|---|
| Sample | Whole cell extract of pancreatic tumor cell MIA PaACa2 |
| Detection | Enhanced chemiluminescence |
"Thirty micrograms of whole cell extract of pancreatic tumor cell MIA PaCa-2 were loaded on a 4-12% Bis-Tris gel. The membrane was blotted with 1xPBS + 0.2% Tween-20 + 10% non-fat dry milk. The membrane was incubated with Anti-HMOX1 Antibody (PB9212) at the concentration of 200 ng/ml at room temperature for 3 hours. The membrane was then blotted with HRP-conjugated anti-rabbit secondary antibodies at 4oC overnight. The band was detected using regular ECL with 1 minute exposure. A major band with 32 kD was detected."
Chi Li, University of Louisville
Verified customer
Submitted 2016-10-20
Customer Q&As
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16 Customer Q&As for Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband®
Question
Is a blocking peptide available for product anti-Heme Oxygenase 1/HMOX1 antibody (PB9212)?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
We do provide the blocking peptide for product anti-Heme Oxygenase 1/HMOX1 antibody (PB9212). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-17
Question
I would like to test anti-Heme Oxygenase 1/HMOX1 antibody PB9212 on rat cervix carcinoma for research purposes, then I may be interested in using anti-Heme Oxygenase 1/HMOX1 antibody PB9212 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Wu
Verified customer
Asked: 2020-02-10
Answer
The products we sell, including anti-Heme Oxygenase 1/HMOX1 antibody PB9212, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-10
Question
We are currently using anti-Heme Oxygenase 1/HMOX1 antibody PB9212 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
The anti-Heme Oxygenase 1/HMOX1 antibody (PB9212) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-28
Question
I see that the anti-Heme Oxygenase 1/HMOX1 antibody PB9212 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-15
Question
Does anti-Heme Oxygenase 1/HMOX1 antibody PB9212 work on horse WB with layer of synovial tissue?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
Our lab technicians have not tested anti-Heme Oxygenase 1/HMOX1 antibody PB9212 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Heme Oxygenase 1/HMOX1 antibody PB9212 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse layer of synovial tissue in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-25
Question
Is this PB9212 anti-Heme Oxygenase 1/HMOX1 antibody reactive to the isotypes of HMOX1?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
The immunogen of PB9212 anti-Heme Oxygenase 1/HMOX1 antibody is E.coli-derived human HMOX1 recombinant protein (Position: M1-M288). Human HMOX1 shares 82% and 80% amino acid (aa) sequences identity with mouse and rat HMOX1, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-27
Question
Will PB9212 anti-Heme Oxygenase 1/HMOX1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
It shows on the product datasheet, PB9212 anti-Heme Oxygenase 1/HMOX1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-09
Question
I was wanting to use your anti-Heme Oxygenase 1/HMOX1 antibody for WB for rat cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat cervix carcinoma identification?
V. Krishna
Verified customer
Asked: 2018-09-03
Answer
It shows on the product datasheet, PB9212 anti-Heme Oxygenase 1/HMOX1 antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-03
Question
We have seen staining in human cervix carcinoma. What should we do? Is anti-Heme Oxygenase 1/HMOX1 antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2018-06-25
Answer
According to literature cervix carcinoma does express HMOX1. According to Uniprot.org, HMOX1 is expressed in layer of synovial tissue, foreskin, cervix carcinoma, among other tissues. Regarding which tissues have HMOX1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 20068231
Foreskin, Pubmed ID: 2911585
Boster Scientific Support
Answered: 2018-06-25
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Heme Oxygenase 1/HMOX1 antibody PB9212. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-03-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-08
Question
I would like using your anti-Heme Oxygenase 1/HMOX1 antibody for positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-10-16
Answer
We appreciate your inquiry. This PB9212 anti-Heme Oxygenase 1/HMOX1 antibody is tested on human placenta tissue, tissue lysate, rat spleen tissue, a549 whole cell lysate, panc whole cell lysate, hela whole cell lysate. It is guaranteed to work for WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-10-16
Question
I have a question about product PB9212, anti-Heme Oxygenase 1/HMOX1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-06-14
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9212 anti-Heme Oxygenase 1/HMOX1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-14
Question
Is there a BSA free version of anti-Heme Oxygenase 1/HMOX1 antibody PB9212 available?
D. Bhatt
Verified customer
Asked: 2015-02-24
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Heme Oxygenase 1/HMOX1 antibody PB9212 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-02-24
Question
Does anti-Heme Oxygenase 1/HMOX1 antibody PB9212 work for WB with cervix carcinoma?
R. Edwards
Verified customer
Asked: 2015-02-18
Answer
According to the expression profile of cervix carcinoma, HMOX1 is highly expressed in cervix carcinoma. So, it is likely that anti-Heme Oxygenase 1/HMOX1 antibody PB9212 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2015-02-18
Question
Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-Heme Oxygenase 1/HMOX1 antibody PB9212. Please let me know if you require anything else.
D. Carter
Verified customer
Asked: 2014-05-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-05-27
Question
My team were well pleased with the WB result of your anti-Heme Oxygenase 1/HMOX1 antibody. However we have seen positive staining in layer of synovial tissue microsome. using this antibody. Is that expected? Could you tell me where is HMOX1 supposed to be expressed?
T. Anderson
Verified customer
Asked: 2014-05-19
Answer
Based on literature, layer of synovial tissue does express HMOX1. Generally HMOX1 expresses in microsome. Regarding which tissues have HMOX1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 20068231
Foreskin, Pubmed ID: 2911585
Boster Scientific Support
Answered: 2014-05-19
