Click image to see more details
Product Info Summary
| SKU: | PB9278 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Kininogen 1/KNG1 Antibody Picoband®
SKU/Catalog Number
PB9278
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Kininogen 1/KNG1 Antibody Picoband® catalog # PB9278. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Kininogen 1/KNG1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9278)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Kininogen 1 recombinant protein (Position: Q19-N210). Human Kininogen 1 shares 63% and 66% amino acid (aa) sequence identity with mouse and rat Kininogen 1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9278 is reactive to KNG1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
71957 MW
Background of KNG1
Kininogen-1 (KNG1), also known as BDK or bradykinin is a protein that in humans is encoded by the KNG1 gene. It is mapped to 3q27.3. The KNG1 gene uses alternative splicing to generate two different proteins – high – molecular - weight kininogen (HMWK) and low - molecular- weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, KNG1, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. In addition to that, KNG1 is a constituent of the blood coagulation system as well as the kinin-kallikrein system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9278 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human placenta tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Kininogen 1/KNG1 using anti-Kininogen 1/KNG1 antibody (PB9278).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kininogen 1/KNG1 antigen affinity purified polyclonal antibody (Catalog # PB9278) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kininogen 1/KNG1 at approximately 72 kDa. The expected band size for Kininogen 1/KNG1 is at 72 kDa.
Protein Target Info & Infographic
Gene/Protein Information For KNG1 (Source: Uniprot.org, NCBI)
Gene Name
KNG1
Full Name
Kininogen-1
Weight
71957 MW
Alternative Names
Kininogen-1;Alpha-2-thiol proteinase inhibitor;Fitzgerald factor;High molecular weight kininogen;HMWK;Williams-Fitzgerald-Flaujeac factor;Kininogen-1 heavy chain;T-kinin;Ile-Ser-Bradykinin;Bradykinin;Kallidin I;Lysyl-bradykinin;Kallidin II;Kininogen-1 light chain;Low molecular weight growth-promoting factor;KNG1;BDK, KNG; KNG1 BDK, BK, HMWK, KNG kininogen 1 kininogen-1|alpha-2-thiol proteinase inhibitor|bradykinin|fitzgerald factor|high molecular weight kininogen|williams-Fitzgerald-Flaujeac factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KNG1, check out the KNG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KNG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Kininogen 1/KNG1 Antibody Picoband® (PB9278)
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Customer Q&As
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7 Customer Q&As for Anti-Kininogen 1/KNG1 Antibody Picoband®
Question
I have a question about product PB9278, anti-Kininogen 1/KNG1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9278 anti-Kininogen 1/KNG1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-18
Question
Would PB9278 anti-Kininogen 1/KNG1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-07-18
Answer
You can see on the product datasheet, PB9278 anti-Kininogen 1/KNG1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-07-18
Question
I see that the anti-Kininogen 1/KNG1 antibody PB9278 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-05-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-05-03
Question
Is a blocking peptide available for product anti-Kininogen 1/KNG1 antibody (PB9278)?
O. Miller
Verified customer
Asked: 2017-12-11
Answer
We do provide the blocking peptide for product anti-Kininogen 1/KNG1 antibody (PB9278). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-12-11
Question
Is there a BSA free version of anti-Kininogen 1/KNG1 antibody PB9278 available?
Verified Customer
Verified customer
Asked: 2017-10-09
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Kininogen 1/KNG1 antibody PB9278 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-09
Question
Would anti-Kininogen 1/KNG1 antibody PB9278 work on goat WB with cerebrospinal fluid?
Verified Customer
Verified customer
Asked: 2017-09-06
Answer
Our lab technicians have not tested anti-Kininogen 1/KNG1 antibody PB9278 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Kininogen 1/KNG1 antibody PB9278 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat cerebrospinal fluid in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-Kininogen 1/KNG1 antibody PB9278. Let me know if you need anything else.
P. Yang
Verified customer
Asked: 2017-01-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-01-10
