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Product Info Summary
| SKU: | A08428-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-HECTD1 Antibody Picoband®
SKU/Catalog Number
A08428-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HECTD1 Antibody Picoband® catalog # A08428-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-HECTD1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08428-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HECTD1 recombinant protein (Position: D39-S566).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08428-2 is reactive to HECTD1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
290 kDa
Calculated molecular weight
82457 MW
Background of HECTD1
HECTD1 (E3 ubiquitin-protein ligase), which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates,may be required for development of the head mesenchyme and neural tube closure.It is also involved in sporadic Parkinson's disease(PMID:16714300).This protein is specific to HECTD1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08428-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HEK293 whole cell, human Hela whole cell, human HepG2 whole cell
IHC: human hyroid papillary carcinom tissue, human lung cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HECTD1 using anti-HECTD1 antibody (A08428-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HECTD1 antigen affinity purified polyclonal antibody (Catalog # A08428-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HECTD1 at approximately
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Figure 2. IHC analysis of HECTD1 using anti-HECTD1 antibody (A08428-2).
HECTD1 was detected in a paraffin-embedded section of human hyroid papillary carcinom tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HECTD1 Antibody (A08428-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of HECTD1 using anti-HECTD1 antibody (A08428-2).
HECTD1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HECTD1 Antibody (A08428-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For HECTD1 (Source: Uniprot.org, NCBI)
Gene Name
HECTD1
Full Name
E3 ubiquitin-protein ligase HECTD1
Weight
82457 MW
Superfamily
UPL family
Alternative Names
Kallistatin; Kallikrein inhibitor; Peptidase inhibitor 4; PI-4; Serpin A4; SERPINA4; KST; PI4 HECTD1 EULIR HECT domain E3 ubiquitin protein ligase 1 E3 ubiquitin-protein ligase HECTD1|E3 ligase for inhibin receptor|HECT domain containing E3 ubiquitin protein ligase 1|HECT domain-containing protein 1|HECT-type E3 ubiquitin transferase HECTD1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HECTD1, check out the HECTD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HECTD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HECTD1 Antibody Picoband® (A08428-2)
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