Product Info Summary
SKU: | A01843-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-HDAC8 Antibody Picoband™
View all Histone Deacetylase 8/HDAC8 Antibodies
SKU/Catalog Number
A01843-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HDAC8 Antibody Picoband™ catalog # A01843-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-HDAC8 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01843-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HDAC8 recombinant protein (Position: D29-E347).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01843-1 is reactive to HDAC8 in Human, Mouse, Rat
Applications
A01843-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
50 kDa
Calculated molecular weight
Background of Histone Deacetylase 8/HDAC8
HDAC8, Histone deacetylase 8, is an enzyme that in humans is encoded by the HDAC8 gene, and is biologically involved in skull morphogenesis and metabolic control of the ERR-alpha/PGC1-alpha transcriptional complex. Sequence analysis predicted that the 377-amino acid HDAC8 protein contains the 9 conserved HDAC blocks that are presumably important for catalytic function. HDAC8 shares 54% sequence similarity with HDAC1 and HDAC2 and 39% similarity with HDAC3, making it a class I HDAC. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
![a01843 1 hdac8 primary antibodies wb testing 1 a01843 1 hdac8 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a01843-1-hdac8-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of HDAC8 using anti-HDAC8 antibody (A01843-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC8 antigen affinity purified polyclonal antibody (Catalog # A01843-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC8 at approximately 50 kDa. The expected band size for HDAC8 is at 42 kDa.
Protein Target Info & Infographic
Gene/Protein Information For HDAC8 (Source: Uniprot.org, NCBI)
Gene Name
HDAC8
Full Name
Histone deacetylase 8
Weight
Superfamily
histone deacetylase family
Alternative Names
EC 3.5.1.98; HD8; HDAC8; HDACL1; Histone Deacetylase 8; histone deacetylase-like 1; RPD3 HDAC8 CDA07, CDLS5, HD8, HDACL1, KDAC8, MRXS6, RPD3, WTS histone deacetylase 8 histone deacetylase 8|histone deacetylase-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HDAC8, check out the HDAC8 Infographic
![HDAC8 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HDAC8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HDAC8 Antibody Picoband™ (A01843-1)
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