Anti-BMP15 Antibody Picoband™

Product Name

Anti-BMP15 Antibody Picoband™

View all BMP-15/GDF-9B Antibodies

SKU/Catalog Number

PB10048

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BMP15 Antibody Picoband™ catalog # PB10048. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BMP15 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10048)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human BMP15 recombinant protein (Position: Q268-R392). Human BMP15 shares 69.6% amino acid (aa) sequence identity with mouse BMP15.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB10048 is reactive to BMP15 in Human

Applications

PB10048 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

56 kDa

Calculated molecular weight

45055 MW

Background of BMP-15/GDF-9B

Bone morphogenetic protein 15 is a protein that in humans is encoded by the BMP15 gene. It's mainly involved in folliculogenesis. The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The transforming growth factor-beta superfamily includes large families of growth and differentiation factors. It is thought that this protein may be involved in oocyte maturation and follicular development as a homodimer or by forming heterodimers with a related protein, Gdf9. Defects in this gene are the cause of ovarian dysgenesis 2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of BMP15 using anti-BMP15 antibody (PB10048).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMP15 antigen affinity purified polyclonal antibody (Catalog # PB10048) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMP15 at approximately 56 kDa. The expected band size for BMP15 is at 45 kDa.

Gene/Protein Information For BMP15 (Source: Uniprot.org, NCBI)

Alternative Names

BMP15; BMP-15; bone morphogenetic protein 15; GDF-9B; GDF9BODG2; Growth/differentiation factor 9B; POF4 BMP15 GDF9B, ODG2, POF4 bone morphogenetic protein 15 bone morphogenetic protein 15|growth/differentiation factor 9B

For more info on BMP15, check out the BMP15 Infographic

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In this infographic, you will see the following information for BMP15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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